Initial viral load and decay kinetics of SARS-CoV-2 lineage B.1.1.7 in the upper respiratory tract of adults and children

Abstract

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Uncovering strain- and age-dependent innate immune responses to SARS-CoV-2 infection in air-liquid-interface cultured nasal epithelia

“…The elevated viral titers with Alpha-infections compared with WT were expected, due to the increased transmissibility in the variant via enhanced receptor binding affinity due to the N501Y mutation. 7 , 15 Furthermore, viral loads have shown to be higher in Alpha-variant infections than non-VOC strains in other studies, 40 , 41 , 42 , 43 , 44 which appeared to be more evident in adults compared with children, 43 supporting our observations.…”

SARS‐CoV‐2 Delta‐variant breakthrough infections in nursing home residents at midterm after Comirnaty® COVID‐19 vaccination

“…The SARS‐CoV‐2 variant involved in the outbreaks was determined by whole‐genome sequencing at the Foundation for the promotion of Health and Biomedical Research of the Valencian Community (FISABIO), as previously reported. 8 …”

“…SARS‐CoV‐2 viral loads in NP were estimated using the AMPLIRUN® TOTAL SARS‐CoV‐2 RNA Control (Vircell SA), 6 and reported as copies/ml throughout the study. The SARS‐CoV‐2 variant involved in the outbreaks was determined by whole‐genome sequencing at the Foundation for the promotion of Health and Biomedical Research of the Valencian Community (FISABIO), as previously reported 8 …”

SARS-CoV-2 Viral Load Quantification, Clinical Findings and Outcomes in Children Seeking Emergency Department Care: Prospective Cohort Study

“…Although adult data have reported higher viral loads in Alpha VoC samples as measured by Ct and genomic copies, in a retrospective study conducted in Spain, a higher SARS-CoV-2 load was detected in Alpha-infected adults compared to other variants, no such differences were detected in children. 10 …”

“…Although adult data have reported higher viral loads in Alpha VoC samples as measured by Ct and genomic copies, in a retrospective study conducted in Spain, a higher SARS-CoV-2 load was detected in Alpha-infected adults compared to other variants, no such differences were detected in children. 10 Although ddPCR has characteristics that make it analytically superior to RT-PCR, given the capital equipment costs and timeconsuming protocol, we are not proposing that it replace RT-PCR as the standard of care during the COVID-19 pandemic. However, the data we report inform our knowledge about viral loads and RT-PCR test accuracy in our study population.…”