Inhibitory efficacy of Lactococcus lactis on food born pathogens and food spoilage bacteria

Mirdamadi, Saeed; Ghazvini, Shadi Agha; Ehsani, Morteza; Falahpour, M; Tafreshi, Say-yed Hesameddin

doi:10.1016/j.jbiotec.2008.07.098

Cited by 2 publications

(8 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This culture served as the inoculums for the susceptibility studies, starting with approximately 10 6 CFU/mL in the test tubes. These CFU counts were accurately and reproducibly obtained by inoculation of 0.1 mL of the culture having an absorbance value of 0.2 as determined by optical density measurement at 600 nm using a UV/VIS spectrophotometer (Spectronic 20 D, USA) [10,11].…”

Section: Microbial Assaysmentioning

confidence: 99%

“…First, nisin stock solution was prepared using Nisaplin as the source at concentration of 1000 IU/mL (equivalent to 10 mg/mL) in sterile water [11]. Then for quantification, standard solutions of purified nisin with concentrations ranging from 0 up to 1000 IU/mL were prepared.…”

Section: Standard Calibration Curve Of Nisinmentioning

confidence: 99%

“…M. luteus ATCC 10240 was used as the indicator organism. This organism is the most sensitive to nisin and chosen based on the reference [11]. After incubation at 37°C for 24 h, the diameters of the inhibition zone were recorded, and nisin standard calibration curve obtained [11].…”

Section: Standard Calibration Curve Of Nisinmentioning

confidence: 99%

“…After comparison with the standard calibration curve of nisin, the strength (inhibition zone) of the nisin-loaded nanoparticles was calculated and compared with that of free nisin [11]. [11].…”

Section: Activity Assay Of Nisin-loaded Nanoparticles With Agar Diffumentioning

confidence: 99%

“…Wan et al [18] studied the incorporation of nisin in micro particles of calcium alginate and obtained an incorporation efficiency of 87-93% besides the loaded form was 100% active against an indicator culture of Lactobacillus curvatus both in MRS broth and reconstituted skim milk. Mirdamadi et al studied the effect of liposomes containing nisin and a culture producing nisin against L. monocytogenes (ATCC 19117), S. aureus (ATCC 25923) and Eshershia coli (ATCC 25922) and found that nisinloaded liposomes were more effective than free nisin [11]. The results of others showed that encapsulation of nisin can increase antimicrobial properties of nisin and prevent from unwilling interactions with other ingredient in food materials.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A Comparative Study Between the Antibacterial Effect of Nisin and Nisin-Loaded Chitosan/Alginate Nanoparticles on the Growth of Staphylococcus aureus in Raw and Pasteurized Milk Samples

Zohri

Alavidjeh

Haririan

et al. 2010

Probiotics & Antimicro. Prot.

112

View full text Add to dashboard Cite

The aim of this study was to evaluate the antibacterial effect of nisin-loaded chitosan/alginate nanoparticles as a novel antibacterial delivery vehicle. The nisin-loaded nanoparticles were prepared using colloidal dispersion of the chitosan/alginate polymers in the presence of nisin. After the preparation of the nisin-loaded nanoparticles, their physicochemical properties such as size, shape, and zeta potential of the formulations were studied using scanning electron microscope and nanosizer instruments, consecutively. FTIR and differential scanning calorimetery studies were performed to investigate polymer-polymer or polymer-protein interactions. Next, the release kinetics and entrapment efficiency of the nisin-loaded nanoparticles were examined to assess the application potential of these formulations as a candidate vector. For measuring the antibacterial activity of the nisin-loaded nanoparticles, agar diffusion and MIC methods were employed. The samples under investigation for total microbial counts were pasteurized and raw milks each of which contained the nisin-loaded nanoparticles and inoculated Staphylococcus aureus (ATCC 19117 at 10(6) CFU/mL), pasteurized and raw milks each included free nisin and S. aureus (10(6) CFU/mL), and pasteurized and raw milks each had S. aureus (10(6) CFU/mL) in as control. Total counts of S. aureus were measured after 24 and 48 h for the pasteurized milk samples and after the time intervals of 0, 6, 10, 14, 18, and 24 h for the raw milk samples, respectively. According to the results, entrapment efficiency of nisin inside of the nanoparticles was about 90-95%. The average size of the nanoparticles was 205 nm, and the average zeta potential of them was -47 mV. In agar diffusion assay, an antibacterial activity (inhibition zone diameter, at 450 IU/mL) about 2 times higher than that of free nisin was observed for the nisin-loaded nanoparticles. MIC of the nisin-loaded nanoparticles (0.5 mg/mL) was about four times less than that of free nisin (2 mg/mL). Evaluation of the kinetic of the growth of S. aureus based on the total counts in the raw and pasteurized milks revealed that the nisin-loaded nanoparticles were able to inhibit more effectively the growth of S. aureus than free nisin during longer incubation periods. In other words, the decrease in the population of S. aureus for free nisin and the nisin-loaded nanoparticles in pasteurized milk was the same after 24 h of incubation while lessening in the growth of S. aureus was more marked for the nisin-loaded nanoparticles than the samples containing only free nisin after 48 h of incubation. Although the same growth reduction profile in S. aureus was noticed for free nisin and the nisin-loaded nanoparticles in the raw milk up to 14 h of incubation, after this time the nisin-loaded nanoparticles showed higher growth inhibition than free nisin. Since, generally, naked nisin has greater interactions with the ingredients present in milk samples in comparison with the protected nisin. Therefore, it is concluded that the antibacteri...

