Initial research on the relationship between let-7 family members in the serum and massive cerebral infarction

Gong, Zhe; Zhao, Shaoyun; Zhang, Jing; Xu, Xiaoge; Guan, Wenjuan; Jing, Lijun; Liu, Peidong; Lu, Jingjing; Teng, Jie; Peng, Tao; Jia, Yanjie

doi:10.1016/j.jns.2015.12.047

Cited by 21 publications

(12 citation statements)

References 58 publications

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“…The endpoint of qRT-PCR data is the comparative cycle threshold method (Ct). The relative changes in gene expression were quantified using the Livak method (also known as 2 −ΔΔCt method) after determining the Ct values for the reference and target genes in each sample set [ 18 ]. All reactions were repeated for three times.…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial dysfunction induces NLRP3 inflammasome activation during cerebral ischemia/reperfusion injury

Gong

Pan

Shen

et al. 2018

J Neuroinflammation

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BackgroundNod-like receptor protein 3 (NLRP3) inflammasome is a crucial factor in mediating inflammatory responses after cerebral ischemia/reperfusion (I/R), but the cellular location of NLRP3 inflammasome in cerebral I/R has yet come to a conclusion, and there is still no specific evidence to state the relationship between mitochondria and the NLRP3 inflammasome in cerebral I/R.MethodsIn the present study, we detected the cellular localization of NLRP3 inflammasomes in a transient middle cerebral artery occlusion (tMCAO) rat model and a transwell co-culture cell system under oxygen-glucose deprivation/reoxygenation (OGD/R) conditions. Then, we investigated the relationship between mitochondrial dysfunction and the activation of NLRP3 inflammasomes in different cell types after OGD/R and cerebral I/R injury.ResultsOur results showed that NLRP3 inflammasomes were first activated in microglia soon after cerebral I/R injury onset and then were expressed in neurons and microvascular endothelial cells later, but they were mainly in neurons. Furthermore, mitochondrial dysfunction played an important role in activating NLRP3 inflammasomes in microglia after OGD/R, and mitochondrial protector could inhibit the activation of NLRP3 inflammasomes in cerebral I/R rats.ConclusionOur findings may provide novel insights into the cell type-dependent activation of NLRP3 inflammasomes at different stages of cerebral I/R injury and the role of mitochondrial dysfunction in activating the NLRP3 inflammasome pathway.Electronic supplementary materialThe online version of this article (10.1186/s12974-018-1282-6) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Mitochondrial dysfunction induces NLRP3 inflammasome activation during cerebral ischemia/reperfusion injury

Gong

Pan

Shen

et al. 2018

J Neuroinflammation

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224

124

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“…Another important finding of the study showed that the expression level of let-7f in the MCI without HT is positively correlated with patients status based on Glasgow Coma Scale score and negatively with hs-CRP concentration (r = −0.88, p < 0.0001). Also in this study target gene expression analysis was performed and the relative expression of let-7f was negatively correlated with interleukin-6 (IL-6) expression in the MCI without HT (48 h and 2 weeks) (r = −0.40, p < 0.001), but not in the MCI with HT group, which may suggest that the downregulation of let-7f expression in patients with MCI without HT may induce inflammation [45]. The large involvement of let-7 with cerebrovascular disease, as proven by multiple evidences from independent studies is particular interesting.…”

Section: Circulating Mirnas and Strokementioning

confidence: 99%

“…Abbreviation: miRNA, microRNA; CRP, C-reactive protein; HDL, high density lipoprotein; NIHSS, National Institutes of Health Stroke Scale; IL-6, interleukin 6; TOAST, Trial of Org 10,172 in Acute Stroke Treatment; OCSP, Oxfordshire Community Stroke Project; HACI, hyperacute cerebral infarction; mRS, Modified Rankin Scale; BI, Barthel index. Refs: [41,43,44,45,47,48,53,55,57,58,61,62,67,68,72,73,74,75,76,78].…”

