Inhibitory Mechanism of Store-operated Ca2+ Channels by Zinc

Gore, Ariel; Moran, Arie; Hershfinkel, Michal; Sekler, Israel

doi:10.1074/jbc.m400005200

Cited by 42 publications

(28 citation statements)

References 56 publications

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“…Furthermore, the membrane is believed to be impermeable to polyvalent anions such as sulphate, citrate and phosphate, based on the fact that the vesicles are stable in 1 mol l -1 solutions of these anions. The same study also revealed that zinc ions at concentrations from 25 to 50 mmol l -1 inhibited rupture (Luchtel et al, 1991), which suggests that calcium channels may be important for the rupture of mucin vesicles, as zinc is known to inhibit some calcium channels (Büsellberg et al, 1991;Gore et al, 2004). Furosemide, a Na…”

Section: Introductionmentioning

confidence: 90%

Stabilization and swelling of hagfish slime mucin vesicles

Herr

Winegard

O’Donnell

et al. 2010

Journal of Experimental Biology

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SUMMARYWhen agitated, Atlantic hagfish (Myxine glutinosa) produce large quantities of slime that consists of hydrated bundles of protein filaments and membrane-bound mucin vesicles from numerous slime glands. When the slime exudate contacts seawater, the thread bundles unravel and the mucin vesicles swell and rupture. Little is known about the mechanisms of vesicle rupture in seawater and stabilization within the gland, although it is believed that the vesicle membrane is permeable to most ions except polyvalent anions. We hypothesized that the most abundant compounds within the slime gland exudate have a stabilizing effect on the mucin vesicles. To test this hypothesis, we measured the chemical composition of the fluid component of hagfish slime exudate and conducted functional assays with these solutes to test their ability to keep the vesicles in a condensed state. We found K + concentrations that were elevated relative to plasma, and Na + , Cl -and Ca 2+ concentrations that were considerably lower. Our analysis also revealed high levels of methylamines such as trimethylamine oxide (TMAO), betaine and dimethylglycine, which had a combined concentration of 388 mmol l -1 in the glandular fluid. In vitro rupture assays demonstrated that both TMAO and betaine had a significant effect on rupture, but neither was capable of completely abolishing mucin swelling and rupture, even at high concentrations. This suggests that some other mechanism such as the chemical microenvironment within gland mucous cells, or hydrostatic pressure is responsible for stabilization of the vesicles within the gland.

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Section: Introductionmentioning

confidence: 90%

Stabilization and swelling of hagfish slime mucin vesicles

Herr

Winegard

O’Donnell

et al. 2010

Journal of Experimental Biology

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“…Store operated calcium channels in the plasma membranes of salivary cells are inhibited by extracellular zinc binding at a site distinct from the channel [106]. In airway epithelial cells exposure to extracellular zinc causes an increase in [Ca 2+ ] cyt by its interaction with the P2X receptor channel [107].…”

Section: Zincmentioning

confidence: 99%

Cell signaling, beyond cytosolic calcium in eukaryotes

Permyakov

Kretsinger

2009

Journal of Inorganic Biochemistry

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“…A functional link has been proposed between L-type calcium channels and the zinc efflux transporter ZnT1 (Ohana et al, 2006;Segal et al, 2004), and extracellular zinc can trigger the release of calcium from intracellular pools in HT29 cells (Hershfinkel et al, 2001) and inhibit calcium influx in several cultured cell lines (Gore et al, 2004). A dramatic increase in calcium occurs in embryos from zinc-deficient Zip2-knockout mice (Peters et al, 2007).…”

Section: Essential Metal Interactionsmentioning

confidence: 98%

The genetics of essential metal homeostasis during development

Kambe

Weaver

Andrews

2008

Genesis

118

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The essential metals copper, zinc, and iron play key roles in embryonic, fetal, and postnatal development in higher eukaryotes. Recent advances in our understanding of the molecules involved in the intricate control of the homeostasis of these metals and the availability of natural mutations and targeted mutations in many of the genes involved have allowed for elucidation of the diverse roles of these metals during development. Evidence suggests that the ability of the embryo to control the homeostasis of these metals becomes essential at the blastocyst stage and during early morphogenesis. However, these metals play unique roles throughout development and exert pleiotropic, metal-specific, and often cell-specific effects on morphogenesis, growth, and differentiation. Herein, we briefly review the major players known to be involved in the homeostasis of each of these essential metals and their known roles in development.

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Inhibitory Mechanism of Store-operated Ca2+ Channels by Zinc

Cited by 42 publications

References 56 publications

Stabilization and swelling of hagfish slime mucin vesicles

Stabilization and swelling of hagfish slime mucin vesicles

Cell signaling, beyond cytosolic calcium in eukaryotes

The genetics of essential metal homeostasis during development

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