Inhibitory effects of wild dietary plants on lipid peroxidation and on the proliferation of human cancer cells

Marrelli, Mariangela; Cristaldi, Brigida; Menichini, Francesco; Conforti, Filomena

doi:10.1016/j.fct.2015.09.011

Cited by 51 publications

(22 citation statements)

References 25 publications

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“…20 The action of different types of R. officinalis L. extracts was reported on some tumor cell lines such as the ovarian cancer (SK-OV-3 and HO-8910), hepatocellular carcinoma (Bel-7402), 21 colorectal cancer (SW620 and DLD-1), pancreas cancer (MIA-PaCa-2 and PANC-1), 22 prostate cancer (LNCaP and 22Rv1), 23 colorectal adenocarcinoma (LoVo), hepatocarcinoma (HepG2). On the target lineages in this study, MCF-7 24 and HeLa, 25 the action of R. officinalis L has also been reported; however, in our study, we used three different cell viability tests and glycolic extract.…”

Section: Introductionmentioning

confidence: 99%

Biological activities of Rosmarinus officinalis L. (rosemary) extract as analyzed in microorganisms and cells

Oliveira

Jesus

Figueira

et al. 2017

Exp Biol Med (Maywood)

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Rosmarinus officinalis L. extract effectively contributed to in vitro control of important species of microorganisms such as Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans, and Pseudomonas aeruginosa in mono-and polymicrobial biofilms that are responsible for several infections in oral cavity as in other regions of the body. Furthermore, this extract promoted also cell viability above 50% at concentrations 50 mg/mL, excellent anti-inflammatory effect, showing inhibition or reduction of the synthesis of proinflammatory cytokines, being also non-genotoxic to cell lines studied. Thus, this extract may be a promising therapeutic agent that can be added in some medical and dental formulations such as toothpastes, mouthwashes, irrigating root canals, ointments, soaps, in order to control pathogenic microorganisms and biofilms, with anti-inflammatory effect and absence of cytotoxic and genotoxic. AbstractR. officinalis L. is an aromatic plant commonly used as condiment and for medicinal purposes. Biological activities of its extract were evaluated in this study, as antimicrobial effect on mono-and polymicrobial biofilms, cytotoxicity, anti-inflammatory capacity, and genotoxicity. Monomicrobial biofilms of Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa and polymicrobial biofilms composed of C. albicans with each bacterium were formed in microplates during 48 h and exposed for 5 min to R. officinalis L. extract (200 mg/mL). Its cytotoxic effect was examined on murine macrophages (RAW 264.7), human gingival fibroblasts (FMM-1), human breast carcinoma cells (MCF-7), and cervical carcinoma cells (HeLa) after exposure to different concentrations of the extract, analyzed by MTT, neutral red (NR), and crystal violet (CV) assays. The anti-inflammatory activity was evaluated on RAW 264.7 non-stimulated or stimulated by lipopolysaccharide (LPS) from Escherichia coli and treated with different concentrations of the extract for 24 h. Interleukin-1 beta (IL-1b) and tumor necrosis factor alpha (TNF-a) were quantified by ELISA. Genotoxicity was verified by the frequency of micronuclei (MN) at 1000 cells after exposure to concentrations of the extract for 24 h. Data were analyzed by T-Test or ANOVA and Tukey Test (P 0.05). Thus, significant reductions in colony forming units per milliliter (CFU/mL) were observed in all biofilms. Regarding the cells, it was observed that concentrations 50 mg/mL provided cell viability of above 50%. Production of proinflammatory cytokines in the treated groups was similar or lower compared to the control group. The MN frequency in the groups exposed to extract was similar or less than the untreated group. It was shown that R. officinalis L. extract was effective on mono-and polymicrobial biofilms; it also provided cell viability of above 50% (at 50 mg/mL), showed anti-inflammatory effect, and was not genotoxic.

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Section: Introductionmentioning

confidence: 99%

Biological activities of Rosmarinus officinalis L. (rosemary) extract as analyzed in microorganisms and cells

Oliveira

Jesus

Figueira

et al. 2017

Exp Biol Med (Maywood)

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“…26 In brief, 1 mL of EtOH was added to 2 mg of crude extract. After 5 min of incubation, 1 mL di 2% AlCl 3 aqueos solution was added and the mixture was allowed to stand for 15 min.…”

Section: Determination Of Total Flavonoidsmentioning

confidence: 99%

Antioxidant, Enzyme-Inhibitory and Antitumor Activity of the Wild Dietary Plant Muscari comosum (L.) Mill.

