Initial Phases of DNA Rehydration by NMR and Sorption Isotherm

Harańczyk, H.; Czak, J.; Nowak, Paweł; Nizioł, Jacek

doi:10.12693/aphyspola.117.397

Cited by 19 publications

(10 citation statements)

References 28 publications

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“…Relaxation time for the solid component,

≈21 μs, is close to the value obtained for other lichens, and different solid biological systems as lyophilized photosynthetic membranes [1] , [2] , lyophilized model membranes from DGDG [23] , or lyophilized DNA [41] , which suggests similar distribution of local magnetic fields for limited number of chemical groups forming the biological system at a submolecular scale [42] .…”

Section: Resultssupporting

confidence: 80%

A method of water-soluble solid fraction saturation concentration evaluation in dry thalli of Antarctic lichenized fungi, in vivo

Harańczyk

Nowak

Lisowska

et al. 2016

Biochemistry and Biophysics Reports

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BackgroundAt initial steps of rehydration from cryptobiosis of anhydrobiotic organisms or at rehydration of dry tissues the liquid 1H NMR signal increased anomaly. The surplus in liquid signal may appear if some solid constituents dissolved, or if they were decomposed by enzymatic action.MethodsHydration kinetics, sorption isotherm, 1H NMR spectra and high power relaxometry were applied to monitor gaseous phase rehydration of Antarctic lichen Cetraria aculeata. Tightly and loosely bound water signal were distinguished, and the upper hydration limit for dissolution of water soluble solid fraction was not observed. A simple theoretical model was proposed.ResultsThe hydration courses showed a very tightly bound water fraction, a tightly bound water, and a loosely bound water fraction. Sigmoidal in form sorption isotherm was fitted well by multilayer sorption model. 1H NMR showed one Gaussian signal component from solid matrix of thallus and one or two Lorentzian line components from tightly bound, and from loosely bound water. The hydration dependency of liquid signal was fitted by rational function.ConclusionsAlthough in dehydrated C.aculeata the level of carbohydrates and polyols was low, the lichenase action during rehydration process increased it; the averaged saturation concentration cs=(57.3±12.0)%, which resembled that for sucrose.General significanceThe proposed method of water soluble solid fraction saturation concentration, cs, calculation from 1H NMR data may be applied for other organisms experiencing extreme dehydration or for dry tissues. We recalculated the published elsewhere data for horse chestnut (Aesculus hippocastanum) bast [water-soluble solid fraction recognized as sucrose, cs=(74.5±5.1)%]; and for Usnea antarctica, where cs=0.81±0.04.

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“…Relaxation time for the solid component,

Section: Resultssupporting

confidence: 80%

A method of water-soluble solid fraction saturation concentration evaluation in dry thalli of Antarctic lichenized fungi, in vivo

Harańczyk

Nowak

Lisowska

et al. 2016

Biochemistry and Biophysics Reports

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“…The L 1 signal component with the value T * 2L 1 ≈ 100 µs is characteristic of tightly bound water fraction either lichen thalli or for many other biological systems [19,[22][23][24][25][26][27][28][29][30]. The L 2 signal with T * 2L 2 ≈ 330 µs, shortened by B 0 inhomogeneities [31], comes from water loosely bound on thallus surfaces and/or from free water fraction (Fig.…”

Section: Resultsmentioning

confidence: 99%

Initial Phases of Antarctic Ramalina terebrata Hook f. & Taylor Thalli Rehydration Observed by Proton Relaxometry

et al. 2012

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Hydration kinetics, sorption isotherm, and proton free induction decays are measured for Ramalina terebrata thalli rehydrated from gaseous phase. Very tightly, tightly, and loosely bound water fractions are distinguished. Sorption isotherm is sigmoidal in form with the mass of water saturating primary water binding sites equal to ∆m/m 0 = 0.046. Proton free induction decays show the presence of immobilized water fraction (T * 2L 1 ≈ 100 µs) and mobile water pool (T * 2L 2 ≈ 330 µs). Sorption isotherm fitted to the NMR data shows the absence of water fraction "sealed" in pores of dry thallus.

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“…The amplitude of the swelling was much smaller than for salmon sperm DNA and started after significantly shorter delay [8]. This may suggest differences in outer surface of the DNA-CTMA lyophilizate grains (Fig.…”

Section: Swellingmentioning

confidence: 89%

“…The constant A h 2 increases gradually with the relative humidity of the atmosphere. The third exponential in hydration kinetics curve was not observed, as it was in salmon sperm DNA not treated by the use of surfactant [8]. …”

Section: Hydration Kineticsmentioning

confidence: 91%