Inhibitory effects of the essential oils α-longipinene and linalool on biofilm formation and hyphal growth of <i>Candida albicans</i>

Manoharan, Ranjith Kumar; Lee, Jin-Hyung; Kim, Yong‐Guy; Kim, Soon-Il; Lee, Jintae

doi:10.1080/08927014.2017.1280731

Cited by 62 publications

(40 citation statements)

References 56 publications

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“…Initially, we investigate whether indole derivatives affect biofilm formation by fluconazole‐resistant C. albicans DAY185 (Manoharan et al ., 2017a,b), and cell growth was simultaneously measured in the presence of indole derivatives. Of the 34 commercially available indole derivatives examined, 7‐benzyloxyindole, 4‐fluoroindole and 5‐iodoindole significantly reduced biofilm formation at concentrations of 0.1 and 0.5 mM (Table S1).…”

Section: Resultsmentioning

confidence: 99%

“…As previously reported, DAY185 is resistant to the commercial antifungal fluconazole (MIC ~ 512 μg ml −1 ) (Manoharan et al ., 2017a,b). A single colony was inoculated into 25 ml of PDB and incubated for overnight at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Caenorhabditis elegans strain N2 Bristol CF512 fer‐15 (b26) ; fem‐1 (hc17) (Manoharan et al ., 2017a,b) was used to perform C. albicans virulence assay using the protocol described by (Manoharan et al ., 2017a,b). Briefly, synchronized adults worms were fed on C. albicans lawns for 4 h at 25°C and collected after washing three times with M9 buffer.…”

Section: Methodsmentioning

confidence: 99%

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Efficacy of 7‐benzyloxyindole and other halogenated indoles to inhibit Candida albicans biofilm and hyphal formation

Manoharan

Lee

2018

Microbial Biotechnology

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SummaryCertain pathogenic bacteria and yeast form biofilms on biotic and abiotic surfaces including medical devices and implants. Hence, the development of antibiofilm coating materials becomes relevant. The virulence of those colonizing pathogens can be reduced by inhibiting biofilm formation rather than killing pathogens using excessive amounts of antimicrobials, which is touted as one of the main reasons for the development of drug resistance. Candida albicans is an opportunistic fungal pathogen, and the transition of yeast cells to hyphal cells is believed to be a crucial virulence factor. Previous studies have shown that indole and its derivatives possess antivirulence properties against various bacterial pathogens. In this study, we used various indole derivatives to investigate biofilm‐inhibiting activity against C. albicans. Our study revealed that 7‐benzyloxyindole, 4‐fluoroindole and 5‐iodoindole effectively inhibited biofilm formation compared to the antifungal agent fluconazole. Particularly, 7‐benzyloxyindole at 0.02 mM (4.5 μg ml−1) significantly reduced C. albicans biofilm formation, but had no effect on planktonic cells, and this finding was confirmed by a 2,3‐bis‐(2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐2H‐tetrazolium‐5‐carboxanilide (XTT) assay and three‐dimensional confocal laser scanning microscopy. Scanning electron microscopy analyses revealed that 7‐benzyloxyindole effectively inhibited hyphal formation, which explains biofilm inhibition. Transcriptomic analysis showed that 7‐benzyloxyindole downregulated the expressions of several hypha/biofilm‐related genes (ALS3,ECE1,HWP1 and RBT1). A C. albicans‐infected Caenorhabditis elegans model system was used to confirm the antivirulence efficacy of 7‐benzyloxyindole.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Efficacy of 7‐benzyloxyindole and other halogenated indoles to inhibit Candida albicans biofilm and hyphal formation

Manoharan

Lee

2018

Microbial Biotechnology

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“…For instance, essential oils like citronella and cinnamon have been reported to have antibiofilm and anticandida activities (Almeida Lde et al, 2016 ). Recently, we found cascarilla bark and helichyrsum oil strongly inhibited Candida biofilm and hyphal formation (Manoharan et al, 2017 ). There are no data on antibiofilm activity of cedar leaf essential oil (CLEO) derived from the Cedar of Lebanon ( Cedrus libani ), which is found in Lebanon and south central Turkey.…”

Section: Introductionmentioning

confidence: 92%

Antibiofilm and Antihyphal Activities of Cedar Leaf Essential Oil, Camphor, and Fenchone Derivatives against Candida albicans

Manoharan

Lee

2017

Front. Microbiol.