Casacchia

Sofo

Casaburi

et al. 2017

IJPB

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Conventional medicines used to treat obesity and cancer frequently exhibit high side effects, so that researchers are focusing on new therapies and drugs based on natural products. Total extracts from bulbs of Muscari comosum were tested for i) free radical scavenging activity, ii) in vitro enzymatic inhibition of pancreatic α-amylase and lipase, and iii) inhibition of the growth of breast adenocarcinoma cells. Three treatments were considered: bulbs boiled in water for 15 min (traditional cooking method; BB); bulbs steam-cooked for 15 min (alternative cooking method; SB); raw bulbs (RB). The polyphenol content and antioxidant capacity of bulb extracts were related to the inhibition of pancreatic lipase and α-amylase, whose activities have been found to have a half maximal inhibitory concentration (IC 50 ) of 0.28, 2.14 and 3.22 mg/mL for lipase, and 0.16, 0.73 and 0.69 mg/mL for α-amylase in RB, SB and BB, respectively. The analysis on breast adenocarcinoma MCF-7 cells revealed that RB extracts, and in a lesser extent BB, exerted a dose-dependent inhibition on cell proliferation. Considering that the potential of natural products for the treatment of obesity are under exploration, M. comosum could be an excellent plant for the development of future anti-obesity drugs, also able to prevent cancer.

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“…The flavonoid content was determined spectrophotometrically using a method based on the formation of a flavonoidaluminum complex [9]. Absorbance was measured at 510 nm.…”

Section: Determination Of Total Phenolic and Flavonoid Contentmentioning

confidence: 99%

“…Nitrite concentration in the supernatants was determined by comparison with a sodium nitrite standard curve. The absence of cytotoxic effects was assessed by means of the MTT [(3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide)] test, as previously described [9].…”

Section: Inhibition Of Nitric Oxide (No) Production In Lps-stimulatedmentioning

confidence: 99%

Hypolipidemic and Antioxidant Properties of Hot Pepper Flower (Capsicum annuum L.)

Marrelli

Menichini

Conforti

2016

Plant Foods Hum Nutr

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At present, the various medical treatments of obesity involve side effects. The aim of the research is therefore to find natural compounds that have anti-obesity activity with minimum disadvantages. In this study, the hypolipidemic effect of hydroalcoholic extract of flowers from Capsicum annuum L. was examined through the evaluation of inhibition of pancreatic lipase. Antioxidant activity was assessed using different tests: 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (˙NO) and lipid peroxidation inhibition assays. Phytochemical analysis indicated that total phenolic and flavonoid content in the extract was 128.7 ± 4.5 mg chlorogenic acid equivalent/g of crude extract and 17.66 ± 0.11 mg of quercetin equivalent/g of crude extract, respectively. The extract inhibited pancreatic lipase with IC50 value equal to 3.54 ± 0.18 mg/ml. It also inhibited lipid peroxidation with IC50 value of 27.61 ± 2.25 μg/ml after 30 min of incubation and 41.69 ± 1.13 μg/ml after 60 min of incubation. The IC50 value of radical scavenging activity was 51.90 ± 2.03 μg/ml. The extract was also able to inhibit NO production (IC50 = of 264.3 ± 7.98 μg/ml) without showing any cytotoxic effect.

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Inhibitory effects of wild dietary plants on lipid peroxidation and on the proliferation of human cancer cells

Cited by 51 publications

References 25 publications

Biological activities of Rosmarinus officinalis L. (rosemary) extract as analyzed in microorganisms and cells

Biological activities of Rosmarinus officinalis L. (rosemary) extract as analyzed in microorganisms and cells

Antioxidant, Enzyme-Inhibitory and Antitumor Activity of the Wild Dietary Plant Muscari comosum (L.) Mill.

Hypolipidemic and Antioxidant Properties of Hot Pepper Flower (Capsicum annuum L.)

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