Inibidores de proteases de hospedeiros nativos e exóticos e sua ação em intestinos de lagartas de Thyrinteina leucoceraea

Marinho, Jeanne Scardini; Oliveira, M. G. A.; Guedes, R. N. C.; Pallini, Ângelo; Oliveira, Claudinei Lima

doi:10.1590/s0100-67622008000600018

Cited by 12 publications

(8 citation statements)

References 31 publications

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“…When detecting bacteria directly from plant extracts, false negatives are often reported as a result of contamination of DNA samples with inhibitors such as phenolic compounds (Khoodoo et al 2005). Guava leaves are also rich in phenolics (Marinho et al 2008) that interfere with DNA extraction and amplification. Our preliminary results from direct amplification using crude extracts from macerated guava leaves in water were not successful.…”

Section: Discussionmentioning

confidence: 99%

PCR-based methods for detection of Erwinia psidii on guava

Silva

Torres

Oliveira

et al. 2015

Trop. plant pathol.

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Erwinia psidii causes bacterial blight of guava (Psidium guajava), one of the most important diseases of this crop in Brazil. Control measures are not effective, and dissemination often occurs through contaminated but asymptomatic propagating plant material. Considering the need for a reliable and sensitive method for detecting the pathogen in asymptomatic plant material, E. psidii-specific PCR primers were designed from a 355-bp fragment of the recombinase A gene (recA) amplified from E. psidii type strain. Primer pair Ep2L/2R only amplified DNA from E. psidii and its detection limit was 10 −5 ng/μL of purified DNA and 10 CFU (colony forming units) of bacterial cell suspension/mL. Three methods, conventional PCR, IC-PCR, and BIO-PCR were evaluated with the selected primers for their potential to detect E. psidii on guava leaves. BIO-PCR and conventional PCR were more sensitive and less time-consuming than IC-PCR. The detection limits on extracts of macerated guava leaves spiked with bacterial suspensions at different concentrations were 10 and 10 3 CFU/mL for BIO-PCR and conventional PCR, respectively. The PCR method here described could be useful for developing a protocol for early detection of this pathogen in asymptomatic guava plants.

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Section: Discussionmentioning

confidence: 99%

PCR-based methods for detection of Erwinia psidii on guava

Silva

Torres

Oliveira

et al. 2015

Trop. plant pathol.

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“…Focusing on stored grain pests, it is known that several plant species produce proteins with insecticidal property, such as inhibitor of proteases (IPs) which play a key role in plant defense against various orders of insects (Marinho et al, 2008;Pereira et al, 2007). The insecticidal activity of IPs is due to inhibition of proteolytic enzymes in the midgut of insects, leading to malnutrition, delay in larvae development and even death (Mosolov and Valueva, 2008).…”

Section: Introductionmentioning

confidence: 99%

Trypsin (serine protease) inhibitors in peanut genotypes aiming for control of stored grain pests

Martins¹,

Santos²,

Filho³

et al. 2014

Afr. J. Agric. Res.

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Peanut seeds from different genotypes were evaluated for activities of trypsin inhibitors (serine protease), based on in vitro and in vivo assays, aiming to further selection of genitors in breeding programs for tolerance of stored grain pests. The in vitro assays were based on inhibition of insect digestive enzymes and also on thermal and pH stabilities of seed protein, while the in vivo assays were performed with insects Alphitobius diaperinus, Tribolium castaneum, Tenebrio molitor and Spodoptera frugiperda. Seed inhibitors of all genotypes inhibited bovine trypsin at 70 to 94%. The seed extract of BRS Havana inhibited T. castaneum and T. molitor up to 80% while the extract of BRS 151 L7 inhibited A. diapennus at nearly 20%. The seed inhibitors of both cultivars were stable at 80°C and also at different pH values. The two peanut genotypes are recommended as promising parents for breeding program aiming to selecting lines with tolerance to Tenebrio and Alphitobius insects.

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“…As a protective response to insect feeding, plants may produce proteinase inhibitors (PIs) (Ferreira et al 2005, Fortunato et al 2004, 2007. PIs are small proteins described as occurring in reproductive and vegetative tissues of more than 100 plant species (Ryan 1990, Fortunato et al 2004, Marinho et al 2008. The activity of PIs in the midgut of insect larvae causes amino acid deficiencies and, consequently, developmental arrest or mortality (Bazok et al 2005, Telang et al 2005, Vila et al 2005, Bayés et al 2006, Pilon et al 2006.…”

Section: Introductionmentioning

confidence: 99%

“…Ingestion of PIs does not eliminate proteolytic digestion in the midgut; instead, it results in the hyperproduction of proteolytic enzymes by the insect herbivore, which in turn will limit the availability of essential amino acids for protein synthesis, and consequently lead to reduced larval growth and development (Broadway & Duffey 1986, Ryan, 1990. However, herbivorous insects have the potential to become resistant to PIs (Broadway 1995, Vila et al 2005, Pilon et al 2006, Volpicella et al 2006, Marinho et al 2008, Moreira et al 2011.…”

Section: Introductionmentioning

confidence: 99%

“…The selection of appropriate inhibitors requires knowledge about the proteinases present in the insect midgut and the way they interact with different inhibitors. Serine proteinase activity has been found in a wide variety of lepidopteran pests (Broadway & Duffey 1986, Broadway 1995, Pompermayer et al 2001, Telang et al 2005, Pilon et al 2006, Volpicella et al 2006, including larvae of Thyrinteina (Marinho et al 2008(Marinho et al , 2011, an important eucalyptus defoliator in Brazil (Holtz et al 2003).…”

Section: Introductionmentioning

confidence: 99%

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Enzymatic Response of the Eucalypt Defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a Bis-Benzamidine Proteinase Inhibitor

et al. 2012

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Ingestion of proteinase inhibitors leads to hyperproduction of digestive proteinases, limiting the bioavailability of essential amino acids for protein synthesis, which affects insect growth and development. However, the effects of proteinase inhibitors on digestive enzymes can lead to an adaptive response by the insect. In here, we assessed the biochemical response of midgut proteinases from the eucalypt defoliator Thyrinteina arnobia (Stoll) to different concentrations of berenil, a bis-benzamidine proteinase inhibitor, on eucalyptus. Eucalyptus leaves were immersed in berenil solutions at different concentrations and fed to larvae of T. arnobia. Mortality was assessed daily. The proteolytic activity in the midgut of T. arnobia was assessed after feeding on plants sprayed with aqueous solutions of berenil, fed to fifth instars of T. arnobia for 48 h before midgut removal for enzymatic assays. Larvae of T. arnobia were able to overcome the effects of the lowest berenil concentrations by increasing their trypsin-like activity, but not as berenil concentration increased, despite the fact that the highest berenil concentration resulted in overproduction of trypsin-like proteinases. Berenil also prevented the increase of the cysteine proteinases activity in response to trypsin inhibition.

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Inibidores de proteases de hospedeiros nativos e exóticos e sua ação em intestinos de lagartas de Thyrinteina leucoceraea

Cited by 12 publications

References 31 publications

PCR-based methods for detection of Erwinia psidii on guava

PCR-based methods for detection of Erwinia psidii on guava

Trypsin (serine protease) inhibitors in peanut genotypes aiming for control of stored grain pests

Enzymatic Response of the Eucalypt Defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a Bis-Benzamidine Proteinase Inhibitor

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