Inhibitory effects of tricin derivative from Sasa albo-marginata on replication of human cytomegalovirus

Akuzawa, Kazuhiko; Yamada, Rie; Li, Zhuan; Liu, Ying; Sadanari, Hidetaka; Matsubara, Keiko; Watanabe, Kunitomo; Koketsu, Mamoru; Tuchida, Yuuzo; Murayama, Tsugiya

doi:10.1016/j.antiviral.2011.06.014

Cited by 23 publications

(31 citation statements)

References 44 publications

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“…Tricin dose-dependently inhibited CXCL11 gene expression at concentrations ranging from 0.1 mM to 10 mM, leading to up-regulated CXCL11 gene expression after HCMV infection. Studies on action mechanisms are consistent with tricin mainly affecting the immediate early events of viral replication [24]. We demonstrated similar effects of tricin that induced decreased expression of CXCL11 mRNA until 9 and 24 h after HCMV infection of immediate early and/or early stages, and expression increased at 48 h after infection of late stages ( Fig.…”

Section: Discussionsupporting

confidence: 80%

“…The inhibition of IE gene expression and subsequent chemokine secretion that we observed after treatment with tricin may thus be advantageous for GCV or PFA, as these drugs cannot prevent virus-mediated immunopathogenesis. Similarly, in a recent report, tricin was found to dose-dependently reduce the protein expression of viral IE and cyclooxigenese-2 (COX-2) in an in vitro MRC-5 cell cultured model [24].…”

Section: Discussionmentioning

confidence: 92%

“…The importance of IE function and the inability of currently available antiviral therapy to prevent their expression suggests that pharmacological manipulation of their expression and/or function provides an alternative strategy to prevent HCMV reactivation, replication and immunopathogenesis [24,37,38]. It is necessary, therefore, to identify novel anti-cytomegaloviral agents that can block HCMV gene expression in very early stages without causing major adverse side effects.…”

Section: Discussionmentioning

confidence: 98%

“…1), and were suspended in dimethyl sulfoxide (DMSO) as described previously [24]. Briefly, we prepared tricin through a condensation reaction of methyl benzoate 1 with acetophenone 2, followed by acid cyclodehydration.…”

Section: Compoundmentioning

confidence: 99%

“…1), has anti-HCMV activity in a human embryonic fibroblast cell line [23,24]. As the development of drug resistance is a constant concern, the search for new antiviral agents from a variety of sources, including plants, has become more urgent [25].…”

Section: Introductionmentioning

confidence: 99%

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Anti-cytomegalovirus effects of tricin are dependent on CXCL11

Murayama

Liu

Takahashi

et al. 2012

Microbes and Infection

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 98%

Section: Compoundmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti-cytomegalovirus effects of tricin are dependent on CXCL11

Murayama

Liu

Takahashi

et al. 2012

Microbes and Infection

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The anti‐human cytomegalovirus drug tricin inhibits cyclin‐dependent kinase 9

et al. 2018

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4′,5,7‐trihydroxy‐3′,5′‐dimethoxyflavone (tricin), derived from Sasa albo‐marginata, has been reported to suppress significantly human cytomegalovirus (HCMV) replication in human embryonic lung (HEL) fibroblast cells. However, the target protein of tricin remains unclear. This study focused on the anti‐HCMV activity of tricin in terms of its binding affinity to cyclin‐dependent kinase 9 (CDK9). A molecular docking study predicted that tricin binds well to the ATP‐binding site of CDK9. Experimental measurements then revealed that tricin inhibits the kinase activity of CDK9 and affects the phosphorylation of the carboxy‐terminal domain of RNA polymerase II. Based on these results, we conclude that CDK9 is one of the target proteins of tricin. We also found that tricin possesses anti‐HCMV activity with no cytotoxicity against HEL cells.

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Chemical defense responses of upland cotton, Gossypium hirsutum L. to physical wounding

Park

Scheffler

et al. 2019

Plant Direct

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Upland cotton ( Gossypium hirsutum L.) produces terpenoid aldehydes ( TA s) that protect the plant from microbial and insect infestations. Foliar TA s include plus (+)‐ and minus (−)‐gossypol, hemigossypolone, and heliocides. To examine foliar TA s’ response to physical wounding, the four TA derivatives of a fully glanded G. hirsutum variety JACO GL were quantified by ultra‐high performance liquid chromatography. The results show that foliar heliocides increased by 1.7‐fold in younger leaves after wounding. While the hemigossypolone level was not affected by the physical wounding, the level of heliocides was significantly increased up to 1.8‐fold in the younger leaves. Upland cotton accumulates concentrated carbohydrates, amino acids, and fatty acids in foliar extrafloral nectar ( EFN ) to serve as a nutrient resource, which attracts both beneficial insects and damaging pests. To better understand the nectar physiology, particularly to determine the temporal dynamics of EFN metabolites in response to the wounding, a gas chromatograph‐mass spectrometer ( GC ‐ MS ) was used to perform metabolic profiling analyses of a G. hirsutum variety Deltapine 383 that has fully developed extrafloral nectaries. A total of 301 compounds were monitored, specifically 75 primary metabolites, two secondary metabolites and 224 unidentified compounds. The physical wounding treatment changed the EFN composition and lowered overall production. The accumulation of 30 metabolites was altered in response to the wounding treatment and threonic acid levels increased consistently. GC ‐ MS combined with Kovat's analysis enabled identification of EFN secondary metabolites including furfuryl alcohol and 5‐hyrdomethoxyfurfural, which both have antioxidant and antimicrobial properties that may protect the nectar against microbial pathogens. This study provides new insights into the wounding response of cotton plants in terms of cotton metabolites found in leaf glands and extrafloral nectar as well as highlighting some protective functions of secondary metabolites produced in foliar glands and extrafloral nectaries.

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Inhibitory effects of tricin derivative from Sasa albo-marginata on replication of human cytomegalovirus

Cited by 23 publications

References 44 publications

Anti-cytomegalovirus effects of tricin are dependent on CXCL11

Anti-cytomegalovirus effects of tricin are dependent on CXCL11

The anti‐human cytomegalovirus drug tricin inhibits cyclin‐dependent kinase 9

Chemical defense responses of upland cotton, Gossypium hirsutum L. to physical wounding

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