Initial characteristics of RbcX proteins from Arabidopsis thaliana

Kolesinski, Piotr; Piechota, Janusz; Szczepaniak, Andrzej

doi:10.1007/s11103-011-9823-8

Cited by 29 publications

(28 citation statements)

References 35 publications

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“…7B). OsAP37-activated genes such as ICL, RLD, E3UL, RcbX, and Dfu584 are known to play a crucial role in carbon metabolism and photosynthesis under drought stress (Kwak et al, 2007;Lee et al, 2009;Kolesi nski et al, 2011;Luhua et al, 2013;Wang et al, 2014). The high induction under drought of GUDK in the flag leaf and reduced photosynthesis in the mutant lines suggest that GUDK regulates photosynthesis and carbon metabolism genes by phosphorylating OsAP37, Table I.…”

Section: Gudk Regulates Rice Yield By Potentially Transphosphorylatinmentioning

confidence: 99%

Rice GROWTH UNDER DROUGHT KINASE Is Required for Drought Tolerance and Grain Yield under Normal and Drought Stress Conditions

et al. 2014

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Rice (Oryza sativa) is the primary food source for more than one-half of the world's population. Because rice cultivation is dependent on water availability, drought during flowering severely affects grain yield. Here, we show that the function of a drought-inducible receptor-like cytoplasmic kinase, named GROWTH UNDER DROUGHT KINASE (GUDK), is required for grain yield under drought and well-watered conditions. Loss-of-function gudk mutant lines exhibit sensitivity to salinity, osmotic stress, and abscisic acid treatment at the seedling stage, and a reduction in photosynthesis and plant biomass under controlled drought stress at the vegetative stage. The gudk mutants interestingly showed a significant reduction in grain yield, both under normal well-watered conditions and under drought stress at the reproductive stage. Phosphoproteome profiling of the mutant followed by in vitro assays identified the transcription factor APETALA2/ETHYLENE RESPONSE FACTOR OsAP37 as a phosphorylation target of GUDK. The involvement of OsAP37 in regulating grain yield under drought through activation of several stress genes was previously shown. Our transactivation assays confirmed that GUDK is required for activation of stress genes by OsAP37. We propose that GUDK mediates drought stress signaling through phosphorylation and activation of OsAP37, resulting in transcriptional activation of stress-regulated genes, which impart tolerance and improve yield under drought. Our study reveals insights around drought stress signaling mediated by receptorlike cytoplasmic kinases, and also identifies a primary regulator of grain yield in rice that offers the opportunity to improve and stabilize rice grain yield under normal and drought stress conditions.

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Section: Gudk Regulates Rice Yield By Potentially Transphosphorylatinmentioning

confidence: 99%

Rice GROWTH UNDER DROUGHT KINASE Is Required for Drought Tolerance and Grain Yield under Normal and Drought Stress Conditions

et al. 2014

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“…Homologues of RbcX are found in plants [43], so an obvious strategy for future research on the chloroplast Rubiscos is to see whether they can also be renatured in vitro by supplying cognate chaperonins and RbcX. Or could it be that even more chaperones are required to assemble the world's most important enzyme?…”

Section: (B) Rubisco Chaperonesmentioning

confidence: 99%

Assembly chaperones: a perspective

Ellis

2013

Phil. Trans. R. Soc. B

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The historical origins and current interpretation of the molecular chaperone concept are presented, with the emphasis on the distinction between folding chaperones and assembly chaperones. Definitions of some basic terms in this field are offered and misconceptions pointed out. Two examples of assembly chaperone are discussed in more detail: the role of numerous histone chaperones in fundamental nuclear processes and the co-operation of assembly chaperones with folding chaperones in the production of the world's most important enzyme.

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“…RBCX has a demonstrated essential Rubisco assembly function only in certain cyanobacteria (Onizuka et al, 2004), and its function in plants is unknown, although the Arabidopsis (Arabidopsis thaliana) RBCX proteins can increase the solubility of cyanobacterial LS when expressed in Escherichia coli (Kolesi nski et al, 2011). In maize, the two RBCX genes are both expressed in BS and M cells.…”

Section: Limitations To Rubisco Accumulation In Maizementioning

confidence: 99%

Ectopic Expression of Rubisco Subunits in Maize Mesophyll Cells Does Not Overcome Barriers to Cell Type-Specific Accumulation

et al. 2012

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In maize (Zea mays), Rubisco accumulates in bundle sheath but not mesophyll chloroplasts, but the mechanisms that underlie cell type-specific expression are poorly understood. To explore the coordinated expression of the chloroplast rbcL gene, which encodes the Rubisco large subunit (LS), and the two nuclear RBCS genes, which encode the small subunit (SS), RNA interference was used to reduce RBCS expression. This resulted in Rubisco deficiency and was correlated with translational repression of rbcL. Thus, as in C3 plants, LS synthesis depends on the presence of its assembly partner SS. To test the hypothesis that the previously documented transcriptional repression of RBCS in mesophyll cells is responsible for repressing LS synthesis in mesophyll chloroplasts, a ubiquitin promoter-driven RBCS gene was expressed in both bundle sheath and mesophyll cells. This did not lead to Rubisco accumulation in the mesophyll, suggesting that LS synthesis is impeded even in the presence of ectopic SS expression. To attempt to bypass this putative mechanism, a ubiquitin promoter-driven nuclear version of the rbcL gene was created, encoding an epitope-tagged LS that was expressed in the presence or absence of the Ubi-RBCS construct. Both transgenes were robustly expressed, and the tagged LS was readily incorporated into Rubisco complexes. However, neither immunolocalization nor biochemical approaches revealed significant accumulation of Rubisco in mesophyll cells, suggesting a continuing cell type-specific impairment of its assembly or stability. We conclude that additional cell type-specific factors limit Rubisco expression to bundle sheath chloroplasts.

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Initial characteristics of RbcX proteins from Arabidopsis thaliana

Cited by 29 publications

References 35 publications

Rice GROWTH UNDER DROUGHT KINASE Is Required for Drought Tolerance and Grain Yield under Normal and Drought Stress Conditions

Rice GROWTH UNDER DROUGHT KINASE Is Required for Drought Tolerance and Grain Yield under Normal and Drought Stress Conditions

Assembly chaperones: a perspective

Ectopic Expression of Rubisco Subunits in Maize Mesophyll Cells Does Not Overcome Barriers to Cell Type-Specific Accumulation

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