show abstract

Section: Microbial Assaysmentioning

confidence: 99%

Section: Standard Calibration Curve Of Nisinmentioning

confidence: 99%

Section: Standard Calibration Curve Of Nisinmentioning

confidence: 99%

Section: Activity Assay Of Nisin-loaded Nanoparticles With Agar Diffumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Comparative Study Between the Antibacterial Effect of Nisin and Nisin-Loaded Chitosan/Alginate Nanoparticles on the Growth of Staphylococcus aureus in Raw and Pasteurized Milk Samples

Zohri

Alavidjeh

Haririan

et al. 2010

Probiotics & Antimicro. Prot.

112

View full text Add to dashboard Cite

show abstract

Uso de nisina y quitosano para la inhibición de Staphylococcus aureus resistente a antibióticos y asociado a mastitis bovina

Sánchez-Ceja¹,

Arceo-Martínez²,

Sandoval-Flores³

et al. 2018

Rev. Mex. Cienc. Pecu.

View full text Add to dashboard Cite

En este trabajo se describen los patrones de resistencia a antibióticos de aislados de Staphylococcus aureus obtenidos de vacas con mastitis, así como su susceptibilidad in vitro a nisina, quitosano y al compósito nisina/quitosano. A partir de muestras de leche obtenidas de vacas enfermas se aislaron e identificaron 53 S. aureus, a los cuales se les analizó el perfil de resistencia a antibióticos. Los resultados evidenciaron una alta frecuencia de resistencia hacia el grupo de los beta-lactámicos, principalmente a penicilina, dicloxacilina y ampicilina. Asimismo, se detectó multirresistencia en algunos aislados, ya que mostraron tolerancia a 3 o 4 grupos de antibióticos, destacando los aislados AMC-9 (resistente a tres grupos) y AMC-23 (resistente a cuatro grupos). También se resalta que todos los aislados fueron susceptibles a levofloxacina. Por otra parte, se analizó la susceptibilidad in vitro de los aislados a nisina, quitosano y al compósito nisina/quitosano, mediante la determinación de la concentración mínima inhibitoria. Los resultados indican que el 100 % de los aislados fue susceptible a quitosano y al compósito nisina/quitosano y el 47.1 % a la nisina. El efecto del quitosano y del compósito nisina/quitosano sobre los aislados multirresistentes AMC-9 y AMC-23 fue bactericida. Estos resultados revelan el potencial de los compuestos antimicrobianos quitosano y nisina/quitosano para ser utilizados como agentes terapéuticos contra la mastitis bovina.

show abstract

Inhibitory efficacy of Lactococcus lactis on food born pathogens and food spoilage bacteria

Cited by 2 publications

References 3 publications

A Comparative Study Between the Antibacterial Effect of Nisin and Nisin-Loaded Chitosan/Alginate Nanoparticles on the Growth of Staphylococcus aureus in Raw and Pasteurized Milk Samples

A Comparative Study Between the Antibacterial Effect of Nisin and Nisin-Loaded Chitosan/Alginate Nanoparticles on the Growth of Staphylococcus aureus in Raw and Pasteurized Milk Samples

Uso de nisina y quitosano para la inhibición de Staphylococcus aureus resistente a antibióticos y asociado a mastitis bovina

Contact Info

Product

Resources

About