Initial determination of DNA polymorphism of some Primula veris L. populations from Kosovo and Austria

Abstract

Primula veris L. (Primulaceae) is a long lived perennial and well known pharmaceutical plant, widely collected for these reasons in almost all SE Europe and particularly in Kosovo. The aim of the study is to determine molecular polymorphism of cowslip (P. veris L.) populations from Kosovo. DNA extracted from leaves were investigated in details for presence of polymorphism. RAPD analyses were conducted using 20 different short primers. Genomic DNA amplification profiles were analyzed and processed using data labelling. Comparison between cowslip populations in genetic composition revealed that samples from Bogaj were too distinct on their own. Molecular variation was observed to be more within populations (73 %) as compared to among populations (27 %). On the other hand, genetic distance of populations revealed that the highest genetic distance is between Leqinat and Maja e Madhe. Mean values of expected heterozygosity were highest in Bogaj population, while lowest in Maja e Madhe population. The obtained results indicated that Bogaj population are more polymorphic. From the obtained data it can be concluded that RAPD markers provided a useful technique to study genetic diversity in P. veris L. populations. This technology allows identification and assessment of the genetic similarities and differences among plant populations.

Conservation of Primula farinosa in Poland with respect to the genetic structure of populations

“…Our between-population and within-population variation proportions, 18.53% and 81.47%, respectively, very closely matched the results of Reisch et al [34] (20.59% and 79.41%, respectively) for populations located near Obersdorf (northern calcareous Alps, Germany) and were only slightly different from those of Sørensen et al [35] (33% and 67%, respectively) obtained for populations collected throughout Denmark. Similar results were obtained for other Primula species: P. apennina [37] and P. veris [38]. Both basic genetic indexes used in our study show that the Polish population PL is the most variable of the four studied.…”

Primula veris plants derived from in vitro cultures and from seeds: genetic stability, morphology, and seed characteristics

“…Primula veris L. (Primulaceae, also called cowslip) is a distylous outcrossing grassland herb characterized by declining number and size of populations in recent years. This has caused an increased interest in the species, with the goal of saving its contribution to biological diversity (Kéry et al, 2000;Brys and Jacquemyn, 2009;Berisha et al, 2015). One of the reasons causing the reduced number of cowslip populations may be very low level of genetic variation found in natural populations (Morozowska and Krzakowa, 2003).…”