Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue‐engineered vascular grafts
            <i>in vivo</i>

Nelson, Gregory N.; Roh, Jason D.; Mirensky, Tamar L.; Wang, Y.; Yi, Tai; Tellides, George; Pober, Jordan S.; Shkarin, Pavel; Shapiro, Erik M.; Saltzman, W. Mark; Papademetris, Xenios; Fahmy, Tarek M.; Breuer, Christopher K.

doi:10.1096/fj.08-107367

Cited by 39 publications

(27 citation statements)

References 29 publications

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“…MRI was utilized to evaluate structure and function of the human TEVGs in addition to investigating the fate of the cells in vivo over a 3 week time course (89). Especially important is gathering information on the fate of tissue-engineered endothelium in a vascular graft, as this cell layer provides an anticoagulant and antithrombotic barrier, modulates vascular tone, growth, inflammation and haemostasis by secreting biologically active mediators.…”

Section: Nanoparticles As Diagnostic Probesmentioning

confidence: 99%

“…The nanoparticle labeling allowed an accurate delineation of the mesh upon implantation (arrow). D) Non-invasive MR monitoring of a tissue-engineered vascular graft (TEVGs) in vivo, colonized with iron oxide-labeled smooth muscle cells (from (89) with permission). The labeled cells facilitated the evaluation of graft localization (arrowheads), structure and function over a 3 week time course.…”

Section: Essentialsmentioning

confidence: 99%

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Nanoparticles for Imaging: Top or Flop?

Kießling¹,

Mertens²,

Grimm³

et al. 2014

Radiology

191

146

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Nanoparticles are frequently suggested as diagnostic agents. However, except for iron oxide nanoparticles, diagnostic nanoparticles have been barely incorporated into clinical use so far. This is predominantly due to difficulties in achieving acceptable pharmacokinetic properties and reproducible particle uniformity as well as to concerns about toxicity, biodegradation, and elimination. Reasonable indications for the clinical utilization of nanoparticles should consider their biologic behavior. For example, many nanoparticles are taken up by macrophages and accumulate in macrophage-rich tissues. Thus, they can be used to provide contrast in liver, spleen, lymph nodes, and inflammatory lesions (eg, atherosclerotic plaques). Furthermore, cells can be efficiently labeled with nanoparticles, enabling the localization of implanted (stem) cells and tissue-engineered grafts as well as in vivo migration studies of cells. The potential of using nanoparticles for molecular imaging is compromised because their pharmacokinetic properties are difficult to control. Ideal targets for nanoparticles are localized on the endothelial luminal surface, whereas targeted nanoparticle delivery to extravascular structures is often limited and difficult to separate from an underlying enhanced permeability and retention (EPR) effect. The majority of clinically used nanoparticle-based drug delivery systems are based on the EPR effect, and, for their more personalized use, imaging markers can be incorporated to monitor biodistribution, target site accumulation, drug release, and treatment efficacy. In conclusion, although nanoparticles are not always the right choice for molecular imaging (because smaller or larger molecules might provide more specific information), there are other diagnostic and theranostic applications for which nanoparticles hold substantial clinical potential.

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Section: Nanoparticles As Diagnostic Probesmentioning

confidence: 99%

Section: Essentialsmentioning

confidence: 99%

Nanoparticles for Imaging: Top or Flop?

Kießling¹,

Mertens²,

Grimm³

et al. 2014

Radiology

191

146

View full text Add to dashboard Cite

show abstract

“…For fluorescence microscopic analyses, ECs and PCs were labeled with PKH67 green florescence lipophilic membrane dye and PKH26 red florescence lipophilic membrane dye (both from Sigma-Aldrich, Saint Louis, MO, USA), respectively, as per manufacturer’s instructions. To label PCs for identification by transmission electron microscopy we utilized Molday ION Rhodamine B (BioPAL, Worcester, MA, USA) an ultrasmall paramagnetic iron oxide nanoparticle (USPIO) of 35 nm diameter that is positively charged for enhanced cellular uptake, as described by others[12,13]. In brief, 100,000 PCs were plated in the well of a 24 well tissue culture plate in 0.5ml of M199 containing 20%FBS, cultured overnight and then incubated for an additional 16 hours under standard growth conditions (5% CO 2 , at 37° C) with 200ug/ml of Molday ION Rhodamine B USPIOs, a concentration permitting optimal uptake by PCs as judged by Rhodamine B fluorescence.…”

