Inhibitory Effects on Oral Microbial Activity and Production of Lipopolysaccharides-Induced Pro-Inflammatory Mediators in Raw264.7 Macrophages of Ethanol Extract of <i>Perilla flutescens</i> (L.) Britton

Jeong, Moon‐Jin; Lim, Do-Seon; Lee, Myoung-Hwa; Heo, Kyungwon; Kim, Han-Hong; Jeong, Soon‐Jeong

doi:10.17135/jdhs.2020.20.4.213

Cited by 3 publications

(8 citation statements)

References 21 publications

(33 reference statements)

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“…Stimulation by excessive oxidative stress in macrophages, monocytes, hepatocytes, bone marrow cells, and smooth muscle cells induces inflammation and immune responses and increases iNOS expression 18) . As a result, NO, an indicator of the inflammatory response, is produced 25) .…”

Section: Discussionmentioning

confidence: 99%

“…Total protein was isolated and quantified according to a previously described method 18) For the western blot analysis, the prepared total protein was electrophoresed on 10% sodium dodecyl sulfate polyacrylamide gels and transferred onto polyvinylidene difluoride membranes (Schleicher & Schuell, Keene, NH, USA). The membranes were probed with 1:1,000 anti-mouse inducible nitric oxide synthase (iNOS, BD Bioscience, San Jose, CA, USA) and 1:1,000 anti-mouse actin (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) primary antibodies.…”

Section: Protein Isolation and Western Blot Analysismentioning

confidence: 99%

“…NO is a representative pro-inflammatory mediator induced by lipopolysaccharide, H2O2, and ultraviolet irradiation, and its production is regulated by the synthesizing enzyme, iNOS 18) . The effects of CLE and NaHCO3 on NO generation and iNOS protein expression were confirmed in Raw264.7 cells (Fig.…”

Section: Inhibitory Effect Of Citrus Limon Extract Onmentioning

confidence: 99%

“…Recently, there has been increasing interest in revealing the functionality of natural products with fewer side effects and safety, and the use of natural products for the prevention and treatment of various diseases 18) . Citrus limon extract (CLE) is a citrus fruit that is consumed worldwide and contains various phenolic compounds and flavonoids 19) .…”

Section: Introductionmentioning

confidence: 99%

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Effects of Citrus limon Extract on Oxidative Stress-Induced Nitric Oxide Generation and Bovine Teeth Bleaching

Jeong

2021

J Dent Hyg Sci

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Background: We aimed to investigate the effect of Citrous limon extract (CLE) on oxidative stress-induced cytotoxicity and nitric oxide (NO) generation and the tooth bleaching effect of CLE as a substitute for hydrogen peroxide (H2O2) and determine the feasibility and application of CLE as a safe and effective natural tooth bleaching agent. Methods: The protective effect of CLE on H2O2-induced cytotoxicity in Raw264.7 macrophages was investigated by the MTT assay. The inhibitory effect of CLE on the generation of H2O2-induced NO was confirmed by the NO assay, and the changes in inducible nitric oxide synthase (iNOS) protein expression were confirmed by western blotting. Stained bovine teeth were treated with/without 15% and/or 35% CLE and H2O2, 15% sodium bicarbonate (NaHCO3) for 3 hours, and were irradiated with/without bleaching light (BL) for 15 minutes. The color change of the treated bovine tooth surface was measured using a colorimeter. Results: The viability of Raw264.7 cells treated with each concentration of CLE and 500 M H2O2 significantly increased as CLE increased, and NO generation and iNOS protein expression were significantly reduced in cells treated with 300 g CLE+/500 M H2O2+ and 300 g CLE+/500 M H2O2+/150 g NaHCO3+. The bleaching effect of 35% CLE+ was higher than that of 15% CLE+ and 15% NaHCO3+, and the effect was similar to that of 15% H2O2+. The 35% CLE+/15% NaHCO3+ showed the greatest bleaching effect and was higher than that of the groups irradiated with the BL. The greatest bleaching effect was observed with 35% CLE+/15% NaHCO3+, followed by 35% H2O2+/BL+. Conclusion: CLE inhibited oxidative stress-induced cytotoxicity and NO generation in Raw264.7 cells and, could replace H2O2, which causes side effects and risks in teeth breaching treatment. It showed greatest teeth bleaching effect when combined with NaHCO3. CLE is an effective and safe natural tooth bleaching substitute.

