Initial stages of Fagus sylvatica wood colonization by the white-rot basidiomycete Trametes versicolor: Enzymatic characterization

Lekounougou, S.; Mounguengui, Steeve; Dumarçay, Stéphane; Rose, Christophe; Courty, Pierre‐Emmanuel; Garbaye, Jean; Gérardin, Philippe; Jacquot, Jean‐Pierre; Gelhaye, Éric

doi:10.1016/j.ibiod.2007.06.013

Cited by 38 publications

(21 citation statements)

References 36 publications

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“…These observations were completely different from those in Figure 7c (Orange G control) with two peaks at 3.1 min and 3.9 min and absorbance beyond 500 nm. Similar results were presented during a HPLC follow-up of dye biodegradation [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33]. The FTIR spectrum as well as the chromatogram of control (fungi alone) (not presented) were different from those of the degradation products (Figure 6b, 6d, 7b and 7d).…”

Section: Analysis Of Degradation Products By Ftir and Hplcsupporting

confidence: 75%

“…The laccase activity was measured using the method based on the oxidation of ABTS and the measurement of absorbance using a UV-Vis (Varian) Cary Scan Spectrophotometer at 420 nm [19]. Lignin peroxidase (LiP) activity was measured by monitoring the oxidation of veratryl alcohol at 310 nm using the method by Tien and Kirk [20].…”

Section: Ligninolytic Enzymatic Activity On Liquid Mediummentioning

confidence: 99%

“…Lignin peroxidase (LiP) activity was measured by monitoring the oxidation of veratryl alcohol at 310 nm using the method by Tien and Kirk [20]. Manganese peroxidase (MnP) activity was measured according to the method using phenol red at 610 nm in the presence of Mn 2+ [19]. All enzymatic reactions were conducted at 30°C.…”

Section: Ligninolytic Enzymatic Activity On Liquid Mediummentioning

confidence: 99%

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Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Attéké

Mounguengui²,

Jean-Bosco

et al. 2013

J Bioremed Biodeg

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The fungus Pycnoporus sanguineus MUCL 51321 white rot isolated in Gabon showed a high ability to decolorize and degrade two synthetic dyes. On solid and liquid media, the fungus had different enzyme activities (laccase and manganese (Mn) peroxidase) in the presence of different substrates. At concentrations of 0.05 g/L (Orange G) and 0.3 g/L (reactive blue 4), the respective rates of decolorization of 81% and 97% were observed after 15 days of incubation in liquid media. In the same time interval, changes on the spectra (UV-vis and FTIR) and chromatograms (HPLC) showed that these decolorizations were due to the degradation of dyes by fungus with the production of new compounds. The study revealed the possibility of the use of this fungal strain in the microbial degradation process of synthetic dyes. Aldrich (Sigma-Aldrich, St. Louis, MO, USA). All reagents used were of the analytical grade. Journal of Bior emediation & Biodegradation Culture ConditionsThe composition of the solid growth medium used was as follows: 0.2% glucose, 0.2% malt extract, 0.01% MgSO 4 , 0.01% MnCl 2 , 0.03% NH 4 NO 3 , 0.026% KH 2 PO 4 , 0.026% Na 2 HPO 4 , 0.01% CaCl 2 .2H 2 O, 0.0001% FeC1 3 .6H 2 O, 0.0001% ZnCl 2 , 1.6% and 1.6% agar in 1 L of distilled water. The pH was adjusted to 5.5 with hydrochloric acid (0. 5 N) before autoclaving (at 121°C for 15 min). The culture medium was transferred into 4 Erlenmeyer flasks (200 mL each) and each reagent (enzymatic revelation) or dye, depending on its final concentration was added. The mixture was shaken, then about 15 mL were distributed in a Petri dish (diameter 8.5 mm); each test was replicated three times. 50 mL of liquid culture media in flasks of 100 mL were constituted in almost the same way as the solid culture media described above, except for the absence of agar. Each test was conducted three times. Decolorization experimentsDyes decolorization on solid medium: Solid culture media contained different concentrations of each dye (0.05 g/L to 1.5 g/L). Various controls were also prepared: some Petri dishes with fungus alone and others with dye alone to assess possible abiotic decolorization. One square portion of agar (1 cm of diameter) containing the fungus was placed at the centre of each Petri dish. The fungal growth diameters and the halos of decolorization were measured every day during one week at constants temperature (30°C) and humidity (75%). After these measures, different ratios and rates (decolorization or inhibition) were determined as described by . The optimum temperature of decolorization was evaluated using different growth temperatures (15, 22, 30, 35, 40 and 45°C). Moreover, the optimal concentrations for decolorization (0.05 g/L for the Orange G and 0.3 g/L for the RB4) were obtained after one week. All tests were conducted three times (averages are presented with the corresponding standard deviations).Dyes decolorization on liquid culture medium: For each Erlenmeyer flask (100 mL) containing liquid culture medium (50 mL) and dye (0.05 g/L Orange G or 0.3...

