Initial Assessment of Lactate as Mediator of Exercise-Induced Retinal Protection

Abstract

Physical exercise is protective in rodent models of retinal injury and disease. Data suggest that this is in part mediated by brain-derived neurotrophic factor (BDNF) signal transduction. It has been hypothesized that exercised-induced neuroprotection may be mediated by increases in circulating lactate that in turn alter BDNF secretion. We therefore tested whether mice undergoing a treadmill running regimen previously shown to be protective in a mouse model of retinal degeneration (RD) have increased serum levels of lactate. Lactate levels in exercised and non-exercised mice were statistically indistinguishable. A role for circulating lactate in exercise-induced retinal protection is unsupported.

Electrophysiologic and Morphologic Strain Differences in a Low-Dose NaIO3-Induced Retinal Pigment Epithelium Damage Model

“…First, 5-µm paraffin sections were deparaffinized and rehydrated, as previously described. 31 Blocking buffer consisted of 0.1% (V/V) Tween-20 and 5% (V/V) donkey serum (#S1003-36 lot 16090957, US Biological) in 1× Tris-buffered saline (TBS, 46-012-CM, Corning, 1X solution composition: 20 mM Tris, 136 mM NaCl, pH approximately 7.4). Slides were incubated in blocking solution in a Coplin jar for 30 minutes and transferred to a humidified chamber.…”

Voluntary exercise modulates pathways associated with amelioration of retinal degenerative diseases

“…There is a strong link between exercise and the inflammatory system in the human body including in the CNS (Nieman and Wentz, 2019). Exercise is known to modulate key inflammatory mechanisms, mitigating and regulating systemic inflammation in neurological diseases such as multiple sclerosis (Dalgas et al, 2012), systemic lupus erythematous (Perandini et al, 2012), Alzheimer's and Parkinson's diseases (Fuller et al, 2020), and preclinical models of retinal degeneration (Crowston et al, 2017;Sellers et al, 2019;Zhang et al, 2019;Dantis Pereira de Campos et al, 2020). However, these antineuroinflammatory effects, whilst observed, have not been wellsubstantiated or often investigated past single target identification.…”