Inhibitory effects on the HMG-CoA Reductase in the chemical constituents of the <i>Cassia mimosoides</i> Linn

Shen, Chuangpeng; Huang, Liping; Xiang, Hua; Deng, Minzhen; Gao, Huahong; Zhu, Zhangzhi; Liu, Min; Luo, Gang

doi:10.1515/rrlm-2016-0041

Cited by 5 publications

(9 citation statements)

References 24 publications

(25 reference statements)

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“…Our sequential chromatographic experiments on the active fractions led to the isolation of 10 compounds, one of which was a mixture (Figure 1). Our structure elucidation studies indicated that five of them were abietane-type diterpenes (6,7-dehydroyleanone (1) [23], ferruginol (3) [24], horminone-7-acetoxyhorminone mixture (1:1) (6) (which were previously isolated from several Salvia species), 7-acetoxyhorminone (7) [25][26][27], and pisiferal (8) [28]); two nor abietane-type diterpenes (12-demethylmulticaulin (2) [29,30], and 12-hydroxy abieta-1, 3, 5 (10) 8, 11, 13-hexaene (4) [30,31]); one steroid (β-sitosterol (5) [32]); and two triterpenes (ursolic acid (9) [32] and oleanolic acid (10) [32]). Their structures were revealed using a combination of spectroscopic methods, e.g., UV, IR, 1 H-and 13 C-NMR-APT, HMQC, HMBC, and mass spectrometry (MS).…”

Section: Structure Elucidationmentioning

confidence: 89%

“…β-Sitosterol, as a white powder (8 mg) (5), and a mixture of horminone and 7-acetoxyhorminone (1:1) (15 mg, orange crystal) (6), were isolated from the M-6 fraction (petroleum ether-dichloromethane: 1/2 solvent system) with the help of preparative thin layer chromatography (TLC). 7-Acetoxyhorminone (7) was isolated from the M-7 fraction as an orange crystal (10 mg) using a Sephadex column, followed by preparative TLC (petroleum ether-dichloromethane: 1/3). Pisiferal (8) was isolated from the M-8…”

Section: Isolation Of the Compoundsmentioning

confidence: 99%

“…Pharmaceuticals 2022, 15,198 3 of 11 M-6 fraction (petroleum ether-dichloromethane: 1/2 solvent system) with the help of preparative thin layer chromatography (TLC). 7-Acetoxyhorminone (7) was isolated from the M-7 fraction as an orange crystal (10 mg) using a Sephadex column, followed by preparative TLC (petroleum ether-dichloromethane: 1/3). Pisiferal (8) was isolated from the M-8 fraction as a yellowish orange crystal, using a Sephadex column, followed by preparative TLC (dichloromethane-acetone: 1/1).…”

Section: Structure Elucidationmentioning

confidence: 99%

“…Figure 1. Chemical formulae of the isolated compounds: 6,7-dehydroyleanone (1), 12-demethylmulticaulin (2), ferruginol (3), 12-hydroxy abieta-1, 3, 5 (10), 8, 11, 13-hexaene (4), β-sitosterol (e5), horminone (6), 7-acetoxyhorminone (7), pisiferal (8), ursolic acid (9), and oleanolic acid (10). Chemical formulae of the isolated compounds: 6,7-dehydroyleanone (1), 12demethylmulticaulin (2), ferruginol (3), 12-hydroxy abieta-1, 3, 5 (10), 8, 11, 13-hexaene (4), β-sitosterol (e5), horminone (6), 7-acetoxyhorminone (7), pisiferal (8), ursolic acid (9), and oleanolic acid (10).…”

Section: Structure Elucidationmentioning

confidence: 99%

“…The HMG-CoA reductase inhibitory activity of the compounds isolated from the active fractions was also determined. Atorvastatin was used as the reference drug, while horminone-7-acetoxyhorminone mixture (1:1) (6) and 7-acetoxyhorminone (7) were found to possess the highest inhibition (Table 2).…”