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Section: Analysis Of Degradation Products By Ftir and Hplcsupporting

confidence: 75%

Section: Ligninolytic Enzymatic Activity On Liquid Mediummentioning

confidence: 99%

Section: Ligninolytic Enzymatic Activity On Liquid Mediummentioning

confidence: 99%

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Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Attéké

Mounguengui²,

Jean-Bosco

et al. 2013

J Bioremed Biodeg

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“…This is striking since both the understanding of the decay process in fungus-colonised wood and the factors affecting fruitbody formation are essential for the understanding of the physiology of fungi causing biodeterioration of wood and may help both to develop preventive measures to reduce fungus-induced wood losses and to undertake measures for the conservation of endangered woodassociated fungi (Dahlberg et al 2010). In previous papers, enzyme activity in wood was mostly studied under artificial laboratory conditions (e.g., Ruel et al 1994;Lekounougou et al 2008). Only recently, papers on enzyme activities in the environmental samples of wood colonised by brown-rot and whiterot fungi appeared, demonstrating high activities of several hydrolytic and oxidative enzymes in this environment (Valášková and Baldrian 2006;Valáš-ková et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Ecology of coarse wood decomposition by the saprotrophic fungus Fomes fomentarius

et al. 2010

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Saprotrophic wood-inhabiting basidiomycetes are the most important decomposers of lignin and cellulose in dead wood and as such they attracted considerable attention. The aims of this work were to quantify the activity and spatial distribution of extracellular enzymes in coarse wood colonised by the white-rot basidiomycete Fomes fomentarius and in adjacent fruitbodies of the fungus and to analyse the diversity of the fungal and bacterial community in a fungus-colonised wood and its potential effect on enzyme production by F. fomentarius. Fungus-colonised wood and fruitbodies were collected in low management intensity forests in the Czech Republic. There were significant differences in enzyme production by F. fomentarius between Betula pendula and Fagus sylvatica wood, the activity of cellulose and xylan-degrading enzymes was significantly higher in beech wood than in birch wood. Spatial analysis of a sample B. pendula log segment proved that F. fomentarius was the single fungal representative found in the log. There was a high level of spatial variability in the amount of fungal biomass detected, but no effects on enzyme activities were observed. Samples from the fruiting body showed high β-glucosidase and chitinase activities compared to wood samples. Significantly higher levels of xylanase and cellobiohydrolase were found in samples located near the fruitbody (proximal), and higher laccase and Mn-peroxidase activities were found in the distal ones. The microbial community in wood was dominated by the fungus (fungal to bacterial DNA ratio of 62-111). Bacterial abundance composition was lower in proximal than distal parts of wood by a factor of 24. These results show a significant level of spatial heterogeneity in coarse wood. One of the explanations may be the successive colonization of wood by the fungus: due to differential enzyme production, the rates of biodegradation of coarse wood are also spatially inhomogeneous.

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“…Catechin is the most frequently reported fl avonoid that can be extracted from the beech wood ( Figure 7). A few research groups reported on the presence of catechin in the sapwood and discoloured wood (Kubel and Weissmann, 1988 Lekounougou et al, 2008). It was reported that the amount of catechin in discoloured tissues decreases due to its participation in the process of formation of colour chromophores (Hofmann et al, 2004).…”

Section: Hydrophilic Extractivesmentioning

confidence: 99%

Review on Lipophilic and Hydrophilic Extractives in Tissues of Common Beech

2016

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Initial stages of Fagus sylvatica wood colonization by the white-rot basidiomycete Trametes versicolor: Enzymatic characterization

Cited by 38 publications

References 36 publications

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Ecology of coarse wood decomposition by the saprotrophic fungus Fomes fomentarius

Review on Lipophilic and Hydrophilic Extractives in Tissues of Common Beech

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