Inhibitory receptors sensing HLA-G1 molecules in pregnancy: Decidua-associated natural killer cells express LIR-1 and CD94/NKG2A and acquire p49, an HLA-G1-specific receptor

Ponte, Marco; Cantoni, Claudia; Biassoni, Roberto; Tradori-Cappai, Andrea; Bentivoglio, G; Vitale, Chiara; Bertone, Stefania; Moretta, Alessandro; Moretta, Lorenzo; Mingari, Maria Cristina

doi:10.1073/pnas.96.10.5674

Cited by 337 publications

(253 citation statements)

References 41 publications

Supporting

Mentioning

247

Contrasting

Order By: Relevance

“…As with other MHC class I molecules, HLA-G is expressed on the cell surface complexed with ␤ 2 -microglobulin and interacts with a variety of receptors on cells of the innate immune system, including NK cells and monocytes (7)(8)(9)(10)(11). One of the intriguing characteristics of the molecular biology of HLA-G is the expression of an alternatively spliced transcript coding for a truncated, soluble molecule (12,13), now designated HLA-G5.…”

Section: T He Nonclassical Mhc Class I Molecule Hla-g Has Beenmentioning

confidence: 99%

A Soluble Isoform of the Rhesus Monkey Nonclassical MHC Class I Molecule Mamu-AG Is Expressed in the Placenta and the Testis

Ryan¹,

Grendell²,

Geraghty

et al. 2002

The Journal of Immunology

View full text Add to dashboard Cite

The nonclassical MHC class I locus HLA-G is expressed primarily in the placenta, although other sites of expression have been noted in normal and pathological situations. In addition, soluble HLA-G isoforms have been detected in the serum of pregnant and nonpregnant women as well as men. The rhesus monkey placenta expresses a novel nonclassical MHC class I molecule Mamu-AG, which has features remarkably similar to those of HLA-G. We determined that the rhesus placenta expresses Mamu-AG mRNA (Mamu-AG5), retaining intron 4 as previously noted in HLA-G5. Immunostaining experiments with Ab 16G1 against the soluble HLA-G5 intron 4 peptide demonstrated that an immunoreactive protein(s) was present in the syncytiotrophoblasts of the chorionic villi of the rhesus placenta, within villous cytotrophoblasts, and occasionally within cells of the villous stroma. The Mamu-AG5 mRNA was readily detected in rhesus testis (although not in ejaculated sperm). Whereas an Ab against membrane-bound Mamu-AG stained few cells, primarily in the interstitium of the testis, there was consistent immunostaining for Mamu-AG5 in cells within the seminiferous tubules, which was corroborated by localization of Mamu-AG mRNA by in situ hybridization. While primary spermatocytes were negative, Sertoli cells, spermatocytes, and spermatids were consistently positive for 16G1 immunostaining. The specific recognition of the soluble Mamu-AG isoform was confirmed by Western blotting of Mamu-AG5 expressed in heterologous cells. The results demonstrate that a soluble nonclassical MHC class I molecule is expressed in the rhesus monkey placenta and testis, and confirm and extend the unique homology between HLA-G and the rhesus nonclassical molecule Mamu-AG.

show abstract

Section: T He Nonclassical Mhc Class I Molecule Hla-g Has Beenmentioning

confidence: 99%

A Soluble Isoform of the Rhesus Monkey Nonclassical MHC Class I Molecule Mamu-AG Is Expressed in the Placenta and the Testis

Ryan¹,

Grendell²,

Geraghty

et al. 2002

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…[26][27][28][29][30][31][32] KIR2DL4 binds trophoblast-specific HLA-G and regulates vascular growth in the decidua that facilitates successful pregnancy. 33,34 Ligands for KIR2DL5, KIR3DL3 or any activating KIR (aKIR) have not been identified.…”

Section: Introductionmentioning

confidence: 99%

Combination of KIR and HLA gene variants augments the risk of developing birdshot chorioretinopathy in HLA-A*29-positive individuals

Levinson

Luo

et al. 2008

Genes Immun

View full text Add to dashboard Cite

“…27 Although both receptors have other HLA class I ligands, their highest affinity is for HLA-G. 28 KIR2DL4/p49 (CD158d) was also described as an HLA-G-specific receptor expressed by all NK cells. 29,30 Following our previous studies that described HLA-G expression in tumor lesions, such as melanomas, 5,31,32 we analyzed here the mechanisms by which both expression and function of HLA-G are regulated in malignant cells.…”

mentioning

confidence: 99%

Switch ofHLA-G alternative splicing in a melanoma cell line causes loss of HLA-G1 expression and sensitivity to NK lysis

et al. 2005

View full text Add to dashboard Cite

these points by studying a melanoma cell line derived from a surgically-removed HLA-G-positive melanoma lesion. We show that HLA-G expression in melanoma cells can be regulated at the mRNA splicing level. Indeed, melanoma cells rapidly switched from cell-surface HLA-G1 to intra-cellular HLA-G2 expression. This mechanism restored tumor sensitivity to NK lysis. Moreover, switch from HLA-G1 to HLA-G2 was strong enough to prevent re-expression of immunoprotective HLA-G1 even following treatments with cytokines and DNA demethylating agent. Modulating HLA-G at the mRNA splicing level would be an efficient way of lifting in vivo HLA-G-mediated tumor immune escape. ' 2005 Wiley-Liss, Inc.

show abstract

Inhibitory receptors sensing HLA-G1 molecules in pregnancy: Decidua-associated natural killer cells express LIR-1 and CD94/NKG2A and acquire p49, an HLA-G1-specific receptor

Cited by 337 publications

References 41 publications

A Soluble Isoform of the Rhesus Monkey Nonclassical MHC Class I Molecule Mamu-AG Is Expressed in the Placenta and the Testis

A Soluble Isoform of the Rhesus Monkey Nonclassical MHC Class I Molecule Mamu-AG Is Expressed in the Placenta and the Testis

Combination of KIR and HLA gene variants augments the risk of developing birdshot chorioretinopathy in HLA-A*29-positive individuals

Switch ofHLA-G alternative splicing in a melanoma cell line causes loss of HLA-G1 expression and sensitivity to NK lysis

Contact Info

Product

Resources

About