Section: Figurementioning

confidence: 99%

MicroRNAs as Diagnostic and Prognostic Biomarkers in Ischemic Stroke—A Comprehensive Review and Bioinformatic Analysis

Eyileten

Wicik

Rosa

et al. 2018

Cells

126

104

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Stroke is the second-most common cause of death worldwide. The pathophysiology of ischemic stroke (IS) is related to inflammation, atherosclerosis, blood coagulation, and platelet activation. MicroRNAs (miRNAs) play important roles in physiological and pathological processes of neurodegenerative diseases and progression of certain neurological diseases, such as IS. Several different miRNAs, and their target genes, are recognized to be involved in the pathophysiology of IS. The capacity of miRNAs to simultaneously regulate several target genes underlies their unique value as diagnostic and prognostic markers in IS. In this review, we focus on the role of miRNAs as diagnostic and prognostic biomarkers in IS. We discuss the most common and reliable detection methods available and promising tests currently under development. We also present original results from bioinformatic analyses of published results, identifying the ten most significant genes (HMGB1, YWHAZ, PIK3R1, STAT3, MAPK1, CBX5, CAPZB, THBS1, TNFRSF10B, RCOR1) associated with inflammation, blood coagulation, and platelet activation and targeted by miRNAs in IS. Additionally, we created miRNA-gene target interaction networks based on Gene Ontology (GO) information derived from publicly available databases. Among our most interesting findings, miR-19a-3p is the most widely modulated miRNA across all selected ontologies and might be proposed as novel biomarker in IS to be tested in future studies.

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“…In contrast, the content of let-7c-5p is significantly reduced in the plasma of IS patients and the ipsilateral cortex and striatum of MCAO mice at 24 hours reperfusion, which concurs with increased activation of microglia [127]. While another member of let-7 family, let-7f, is reported to be significantly down-regulated in sera of massive cerebral infarction without hemorrhagic transformation (HT) patients within 48 hours of a stroke compared with controls, leading to an increased serum IL-6 level [128]. Additionally, let-7i is reported to be decreased in circulating leukocytes of patients with acute IS within 72 hours and to be associated with increased expression of its mRNA targets including CD86, CXCL8, HMGB1, indicating its involvement in leukocyte activation, recruitment and proliferation [129].…”

Section: Candidate Mirna Markersmentioning

confidence: 69%

Monogenic, Polygenic, and MicroRNA Markers for Ischemic Stroke

Chen

Sinha

et al. 2018

Mol Neurobiol

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Ischemic stroke (IS) is a leading disease with high mortality and disability, as well as with limited therapeutic window. Biomarkers for earlier diagnosis of IS have long been pursued. Family and twin studies confirm that genetic variations play an important role in IS pathogenesis. Besides DNA mutations found previously by genetic linkage analysis for monogenic IS (Mendelian inheritance), recent studies using genome-wide associated study (GWAS) and microRNA expression profiling have resulted in a large number of DNA and microRNA biomarkers in polygenic IS (sporadic IS), especially in different IS subtypes and imaging phenotypes. The present review summarizes genetic markers discovered by clinical studies and discusses their pathogenic molecular mechanisms involved in developmental or regenerative anomalies of blood vessel walls, neuronal apoptosis, excitotoxic death, inflammation, neurogenesis, and angiogenesis. The possible impact of environment on genetics is addressed as well. We also include a perspective on further studies and clinical application of these IS biomarkers.

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Initial research on the relationship between let-7 family members in the serum and massive cerebral infarction

Cited by 21 publications

References 58 publications

Mitochondrial dysfunction induces NLRP3 inflammasome activation during cerebral ischemia/reperfusion injury

Mitochondrial dysfunction induces NLRP3 inflammasome activation during cerebral ischemia/reperfusion injury

MicroRNAs as Diagnostic and Prognostic Biomarkers in Ischemic Stroke—A Comprehensive Review and Bioinformatic Analysis

Monogenic, Polygenic, and MicroRNA Markers for Ischemic Stroke

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