Initial angiogenic response in reduced renal mass after transplantation

Wilasrusmee, Chumpon; Botash, Robert; Silva, Monica Da; Shah, Gaurang; Siddiqui, Josephine; Bruch, David; Kittur, Smita; Wilasrusmee, Skuntala; Kittur, Dilip S.

doi:10.1016/s0022-4804(03)00346-9

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…Positive staining was mostly identified in the glomeruli and the peritubular capillaries, with sporadic distribution in the tubular epithelial cells. In agreement with Wilasrusmee et al [18], we observed that 1 week following the reduction in renal mass, VEGF expression was minimally induced in the glomerular and peritubular capillaries. On the other hand, other authors advocate the redistribution of VEGF in the basolateral aspect of renal tubular cells in the acute phase of ischemia/reperfusion injury [19].…”

Section: Discussionsupporting

confidence: 93%

Vascular Endothelial Growth Factor Protein Expression in a Renal Ablation Rabbit Model under Prolonged Warm and Cold Ischemia

Constantinides

Tyritzis

Evangelou³

et al. 2007

Am J Nephrol

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Background/Aims: To establish a potential correlation between renal and systemic production of vascular endothelial growth factor (VEGF) protein after prolonged ischemia in a renal ablation model under normothermic and hypothermic conditions. Methods: 38 uninephrectomized New Zealand rabbits were divided into 5 groups. The rabbits of each group underwent partial nephrectomy under 90 and 60 min of warm and 90 and 120 min of cold ischemia, except for the sham group (S), which served as control. Serum creatinine (SCr) and blood-urea-nitrogen (BUN) levels were assessed. On the 15th postoperative day (POD), the animals were euthanized and the remaining kidneys were evaluated. VEGF immunohistochemistry and serum Western blot analysis were performed. Results: In comparison to the control group, groups 60W, 90C and 120C showed 1.6-, 1.14- and 1.75-fold decreases, respectively, while the production of VEGF was significantly declined by 7.4-fold in group 90W (p < 0.05). Immunohistochemistry revealed prominent VEGF staining in the above-mentioned three groups, while in group 90W staining was negative. Serum biochemistry and microscopic evaluation verified the same differentiation. Conclusion: Renal and serum VEGF seem to have an analogous expression under conditions of prolonged ischemia. VEGF is overexpressed in hypothermic conditions compared to warm ischemia exceeding 60 min. Hypothermia can be more advantageous in a procedure applying prolonged ischemia.

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Section: Discussionsupporting

confidence: 93%

Vascular Endothelial Growth Factor Protein Expression in a Renal Ablation Rabbit Model under Prolonged Warm and Cold Ischemia

Constantinides

Tyritzis

Evangelou³

et al. 2007

Am J Nephrol

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“…Interestingly, Wilasrusmee et al (8) have supposed the angiogenic theory, as they demonstrated the up‐regulated expression of vascular endothelial growth factor (VEGF) and the increased histological glomerular density in association with the reduction of renal mass after transplantation in pigs. Beside VEGF, several other growth factors including insulin‐like growth factor‐I (IGF‐I) have been shown to induce glomerular hypertrophy (9, 10).…”

Section: Discussionmentioning

confidence: 99%

The morphological compensatory change of peritubular capillary network in chronic allograft rejection

Nishi

Imai

Alchi

et al. 2005

Clinical Transplantation

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To clarify the compensatory hemodynamic alterations in the interstitium of renal allograft biopsies with chronic rejection, we evaluated the morphological changes in the peritubular capillary (PTC) network. Seven renal biopsy specimens from recipients with chronic rejection presenting with elevation of serum creatinine of 1.9 +/- 0.5 mg/dL were examined. Renal biopsy specimens from their counterpart donors were used as normal controls. In each specimen, non-pathological interstitial areas without fibrosis, tubular atrophy or cell infiltration were compared with pathological areas (PA) showing fibrosis and/or tubular atrophy using a computer image analysis. Morphological measurements revealed that the mean cut surface area of the PTC in the non-pathological and pathological interstitial areas in the recipient biopsies were significantly larger than that of the normal controls (p < 0.001 and 0.001, respectively). In the recipient biopsies, both of the mean cut surface areas of the tubules and PTC in the non-pathological areas were significantly higher than those in the PA (p < 0.001). The mean glomerular diameter in the recipient biopsies was also significantly higher than that of the donors (p < 0.01). In this study, we provided pathological evidence for the compensatory interstitial and glomerular hemodynamic alterations in kidney graft with chronic rejection and the condition as single kidney.

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“…We determined the renal blood flow and volume of the remnant kidney using magnetic resonance imaging with phase contrast magnetic resonance angiography (MRI/PC MRA). Increased blood flow occurs in the arterial vasculature supplying the remnant kidney after the nephrectomy (18, 28). Finally, the histologic changes in the remnant kidney were characterized.…”

Section: Introductionmentioning

confidence: 99%

Vascular Endothelial Growth Factor-A, Matrix Metalloproteinase-1, and Macrophage Migration Inhibition Factor Changes in the Porcine Remnant Kidney Model: Evaluation by Magnetic Resonance Imaging

Misra

Glockner

2010

Journal of Vascular and Interventional Radiology

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Purpose To determine the expression of vascular endothelial growth factor-A (VEGF-A), macrophage migration inhibition factor (MIF), and matrix metalloproteinase-1 (MMP-1) in the porcine remnant kidney model and quantify renal blood flow and volume using phase contrast magnetic resonance imaging with magnetic resonance angiography (PC MRI/MRA). Material and methods In 23 pigs, the left renal artery was completely embolized using polyvinyl acrylide (PVA) particles and the right kidney partially embolized (remnant kidney) while six pigs served as controls. The animals were sacrificed early (day 3, 7, and 14, N=3), day 24 (D24, N=5), day 37 (D37, N=3), day 42 (D42, N=9), and day 84 (D84, N=3). MRI/PC MRA of the kidneys was performed prior to sacrifice. The remnant and control kidneys were harvested for Western blotting of VEGF-A, MMP-1, and MIF. Blood was removed for BUN and creatinine prior to embolization and at time of sacrifice. Results The kidney function after the embolization was characterized by chronic renal insufficiency. The renal artery blood flow, volume, and weight of the remnant kidney increased significantly over time when compared to controls. At early time points, there was increased expression of MIF and MMP-1 followed by an increase in the expression of VEGF-A by day 37 (P<0.05 when compared to control). Masson's trichrome staining of the remnant kidney revealed scarring in the tubulointerstitial space. Conclusions In this model, renal blood flow and volume increase as the remnant kidney hypertrophies and scars. There is increased expression of MIF, VEGF-A, and MMP-1 in the remnant kidney.

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Initial angiogenic response in reduced renal mass after transplantation

Cited by 4 publications

References 21 publications

Vascular Endothelial Growth Factor Protein Expression in a Renal Ablation Rabbit Model under Prolonged Warm and Cold Ischemia

Vascular Endothelial Growth Factor Protein Expression in a Renal Ablation Rabbit Model under Prolonged Warm and Cold Ischemia

The morphological compensatory change of peritubular capillary network in chronic allograft rejection

Vascular Endothelial Growth Factor-A, Matrix Metalloproteinase-1, and Macrophage Migration Inhibition Factor Changes in the Porcine Remnant Kidney Model: Evaluation by Magnetic Resonance Imaging

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