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Section: Discussionmentioning

confidence: 99%

Section: Protein Isolation and Western Blot Analysismentioning

confidence: 99%

Section: Inhibitory Effect Of Citrus Limon Extract Onmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Citrus limon Extract on Oxidative Stress-Induced Nitric Oxide Generation and Bovine Teeth Bleaching

Jeong

2021

J Dent Hyg Sci

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Print), 2231-5209 (Online) journals.ansfoundation.orgResearch Article ed by about 600 bacterial species, some of which are the cause of oral infectious diseases (Shaheena et al, 2019;Jeong et al, 2020). In order to maintain oral health and inhibit infectious oral diseases, including dental caries and periodontitis, control of oral microbial

…”

mentioning

confidence: 99%

Teeth bleaching effect and anti-oral microbial activity of water-extracted apple (Malus asiatica)

Jeong

2022

JANS

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Apple contains nutrients such as sugars, dietary fiber, and vitamins as well as bioactive phytochemicals such as organic acid, fatty acid and phenolic compounds. This study was conducted to confirm the applicability of apples as a bleaching agent and functional substances for the prevention of oral infectious diseases and maintenance of oral health by investigating the bovine teeth bleaching effect, anti-oral microbial activity, and inhibitory effect of halitosis of water-extracted apple (WEA). From the results of quantitative analysis of the surface color of bovine teeth, the application of WEA significantly increased the ΔE value, indicating a bleaching effect. In the case of 100mg/ml, the ΔE value increased as the WEA application time increased, and the bleaching effect was the greatest. Based on the results of the disk diffusion test and selective culture using CRT bacteria test kit, WEA showed anti-oral microbial activity against the dental caries bacteria, Streptococcus mutans and Latobacillus casei, the periodontal bacteria, Aggregatibacter actinomycetemcomitans, and Eschericha coli, but showed no antifungal effect against Candida albicans, causing oral candidiasis. From the results of colony formation and generation of halitosis from salivary microorganisms, WEA inhibited the growth of salivary microorganisms and generation of components inducing halitosis such as hydrogen sulfide, methyl mercaptan and dimethyl sulfide, that occur during the metabolic process of oral microorganisms. Therefore, WEA is a functional substance derived from a safe and useful natural product that can be used for the prevention of oral infectious diseases and maintenance of oral health

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Anti-Oral Microbial Effect of Ethanol Extract of Angelica gigas Nakai

Jeong

2024

J Dent Hyg Sci

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Background: The Korean name for Angelica gigas Nakai (AGN) is Cham-dang-gui, which grows naturally or is cultivated, and its dried roots are used in traditional herbal medicines. The AGN root exert various pharmacological effects. Despite the various pharmacological effects of the AGN root, there are no reports on its anti-oral microbial effects. The purpose of this study was to reveal the anti-oral microbial effect and the microbial and biochemical changes in oral microorganisms according to the concentration of the ethanol extract of AGN (EAGN) root, and to confirm the possibility of using EAGN as a plant-derived functional substance for controlling oral infectious microorganisms. Methods: Disk diffusion test, growth measurement, biofilm formation assay, and measurements of acid production and buffering capacity were performed to confirm the antibacterial effect of EAGN. Results: EAGN showed anti-oral bacterial effects against Streptococcus mutans and Aggregatibacter actinomycetemcomitans at all concentrations, with S. mutans showing a more susceptible effect at concentrations above 5.0 mg/ml and A. actinomycetemcomitans at 3.75 mg/ml. EAGN treatment significantly reduced A. actinomycetemcomitans growth at all concentrations tested. Biofilm formation was significantly reduced at concentrations above 3.75 mg/ml for S. mutans and 2.5 mg/ml for A. actinomycetemcomitans. Acid production in S. mutans and A. actinomycetemcomitans was significantly increased by treatment with EAGN, and the buffering capacities of S. mutans and A. actinomycetemcomitans increased from an EAGN concentration of 3.75 mg/ml and above. Conclusion: EAGN showed anti-oral bacterial effects against both S. mutans and A. actinomycetemcomitans at concentrations above 3.75 mg/ml, which were thought to be related to the inhibition of their growth and biofilm formation. Therefore, EAGN can be used as a safe functional substance derived from medicinal plants owing to its antibacterial effects against S. mutans and A. actinomycetemcomitans.

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Inhibitory Effects on Oral Microbial Activity and Production of Lipopolysaccharides-Induced Pro-Inflammatory Mediators in Raw264.7 Macrophages of Ethanol Extract of Perilla flutescens (L.) Britton

Cited by 3 publications

References 21 publications

Effects of Citrus limon Extract on Oxidative Stress-Induced Nitric Oxide Generation and Bovine Teeth Bleaching

Effects of Citrus limon Extract on Oxidative Stress-Induced Nitric Oxide Generation and Bovine Teeth Bleaching

Teeth bleaching effect and anti-oral microbial activity of water-extracted apple (Malus asiatica)

Anti-Oral Microbial Effect of Ethanol Extract of Angelica gigas Nakai

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