Inhibitory role of oleanolic acid and esculetin in HeLa cells involve multiple signaling pathways

Edathara, Prajitha Mohandas; Chintalapally, Shivakanth; Makani, Venkata Krishna Kanth; Pant, Chitrakshi; Yarlagadda, Suresh; Rao, Manohar D.; Bhadra, Manika Pal

doi:10.1016/j.gene.2020.145370

Cited by 21 publications

(16 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have shown that OA and its derivatives have no cytotoxicity to normal human cells [14] and it inhibits tumor development in various malignant tumors through multiple methods and pathways. In vivo experiments showed that OA signi cantly reduced the size and quality of tumors, and in vitro experiments revealed that OA has a signi cant inhibitory effect on cell migration, cell viability and proliferation ability [15,16] . OA has a sensitizing effect on radiotherapy of C6 cell-bearing rats [17] .…”

Section: Discussionmentioning

confidence: 99%

Oleanolic acid mediated the proliferation and invasion of U251 glioma cells and promoted their apoptosis through the IKK-β, MAPK3, and MAPK4 signaling pathway

Huang

Lin

Feng

et al. 2021

Preprint

View full text Add to dashboard Cite

ObjectTo investigate the effects of Oleanolic acid (OA) on proliferation, apoptosis, migration, and invasion of human glioma cell U251, as well as IKK-β and MAPK signaling pathways.MethodsThe binding of OA to IKK-β and MAPK signaling pathway essential proteins IKK-β, MAPK3, and MAPK4 was analyzed by molecular docking technique. U251 cells were treated with different concentrations of OA. The proliferation and apoptosis rates of U251 cells were detected by CCK-8 assay, MTT assay, cell cloning assay, and AnnexinⅤ FITC/PI double staining assay. Transwell chamber assay was used to detect migration and invasion of U251 cells. Finally, Western blotting was used to detect the protein expression levels of IKK-β, MAPK3, and MAPK4 in U251 cells treated with OA.ResultsThe results of molecular docking showed that OA could stably bind to IKK-β, MAPK3, and MAPK4 proteins. OA could not only effectively inhibit the proliferation and induce apoptosis of U251 cells (P < 0.05), but also significantly inhibit the invasion of U251 cells (P < 0.05). Western blot assay confirmed that OA could dramatically inhibit the protein expression levels of IKK-β, MAPK3, and MAPK4 in U251 cells (P < 0.05).ConclusionsOA may inhibit the proliferation, migration, and invasion of glioma U251 cells by binding key molecules of the IKK-β signaling pathway and essential target proteins of MAPK3 and MAPK4 in the MAPK signaling pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Oleanolic acid mediated the proliferation and invasion of U251 glioma cells and promoted their apoptosis through the IKK-β, MAPK3, and MAPK4 signaling pathway

Huang

Lin

Feng

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Studies have shown that OA and its derivatives have no cytotoxicity to normal human cells [16] and it inhibits tumor development in various malignant tumors through multiple methods and pathways. In vivo experiments showed that OA signi cantly reduced the size and quality of tumors, and in vitro experiments revealed that OA has a signi cant inhibitory effect on cell migration, cell viability and proliferation ability [17,18] . OA has a sensitizing effect on radiotherapy of C6 cell-bearing rats [19] .…”

Section: Discussionmentioning

confidence: 99%

Oleanolic Acid Inhibits the Proliferation and Invasion of U251Glioma Cells and Promotes their Apoptosis through the IKK-β, MAPK3, and MAPK4 Signaling Pathway

Huang

Lin

Feng

et al. 2021

Preprint

View full text Add to dashboard Cite

Object: To investigate the effects of Oleanolic acid (OA) on proliferation, apoptosis, migration, and invasion of human glioma cell U251, as well as IKK-β and MAPK signaling pathways.Methods: The binding of OA to IKK-β and MAPK signaling pathway essential proteins IKK-β, MAPK3, and MAPK4 was analyzed by molecular docking technique. U251 cells were treated with different concentrations of OA. The proliferation and apoptosis rates of U251 cells were detected by CCK-8 assay, MTT assay, cell cloning assay, and AnnexinⅤ FITC/PI double staining assay. Transwell chamber assay was used to detect migration and invasion of U251 cells. Western blot was used to detect and analyze the expression levels of CYP17A1, IKK-β, PTGS2 and MAPK3/4 protein in U251 cells after OA treatment. Finally, the transcriptome sequencing method was used to detect the differentially expressed genes in the two groups, and the GO and KEGG enrichment analysis were performed.Results: The results of molecular docking showed that OA could stably bind to IKK-β, MAPK3, and MAPK4 proteins. OA could not only effectively inhibit the proliferation and induce apoptosis of U251 cells (P < 0.05), but also significantly inhibit the invasion of U251 cells (P < 0.005). Western blot assay confirmed that OA could dramatically inhibit the protein expression levels of CYP17A1, IKK-β, PTGS2, MAPK3, and MAPK4 in U251 cells (P < 0.01). A total of 446 significantly differentially expressed genes were detected in transcriptome sequencing, of which 96 were up-regulated genes and 350 were down-regulated genes. These genes are mainly involved in processes such as inflammation, metabolism, immunity, and regulation of cell growth.Conclusions: OA may inhibit the proliferation, migration, and invasion of glioma U251 cells by binding key molecules of the IKK-β signaling pathway and essential target proteins of MAPK3 and MAPK4 in the MAPK signaling pathway.