Section: Methodsmentioning

confidence: 99%

In vitro Self-Assembly of Human Pericyte-Supported Endothelial Microvessels in Three-Dimensional Coculture: A Simple Model for Interrogating Endothelial-Pericyte Interactions

Waters

Kluger²,

Graham

et al. 2013

J Vasc Res

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We describe a method for coculture of macro- or microvascular human endothelial cells (ECs) and pericytes (PCs) within a 3-dimensional (3-D) protein matrix resulting in lumenized EC cords invested by PCs. To prevent apoptotic cell death of ECs in 3-D culture, human umbilical vein or dermal microvascular ECs were transduced to express the antiapoptotic protein Bcl-2. To prevent PC-mediated gel contraction, the collagen-fibronectin gel was polymerized within a polyglycolic acid nonwoven matrix. Over the first 24-48 h, EC-only gels spontaneously formed cords that developed lumens via vacuolization; such vascular networks were maintained for up to 7 days. In EC-PC cocultures, PCs were recruited to the EC networks. PC investment of EC cords both limited the lumen diameter and increased the degree of vascular network arborization. Peg and socket junctions formed between ECs and PCs in this system, but dye transfer, indicative of gap junction formation, was not observed. This simple system can be used to analyze bidirectional signals between ECs and PCs in a 3-D geometry.

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“…Studies demonstrated the potential of MRI for monitoring tissue-engineered vascular grafts in vivo using human aortic smooth muscle cells labeled with ultrasmall iron oxide nanoparticles (USPIO). MRI here was used to longitudinally evaluate the structure and function of the human tissue-engineered grafts in addition to investigating the fate of the cells upon implantation [46]. Especially important is also the fate of tissueengineered endothelium in a vascular graft, as it provides an anticoagulant and antithrombotic barrier, modulating vascular tone, growth, inflammation, and hemostasis by secreting biologically active mediators.…”

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

Nondestructive monitoring of tissue-engineered constructs

Frese

Morgenroth

Mertens

et al. 2014

Biomedical Engineering / Biomedizinische Technik

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Tissue engineering as a multidisciplinary field enables the development of living substitutes to replace, maintain, or restore diseased tissue and organs. Since the term was introduced in medicine in 1987, tissue engineering strategies have experienced significant progress. However, up to now, only a few substitutes were able to overcome the gap from bench to bedside and have been successfully approved for clinical use. Substantial donor variability makes it difficult to predict the quality of tissue-engineered constructs. It is essential to collect sufficient data to ensure that poor or immature constructs are not implanted into patients. The fulfillment of certain quality requirements, such as mechanical and structural properties, is crucial for a successful implantation. There is a clear need for new nondestructive and real-time online monitoring and evaluation methods for tissue-engineered constructs, which are applicable on the biomaterial, tissue, cellular, and subcellular levels. This paper reviews current established nondestructive techniques for implant monitoring including biochemical methods and noninvasive imaging.

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Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue‐engineered vascular grafts in vivo

Cited by 39 publications

References 29 publications

Nanoparticles for Imaging: Top or Flop?

Nanoparticles for Imaging: Top or Flop?

In vitro Self-Assembly of Human Pericyte-Supported Endothelial Microvessels in Three-Dimensional Coculture: A Simple Model for Interrogating Endothelial-Pericyte Interactions

Nondestructive monitoring of tissue-engineered constructs

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