Section: Hmg-coa Reductase Inhibitory Activity Of the Isolated Compoundsmentioning

confidence: 99%

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7-Acetoxyhorminone from Salvia multicaulis Vahl. as Promising Inhibitor of 3-Hydroxy-3-methylglutaryl Coenzyme A (HMG-CoA) Reductase

et al. 2022

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3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase is a key enzyme involved in cholesterol biosynthesis and one of the most important targets for the treatment of hypercholesterolemia. A limited number of studies on the HMG-CoA reductase inhibitory potential of natural products are available. Thus, in the current study, we aimed to test the HMG-CoA reductase inhibitory capacity of extracts from the roots and aerial parts of Salvia multicaulis Vahl., through activity-guided isolation. Our findings revealed that the root extract prepared with dichloromethane–acetone (1:1) showed the highest inhibition (71.97 ± 0.37%) at 100 µg/mL. The extract was then initially fractionated by column chromatography and the obtained fractions were monitored by thin layer chromatography. Fractions which were similar to each other were combined and a total of 15 fractions were obtained. Further conventional chromatographic studies were carried out on the active fractions. Based on these fractions, 10 known compounds, comprising 9 terpenes and 1 steroid derivative in total, were isolated and their structures were verified by a combination of IT-TOF-MS, and 1D and 2D NMR techniques. According to the enzyme inhibition data of the identified compounds, 7-acetoxyhorminone exerted the highest inhibition (84.15 ± 0.10%, IC50 = 63.6 ± 1.21 µg/mL). The molecular docking experiments on 7-acetoxyhorminone and horminone indicated that both compounds strongly bind to the active site of the enzyme.

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Section: Structure Elucidationmentioning

confidence: 89%

Section: Isolation Of the Compoundsmentioning

confidence: 99%

Section: Structure Elucidationmentioning

confidence: 99%

Section: Structure Elucidationmentioning

confidence: 99%

Section: Hmg-coa Reductase Inhibitory Activity Of the Isolated Compoundsmentioning

confidence: 99%

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7-Acetoxyhorminone from Salvia multicaulis Vahl. as Promising Inhibitor of 3-Hydroxy-3-methylglutaryl Coenzyme A (HMG-CoA) Reductase

et al. 2022

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Effects of different factors on seed germination of Cassia mimosoides

Liu

Liao

Xixian

et al. 2020

IOP Conf. Ser.: Earth Environ. Sci.

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In order to improve the germination efficiency of Cassia mimosoides seed, the effects of concentrated sulfuric acid, sodium hydroxide, temperature and culture medium on seed germination and seedling growth of C. mimosoides were investigated. In this study, the seeds were immersed in water at different temperatures and sodium hydroxide with different concentrations for 10 min, and the seeds were immersed in concentrated sulfuric acid for different periods of time, and then inoculated on media, the effects of the three treatment methods on germination of seeds were studied. It was found that concentrated sulfuric acid and sodium hydroxide had no effect on seed germination of C. mimosoides. The seed expansion rate, germination rate and other indexes were enhanced with the increase of temperature of soaking water, and when the water temperature was 80°C, the best result of seed germination was gained in C. mimosoides. Compared with MS medium, seed inoculation on 1/2MS medium was more beneficial to the growth and development of germinated seedlings.

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The Natural Products as Hydroxymethylglutaryl-Coa Reductase Inhibitors

Gülcan

Yigitkan

Orhan

2019

LDDD

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High cholesterol and triglyceride levels are mainly related to further generation of lifethreating metabolism disorders including cardiovascular system diseases. Therefore, hypercholesterolemia (i.e., also referred to as hyperlipoproteinemia) is a serious disease state, which must be controlled. Currently, the treatment of hypercholesterolemia is mainly achieved through the employment of statins in the clinic, although there are alternative drugs (e.g., ezetimibe, cholestyramine). In fact, the original statins are natural products directly obtained from fungi-like molds and mushrooms and they are potent inhibitors of hydroxymethylglutaryl-CoA reductase, the key enzyme in the biosynthesis of cholesterol. This review focuses on the first identification of natural statins, their synthetic and semi-synthetic analogues, and the validation of hydroxymethylglutaryl-CoA reductase as a target in the treatment of hypercholesterolemia. Furthermore, other natural products that have been shown to possess the potential to inhibit hydroxymethylglutaryl-CoA reductase are also reviewed with respect to their chemical structures.

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Inhibitory effects on the HMG-CoA Reductase in the chemical constituents of the Cassia mimosoides Linn

Cited by 5 publications

References 24 publications

7-Acetoxyhorminone from Salvia multicaulis Vahl. as Promising Inhibitor of 3-Hydroxy-3-methylglutaryl Coenzyme A (HMG-CoA) Reductase

7-Acetoxyhorminone from Salvia multicaulis Vahl. as Promising Inhibitor of 3-Hydroxy-3-methylglutaryl Coenzyme A (HMG-CoA) Reductase

Effects of different factors on seed germination of Cassia mimosoides

The Natural Products as Hydroxymethylglutaryl-Coa Reductase Inhibitors

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