Initial enamel crystals are not spatially associated with mineralized dentine

Diekwisch, Thomas G. H.; Berman, Brett J.; Gentner, Steven; Slavkin, Harold C.

doi:10.1007/s004410050271

Cited by 26 publications

(30 citation statements)

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“…Some authors claim that the enamel crystals grow epitaxially on the preexisting dentin crystals because of a high continuity between enamel and dentin crystals (Arsenault and Robinson 1989;Hayashi 1993). Others have shown that enamel crystals are formed at a given distance from the dentin surface (Diekwisch et al 1998) and could either reach dentin crystals by a subsequent growth (Takano et al 1996) or remain distant (Dong and Warshawsky 1996). In the present study, we found only two types of crystallographic orientations between enamel and dentin crystals, and in both cases no direct contact between particle types was found (Figs.…”

Section: Discussioncontrasting

confidence: 33%

“…Enamel crystals were described as growing either epitaxially on the preexisting dentin crystals because of a high continuity between enamel and dentin crystals (Arsenault and Robinson 1989;Hayashi 1993) or at a distance from the dentin surface (Diekwisch et al 1998) followed by subsequent growth to bring them into contact with dentin crystals (Takano et al 1996). The relationship between enamel and dentin should be thus investigated to highlight, during enamel formation, the guiding or modulating role of the previously mineralized dentin layer.…”

Section: Introductionmentioning

confidence: 99%

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High-resolution electron-microscopic study of the relationship between human enamel and dentin crystals at the dentinoenamel junction

Bodier-Houllé

Steuer

Meyer

et al. 2000

Cell and Tissue Research

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The development of dentin and of enamel share a common starting locus: the dentinoenamel junction (DEJ). In this study the relationship between enamel and dentin crystals has been investigated in order to highlight the guiding or modulating role of the previously mineralized dentin layer during enamel formation. Observations were made with a high-resolution electron microscope and, after digitalization, image-analysis software was used to obtain digital diffractograms of individual crystals. In general no direct epitaxial growth of enamel crystals onto dentin crystals could be demonstrated. The absence of direct contact between the two kinds of crystals and the presence of amorphous areas within enamel particles at the junction with dentin crystals were always noted. Only in a few cases was the relationship between enamel and dentin crystals observed, which suggested a preorganization of the enamel matrix influenced by the dentin surface structure. This could be explained either by the existence of a proteinaceous continuum between enamel and dentin or by the orientation of enamel proteins by dentin crystals.

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Section: Discussioncontrasting

confidence: 33%

Section: Introductionmentioning

confidence: 99%

High-resolution electron-microscopic study of the relationship between human enamel and dentin crystals at the dentinoenamel junction

Bodier-Houllé

Steuer

Meyer

et al. 2000

Cell and Tissue Research

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“…7(D)] closely re- sembled the apatite crystals observed in the early stage of enamel biomineralization. 31,32 The modulation of rP172 on apatite crystals grown on Bioglass confirmed our recent observation of the elongating effect of both recombinant and native porcine amelogenins on the calcium phosphate crystals formed in solution and gel. 24,25 These findings verified to a large extent the hypothesis on the function of amelogenin assemblies in preventing premature lateral crystalcrystal fusions while promoting the c-axial crystal growth of the crystals throughout the full thickness of the secretory matrix.…”

Section: Surface Transformation In Scs Rp172supporting

confidence: 69%

“…The long and thin crystals shown in Figure 7(B,D) and in particular those in Figure 7(D) with a highly ordered crystal arrangement are similar to the apatite crystals formed in the early stage of enamel biomineralization. 31,32 …”

Section: Temmentioning

confidence: 99%

Effects of amelogenin on the transforming surface microstructures of Bioglass� in a calcifying solution

Wen

Moradian‐Oldak

Zhong³

et al. 2000

J. Biomed. Mater. Res.

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Topographies of a bioactive glass (45S5 type Bioglass(R)) during 0-4 h of immersion in a supersaturated calcifying solution (SCS) and the SCS containing recombinant porcine amelogenin rP172 (SCS(rP172)) were observed by atomic force microscopy. Other techniques including X-ray diffraction, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy, and transmission electron microscopy were used for some complementary microstructural investigations. The smooth Bioglass surface changed to be very rough after 0.5 h of SCS immersion because of glass network dissolution. Spherical silica-gel particles with diameters of 150-300 nm consisting of substructures of 20-60 nm across had formed on the sample surfaces after 1 h of SCS immersion. The chemisorption of amorphous calcium phosphate and crystallization of nanophase apatite were seen to occur epitaxially on the silica-gel structures during 1-4 h of SCS immersion. During the first 0.5 h of SCS(rP172) immersion, more than 95% of rP172 protein in solution was adsorbed onto the sample surfaces and generated spherical assemblies of 10-60 nm diameters. During 0.5-4 h of SCS(rP172) immersion, the protein assemblies of rP172 remarkably induced the formation of orientated silica-gel plates (approximately 100-nm wide and 50-nm thick) and subsequently of long and thin apatite needle crystals. The recombinant amelogenin rP172-modulated apatite crystals resembled those formed in the early stage of tooth enamel biomineralization, suggesting the functional roles of amelogenins during the oriented growth of enamel crystallites and a great potential for amelogenins in applications designed to fabricate enamel-like calcium phosphate biomaterials.

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“…whereas others described enamel crystal growth at a certain distance from dentine (Diekwiseh et al, 1995). These differences might reside in the topology of DEJ which, in ultrathin sections, could sbow dentine crystals inside enamel territory and in the low crystallinity of young developing crystals which could be obscured by thicker ones.…”

mentioning

confidence: 90%

Initial aspects of mineralization at the dentino-enamel junction in embryonic mouse incisor in vivo and in vitro: A tem comparative study

Meyer

Bodier-Houllé

Cuisinier

et al. 1999

In Vitro Cell.Dev.Biol.-Animal

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The frontier between the enamel organ and the dental papilla, the future dentino-enamel junction, undergoes coordinated modifications. The mineralization of the extracellular matrix starts within the predentine, which is a prerequisite for the formation of the first enamel crystallites in vivo. We investigated the dentino-enamel junction using the embryonic mouse incisor as a model. Our data showed that the notion of the dentino-enamel junction should not be restricted to the thin interface classically described. A temporo-spatial survey from the epithelio-mesenchymal junction to the dentino-enamel junction delineated a clear sequence of events characterized by the early deposition of electron-dense granules, followed by the appearance of patches of stippled material at the dentino-enamel junction. The first tiny enamel crystallites appeared in the vicinity of this material which presented a well-ordered alignment. The comparison of data obtained in vivo on 17-, 18-, 19-d-old embryonic incisors with those obtained in vitro using 15-d-old embryonic incisors cultured for 7 d emphasizes the relevance of this sequence. Helicoidal growing crystals were observed in cultured tooth germs but never in vivo.

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Initial enamel crystals are not spatially associated with mineralized dentine

Cited by 26 publications

References 0 publications

High-resolution electron-microscopic study of the relationship between human enamel and dentin crystals at the dentinoenamel junction

High-resolution electron-microscopic study of the relationship between human enamel and dentin crystals at the dentinoenamel junction

Effects of amelogenin on the transforming surface microstructures of Bioglass� in a calcifying solution

Initial aspects of mineralization at the dentino-enamel junction in embryonic mouse incisor in vivo and in vitro: A tem comparative study

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