Inï¿½vivo and inï¿½vitro induction of the apoptotic effects of oxysophoridine on colorectal cancer cells via the Bcl‑2/Bax/caspase-3 signaling pathway

Jin, Shaoju; Yang, Yun; Ma, Lei; Ma, Ben‐Hui; Ren, Liping; Guo, Lingyu; Wang, Wen‐Bao; Zhang, Yanxin; Zhi-gang, Zhao; Cui, Ming-chen

doi:10.3892/ol.2017.7227

Cited by 23 publications

(25 citation statements)

References 43 publications

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“…Ahmed & El Menoufy [45] and Farhadi et al [6], approved these mechanisms in executing tumor cells in vitro in human colon cancer cells and in human oral squamous cell carcinoma respectively. Also, our data are in accordance with Jin et al [46] who denoted a significant apoptotic effect on colorectal cancer cells in vitro and in vivo which was accompanied with the Bcl-2/Bax/caspase-3 signaling pathway. These observations were confirmed by the experiment applied by Russo et al [47] who approved these apoptotic mechanisms in human prostate cancer cells in vitro.…”

Section: Discussionsupporting

confidence: 92%

Secondary metabolites and Biological activities of Allium porrum L. attack Ehrlich ascites carcinoma in mice

Elhagrasi

Mahmoud

Foda³

et al. 2019

Egypt. J. Chem.

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A LLIUM vegetables have been recorded to be used in many medical remedies for protection and fighting diseases. The aim of the study is to investigate the phenolic compounds of methanol extract of Allium porrum L. aerial parts (MEAP) and introduce the methanolic extract as a candidate for the treatment of cancer in vivo as well as explore its antitumor mechanisms in attacking tumor cells at the molecular level. Isolation of phenolic and flavonoid compounds by column chromatography has been carried out. They were purified by Sephadex LH-20, and the structures were established with the aid of spectroscopic methods (UV, EI/MS, ESI/MS, 1 H-NMR, and 13 C-NMR spectroscopy). A total of 45 female Swiss albino mice were divided into five groups. One group represents the normal control. The four remaining groups were divided as follows: two groups were injected with Ehrlich ascites carcinoma (EAC) cells intraperitoneally and the other two were injected intramuscularly (to form solid tumor), representing the positive nontreated groups. One group from each model was administrated orally the extract at a dose of 300 mg/kg body weight 3 days weekly for 21 days after 24 h from the intraperitoneal or intramuscular injection of EAC cells. The influence of the extract on the apoptotic proteins were tested in EAC cells and in solid tumor of the treated groups compared with the nontreated ones. Eleven phenolic and flavonoid compounds were isolated from the aerial parts of methanol extract of A. porrum L., and characterized as follows: quercetin-3acid, and gallic acid. The gene expressions of caspase-3, Bax, and Bcl2 proteins and DNA fragmentation test were performed in the EAC cells. Hematological profile was conducted on mice's blood in addition to the Immuno-histochemical determination of P53 protein in solid tumors. MEAP has abundant phenolic compounds. It exhibits tumor suppressing mechanisms through the coordination between the apoptic proteins and DNA fragmentation in the EAC or solid cancer cells. MEAP is suggested to be a potent anticancer agent.

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Section: Discussionsupporting

confidence: 92%

Secondary metabolites and Biological activities of Allium porrum L. attack Ehrlich ascites carcinoma in mice

Elhagrasi

Mahmoud

Foda³

et al. 2019

Egypt. J. Chem.

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“…11 The caspases and Bcl-2 family of proteins (antiapoptotic and pro-apoptotic proteins) are recognized as the main regulators of the apoptotic pathway. 43 Bax translocation into mitochondria could change the cytochrome C permeability, followed by stimulation of the post-mitochondrial caspase, leading to apoptotic cell death. 43 Bcl-2 prevents cell apoptosis by decreasing the release of cytochrome C in order to suppress caspase-3 activation.…”

Section: Discussionmentioning

confidence: 99%

“…43 Bax translocation into mitochondria could change the cytochrome C permeability, followed by stimulation of the post-mitochondrial caspase, leading to apoptotic cell death. 43 Bcl-2 prevents cell apoptosis by decreasing the release of cytochrome C in order to suppress caspase-3 activation. 44 Intrinsic and extrinsic pathways are two main routes of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…8 It has been demonstrated that caspase-3 is a major effector in the apoptosis process and that its activation marks the irreversible stage of this pathway. 43 Caspase-3 is stimulated in the apoptotic cell both by intrinsic (mitochondrial) and extrinsic (death ligand) pathways. 44 It has been shown that TiO 2 NPs induced apoptosis in animal models by activating caspase-3.…”

Section: Discussionmentioning

confidence: 99%

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<p>NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study</p>