show abstract

“…Betulinic acid showed significant PTP1B inhibitory activity, with IC 50 values of 3.5 μM [24]. [54] Anti-hypertension (in vivo) [55] Anti-tumor (in vitro) [56,57] Neuroprotection(in vitro) [58] Anti-cholesterol(in vitro) […”

Section: Lupane Triterpenoidsmentioning

confidence: 99%

“…It was found from callus tissue from the stems of A. arguta , together with 2 α ,3 β -dihydroxyolean-12-en-28-oic acid 78 [ 26 ]. Oleanolic acid 77 is abundant in nature and exhibits a wide range of biological activities including anti-inflammatory [ 54 ], anti-hypertension [ 55 ], anti-tumor [ 56 , 57 ], neuroprotection [ 58 ], and anti-cholesterol activities [ 59 ]. Oleanolic acid was performed to test the effect on apoptosis and autophagy of SMMC-7721 Hepatoma cells.…”

Section: Chemical Constituentsmentioning

confidence: 99%

“… Part Bioactivity Refs. 77 Oleanolic acid A. arguta [ 26 ] Stems Anti-inflammatory (in vitro) [ 54 ] Anti-hypertension (in vivo) [ 55 ] Anti-tumor (in vitro) [ 56 , 57 ] Neuroprotection(in vitro) [ 58 ] Anti-cholesterol(in vitro) [ 59 ] 78 2 α ,3 β -Dihydroxyolean-12-en-28-oic acid A. arguta [ 26 ] Stems 79 2 α ,3 α -Dihydroxyolean-12-en-28-oic acid A. chinensis Planch [ 63 ] Fruits 80 2 α ,3 β ,23-Trihydroxyolean-12-en-28-oic acid A. deliciosa [ 31 ] Peels Antifungal (in vitro) [ 31 ] 81 2 α ,3 α ,24-Trihydroxyolean-12-en-28-oic acid A. deliciosa [ 31 ] Peels Antifungal (in vitro) [ 31 ] 82 3 β -O-Acetyloleanolic acid A. arguta [ 64 ] A. chinensis [ 45 ] Stems Roots Anti-angiogenesis (in vitro) [ 45 ] 83 …”

Section: Chemical Constituentsmentioning

confidence: 99%

See 1 more Smart Citation

Advances in Research on Chemical Constituents and Their Biological Activities of the Genus Actinidia

Liu

et al. 2021

Nat. Prod. Bioprospect.

View full text Add to dashboard Cite

Kiwi, a fruit from plants of the genus Actinidia, is one of the famous fruits with thousand years of edible history. In the past twenty years, a great deal of research has been done on the chemical constituents of the Actinidia species. A large number of secondary metabolites including triterpenoids, flavonoids, phenols, etc. have been identified from differents parts of Actinidia plants, which exhibited significant in vitro and in vivo pharmacological activities including anticancer, anti-inflammatory, neuroprotective, anti-oxidative, anti-bacterial, and anti-diabetic activities. In order to fully understand the chemical components and biological activities of Actinidia plants, and to improve their further research, development and utilization, this review summarizes the compounds extracted from different parts of Actinidia plants since 1959 to 2020, classifies the types of constituents, reports on the pharmacological activities of relative compounds and medicinal potentials.

show abstract

Inhibitory role of oleanolic acid and esculetin in HeLa cells involve multiple signaling pathways

Cited by 21 publications

References 22 publications

Oleanolic acid mediated the proliferation and invasion of U251 glioma cells and promoted their apoptosis through the IKK-β, MAPK3, and MAPK4 signaling pathway

Oleanolic acid mediated the proliferation and invasion of U251 glioma cells and promoted their apoptosis through the IKK-β, MAPK3, and MAPK4 signaling pathway

Oleanolic Acid Inhibits the Proliferation and Invasion of U251Glioma Cells and Promotes their Apoptosis through the IKK-β, MAPK3, and MAPK4 Signaling Pathway

Advances in Research on Chemical Constituents and Their Biological Activities of the Genus Actinidia

Contact Info

Product

Resources

About