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Candida albicans can form biofilms composed of yeast, hyphal, and pseudohyphal elements, and C. albicans cells in the hyphal stage could be a virulence factor. The present study describes the chemical composition, antibiofilm, and antihyphal activities of cedar leaf essential oil (CLEO), which was found to possess remarkable antibiofilm activity against C. albicans but not to affect its planktonic cell growth. Nineteen components were identified in CLEO by gas chromatography/mass spectrometry, and phenolics were the main constituents. Of these, camphor, fenchone, fenchyl alcohol, α-thujone, and borneol significantly reduced C. albicans biofilm formation. Notably, treatments with CLEO, camphor, or fenchyl alcohol at 0.01% clearly inhibited hyphal formation, and this inhibition appeared to be largely responsible for their antibiofilm effects. Transcriptomic analyses indicated that camphor and fenchyl alcohol downregulated some hypha-specific and biofilm related genes (ECE1, ECE2, RBT1, and EED1). Furthermore, camphor and fenchyl alcohol reduced C. albicans virulence in a Caenorhabditis elegans nematode model. These results demonstrate CLEO, camphor, and fenchyl alcohol might be useful for controlling C. albicans infections.

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“…Recently, several EOs and phytochemicals have been reported to inhibit biofilm formation by bacteria and fungi [15,16], and their effects on P. aeruginosa have been studied [17,18]. Taking into consideration the same plant variety, EO composition can differ according to geographical region, seasonality, and extraction methodology [19,20,21].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Activity and Machine Learning Classification Analysis of Essential Oils from Different Mediterranean Plants against Pseudomonas aeruginosa

et al. 2018

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Pseudomonas aeruginosa is a ubiquitous organism and opportunistic pathogen that can cause persistent infections due to its peculiar antibiotic resistance mechanisms and to its ability to adhere and form biofilm. The interest in the development of new approaches for the prevention and treatment of biofilm formation has recently increased. The aim of this study was to seek new non-biocidal agents able to inhibit biofilm formation, in order to counteract virulence rather than bacterial growth and avoid the selection of escape mutants. Herein, different essential oils extracted from Mediterranean plants were analyzed for their activity against P. aeruginosa. Results show that they were able to destabilize biofilm at very low concentration without impairing bacterial viability. Since the action is not related to a bacteriostatic/bactericidal activity on P. aeruginosa, the biofilm change of growth in presence of the essential oils was possibly due to a modulation of the phenotype. To this aim, application of machine learning algorithms led to the development of quantitative activity–composition relationships classification models that allowed to direct point out those essential oil chemical components more involved in the inhibition of biofilm production. The action of selected essential oils on sessile phenotype make them particularly interesting for possible applications such as prevention of bacterial contamination in the community and in healthcare environments in order to prevent human infections. We assayed 89 samples of different essential oils as P. aeruginosa anti-biofilm. Many samples inhibited P. aeruginosa biofilm at concentrations as low as 48.8 µg/mL. Classification of the models was developed through machine learning algorithms.

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Inhibitory effects of the essential oils α-longipinene and linalool on biofilm formation and hyphal growth of Candida albicans

Cited by 62 publications

References 56 publications

Efficacy of 7‐benzyloxyindole and other halogenated indoles to inhibit Candida albicans biofilm and hyphal formation

Efficacy of 7‐benzyloxyindole and other halogenated indoles to inhibit Candida albicans biofilm and hyphal formation

Antibiofilm and Antihyphal Activities of Cedar Leaf Essential Oil, Camphor, and Fenchone Derivatives against Candida albicans

Antimicrobial and Antibiofilm Activity and Machine Learning Classification Analysis of Essential Oils from Different Mediterranean Plants against Pseudomonas aeruginosa

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