Abbasi-Oshaghi¹,

Mirzaei²,

Pourjafar³

2019

IJN

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Purpose This study evaluated the effects of titanium dioxide nanoparticles (TiO 2 NPs) on liver and intestine of normal rats. Methods Male rats were divided into four groups as follows: 1) control rats, 2) control rats that orally received 10 mg/kg TiO 2 NPs, 3) control rats that orally received 50 mg/kg TiO 2 NPs, and 4) control rats that orally received 100 mg/kg TiO 2 NPs. After 30 days, the NLRP3 inflammasome pathway (NLRP3, caspase-1, and IL-1β), antioxidant pathway (superoxide dismutase [SOD], glutathione peroxidase [GPx], and catalase [CAT]), inflammatory pathway (inducible nitric oxide synthase [iNOS] and tumor necrosis factor-α [TNF-α]), and the apoptosis pathway (p53, Bax, Bcl-2, and caspase-3) were determined in the intestine and liver of the rats. H&E and Masson’s trichrome (MT) staining as well as TUNEL assay were used to examine the liver and the intestine. Biochemical factors, cytotoxicity, ROS generation, and apoptosis rate were also determined in HepG2 and Caco-2 cells. Results TiO 2 NPs in a dose-dependent manner increased cytotoxicity, oxidative stress, and apoptosis rate in Caco-2 and HepG2 cells. The administration of TiO 2 NPs significantly reduced antioxidant enzyme activity and gene expressions (SOD, CAT, and GPx) as well as glutathione (GSH) levels and total antioxidant capacity (TAC) in a dose-dependent manner. TiO 2 NPs also induced the apoptosis pathway and inflammatory pathway gene expressions and caspase-3 activity in the intestine and liver. TUNEL assay was in agreement with gene expressions. TiO 2 NPs also led to morphological changes in the liver and intestine. Conclusion TiO 2 NPs could have cytotoxic effects on the intestine and liver structure and function by inducing oxidative stress, inflammation, and apoptosis.

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“…Caspase‐3 was the main terminal shear enzyme in the process of cell apoptosis. When the intracellular Bax/Bax homodimer increases, Caspase‐3 could be activated and transform into Cleaved‐caspase‐3, then to trigger the downstream cascade reaction, destroy the integrity of DNA and lead to cell apoptosis (Wang, Zhang, Wang, Song, & Yuan, ; Cong et al., ; Jin et al., ; Safhi, ). In this study, we found that GMP‐Na 2 (250 mg/kg) could down‐regulated the expressions of Caspase‐3, Bax mRNA and protein in liver tissue of mice with AHI, and upregulated the expression of Bcl‐2.…”

Section: Discussionmentioning

confidence: 99%

Disodium Guanylate Alleviates Acute Hepatic Injury Induced by Carbon Tetrachloride Via Antioxidative Stress and Antiapoptosis In Vivo and In Vitro

Jin

Liu

Wang

et al. 2019

Journal of Food Science

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Hepatic injury is one of the most common digestive system diseases worldwide in clinic. Guanylic acid or guanosine monophosphate (GMP) was an important component of nucleotides, which is mainly in the form of sodium salt (disodium guanylate, GMP‐Na2). However, its effect on hepatic injury has not yet been investigated. This study is to investigate the protective effects of GMP‐Na2 on acute hepatic injury induced by carbon tetrachloride (CCl4), and to explore its mechanism. The hepatic injury models of mice and HL‐7702 cells were induced by CCl4. The alanine transaminase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px), malondialdehyde (MDA), total antioxidant capacity (T‐AOC) were determined by biochemical method. Hematoxylin–eosin staining were used to determine the morphological changes on liver tissue in mice. The mRNA and protein expressions of caspase‐3, Bax, and Bcl‐2 were detected by RT‐PCR and Western blot analysis. Our results show that GMP‐Na2 treatment significantly decreased the activities of ALT and AST, and the levels of MDA as well as increased the levels of SOD, GSH‐Px, and T‐AOC. Importantly, GMP‐Na2 effectively enhanced the antiapoptosis function by upregulating Bcl‐2 expression and downregulating caspase‐3 and Bax expressions in vivo and in vitro. Moreover, the histopathological changes of liver tissue were obviously improved after GMP‐Na2 treatment. These findings suggest that GMP‐Na2 has protective effects on hepatic injury, and its mechanisms may be associated with antioxidative stress and antiapoptosis.

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Inï¿½vivo and inï¿½vitro induction of the apoptotic effects of oxysophoridine on colorectal cancer cells via the Bcl‑2/Bax/caspase-3 signaling pathway

Cited by 23 publications

References 43 publications

Secondary metabolites and Biological activities of Allium porrum L. attack Ehrlich ascites carcinoma in mice

Secondary metabolites and Biological activities of Allium porrum L. attack Ehrlich ascites carcinoma in mice

<p>NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study</p>

Disodium Guanylate Alleviates Acute Hepatic Injury Induced by Carbon Tetrachloride Via Antioxidative Stress and Antiapoptosis In Vivo and In Vitro

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