Inhibitory Potential of Five Traditionally Used Native Antidiabetic Medicinal Plants onα-Amylase,α-Glucosidase, Glucose Entrapment, and Amylolysis KineticsIn Vitro

Abstract

Five traditionally used antidiabetic native medicinal plants of Mauritius, namely, Stillingia lineata (SL), Faujasiopsis flexuosa (FF), Erythroxylum laurifolium (EL), Elaeodendron orientale (EO), and Antidesma madagascariensis (AM), were studied for possible α-amylase and α-glucosidase inhibitory property, glucose entrapment, and amylolysis kinetics in vitro. Only methanolic extracts of EL, EO, and AM (7472.92 ± 5.99, 1745.58 ± 31.66, and 2222.96 ± 13.69 μg/mL, resp.) were found to significantly (P < 0.05) inhibit α-amylase and were comparable to acarbose. EL, EO, AM, and SL extracts (5000 μg/mL) were found to significantly (P < 0.05) inhibit α-glucosidase (between 87.41 ± 3.31 and 96.87 ± 1.37% inhibition). Enzyme kinetic studies showed an uncompetitive and mixed type of inhibition. Extracts showed significant (P < 0.05) glucose entrapment capacities (8 to 29% glucose diffusion retardation index (GDRI)), with SL being more active (29% GDRI) and showing concentration-dependent activity (29, 26, 21, 14, and 5%, resp.). Amylolysis kinetic studies showed that methanolic extracts were more potent inhibitors of α-amylase compared to aqueous extracts and possessed glucose entrapment properties. Our findings tend to provide justification for the hypoglycaemic action of these medicinal plants which has opened novel avenues for the development of new phytopharmaceuticals geared towards diabetes management.

Antidiabetic Activity of Endophytic Fungi Isolated From Ficus Religiosa

“…2. It was observed that at lower concentration crude extract showed maximum inhibition activity with (42% ± 0.01) percentage [6].…”

“…The inhibition by natural products is safer than synthetic drug. The synthetic drugs cause more side effects than the natural products [6]. F. religiosa is a traditional medicinal plant known for its antifungal antidiabetic, antiasthmatic, and anticancer activity [13].…”

“…It managed to prevent efflux of glucose. It could make effective agent for controlling diabetes [6,7].…”

“…The glucose movement from internal solution to external solution (beaker solution) was measured every ½ hr by DNSA method. Three replications were done for every ½ hr for 3 hrs [6].…”

The Effect of the Extracts of Endophytic Fungi on Pancreatic α-Amylase Activity

“…Determination of α-amylase activity was carried out by the modified method used for measurement in the plant extract [13]. Solution of starch as a substrate was prepared in concentration 1 g 10 mL -1 water, boiled for 2 minutes, adjusted to 100 mL with distilled water and used within 2-3 days.…”