Inhibitory of EV-A71 virus-induced apoptosis by ZVAD through ROS mediated signaling pathways

Xu, Tiantian; Ren, Zheng; CHEN, DANYANG; CHEN, HAIYANG; Zhao, Mingqi; Guo, Min; Chen, Yi; Wang, Chang-bing; Kuang, Lu; Li, Yinghua; Zhu, Bing

doi:10.32604/biocell.2022.017757

Cited by 3 publications

(1 citation statement)

References 35 publications

(41 reference statements)

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“…Each protein was quantified by a BCA assay. X-ray film was used to monitor the target protein (Mishra et al, 2017;Xu et al, 2022).…”

Section: Western Blottingmentioning

confidence: 99%

Quercetin induced HepG2 cells apoptosis through ATM/JNK/STAT3 signaling pathways

LIU¹,

CHEN²,

Su³

et al. 2023

Biocell

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Liver cancer is the seventh most common malignant tumor in the world and is the second highest cause of death due to cancer. Quercetin, a flavonoid with low toxicity, widely exists in various fruits and vegetables. It has the potential to be a therapeutic agent against various cancers. This study aimed to demonstrate the anti-tumor effect of quercetin on HepG2 cells. Quercetin suppressed the HepG2 cell proliferation in a dose-dependent manner in cell viability assay. Induction of cell apoptosis was confirmed by apoptotic cells population (sub-G1 peak) detected by flow cytometer. A decrease in mitochondrial membrane potential and caspase-3 activation were also demonstrated in this study. Furthermore, quercetin induced HepG2 cell apoptosis through ROS-mediated phosphorylated ataxia-telangiectasia mutated, c-Jun Nterminal kinases, signal transducer, and activator of transcription 3 (STAT-3), and Bax signaling pathways. These resultssuggest that quercetin has the potential to become an effective drug against the tumor.

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“…Each protein was quantified by a BCA assay. X-ray film was used to monitor the target protein (Mishra et al, 2017;Xu et al, 2022).…”

Section: Western Blottingmentioning

confidence: 99%

Quercetin induced HepG2 cells apoptosis through ATM/JNK/STAT3 signaling pathways

LIU¹,

CHEN²,

Su³

et al. 2023

Biocell

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Ebselen inhibits enterovirus A71-induced apoptosis through reactive oxygen species-mediated signaling pathway

Chen

Ning²,

Liu³

et al. 2023

Mol Biol Rep

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Epigallocatechin-3-gallate Induced HepG2 Cells Apoptosis through ROSmediated AKT /JNK and p53 Signaling Pathway

Guan

et al. 2023

CCDT

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Background: Hepatocarcinoma is the third leading cause of cancer-related deaths around the world. Recently, some studies have reported that Epigallocatechin-3-gallate (EGCG) may have the potential for anti-cancer. However, the affection and putative mechanisms of cytotoxicity induced by EGCG in HepG2 cells remain unknown. Based on the above, the present study evaluated the effect of EGCG on the cytotoxic and anti-cancer mechanisms on HepG2 cells. Methods: The effect of EGCG on the apoptosis of Hep-G2 cells and its mechanism were studied by cell counting kit-8, mitochondrial membrane potential assay with JC-1, Annexin V-FITC apoptosis detection, cell cycle, and apoptosis analysis, one step TUNEL apoptosis assay, caspase 3 activity assay, caspase 9 activity Assay, Reactive Oxygen Species assay, and Western blot. Results: EGCG-induced HepG2 cell apoptosis was confirmed by accumulation of the sub-G1 cells population, translocation of phosphatidylserine, depletion of mitochondrial membrane potential, DNA fragmentation, caspase-3 activation, caspase-9 activation, and poly (ADP-ribose) polymerase cleavage. Furthermore, EGCG enhanced cytotoxic effects on HepG2 cells and triggered intracellular reactive oxygen species; the signaling pathways of AKT, JNK, and p53 were activated to advance cell apoptosis. Conclusion: The results reveal that EGCG may provide useful information on EGCG-induced HepG2 cell apoptosis and be an appropriate candidate for cancer chemotherapy.

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Inhibitory of EV-A71 virus-induced apoptosis by ZVAD through ROS mediated signaling pathways

Cited by 3 publications

References 35 publications

Quercetin induced HepG2 cells apoptosis through ATM/JNK/STAT3 signaling pathways

Quercetin induced HepG2 cells apoptosis through ATM/JNK/STAT3 signaling pathways

Ebselen inhibits enterovirus A71-induced apoptosis through reactive oxygen species-mediated signaling pathway

Epigallocatechin-3-gallate Induced HepG2 Cells Apoptosis through ROSmediated AKT /JNK and p53 Signaling Pathway

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