Inhibitory effects of the dietary flavonoid quercetin on the enzyme activity of zinc(II)-dependent yeast alcohol dehydrogenase: Spectroscopic and molecular docking studies

Bhuiya, Sutanwi; Haque, Lucy; Pradhan, Ankur Bikash; Das, Suman

doi:10.1016/j.ijbiomac.2016.11.047

Cited by 24 publications

(21 citation statements)

References 46 publications

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“…Naturally occurring small organic molecules have always attracted research interest because of their varied biological attributes (Bhuiya et al, 2017 ). At the same time, proteins perform numerous important roles in organismal biology, including in metabolic activity, catalysis, immune responses, and signal transduction.…”

Section: Introductionmentioning

confidence: 99%

“…Many of these processes involve interactions between proteins and small organic molecules, and lead to subsequent changes in the conformation of the former. As a result, these modified proteins can induce positive or negative effects in organisms, while the organic molecules can function by acting as signaling substances, auxin analogs, and active components (Antunes et al, 2011 ; E et al, 2015 ; Bhuiya et al, 2017 ). A number of methods to study these interactions have therefore been developed in recent years, including the bioinformatics tools molecular docking (Skrt et al, 2012 ; Bhuiya et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

“…As a result, these modified proteins can induce positive or negative effects in organisms, while the organic molecules can function by acting as signaling substances, auxin analogs, and active components (Antunes et al, 2011 ; E et al, 2015 ; Bhuiya et al, 2017 ). A number of methods to study these interactions have therefore been developed in recent years, including the bioinformatics tools molecular docking (Skrt et al, 2012 ; Bhuiya et al, 2017 ). This tool enables an understanding of the three dimensional (3D) structure of receptor proteins and organic molecular ligands via continuous optimization of the location, conformation, and rotated dihedral angle within molecules, as well as amino acid side chains and skeleton of the receptor.…”

Section: Introductionmentioning

confidence: 99%

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Organic Molecules from Biochar Leacheates Have a Positive Effect on Rice Seedling Cold Tolerance

et al. 2017

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Biochar is known to have a number of positive effects on plant ecophysiology. However, limited research has been carried out to date on the effects and mechanisms of biochar on plant ecophysiology under abiotic stresses, especially responses to cold. In this study, we report on a series of experiments on rice seedlings treated with different concentrations of biochar leacheates (between 0 and 10% by weight) under cold stress (10°C). Quantitative real-time PCR (qRT-PCR) and cold-resistant physiological indicator analysis at low temperatures revealed that the cold tolerance of rice seedlings increased after treatment with high concentrations of biochar leacheates (between 3 and 10% by weight). Results also show that the organic molecules in biochar leacheates enhance the cold resistance of plants when other interference factors are excluded. We suggest that the positive influence of biochar on plant cold tolerance is because of surface organic molecules which likely function by entering a plant and interacting with stress-related proteins. Thus, to verify these mechanisms, this study used gas chromatography-mass spectrometry (GC-MS) techniques, identifying 20 organic molecules in biochar extracts using the National Institute of Standards and Technology (NIST) library. Further, to illustrate how these organic molecules work, we utilized the molecular docking software Autodock to show that the organic molecule 6-(Methylthio)hexa-1,5-dien-3-ol from biochar extracts can dock with the stress-related protein zinc-dependent activator protein (ZAP1). 6-(Methylthio)hexa-1,5-dien-3-ol has a similar binding mode with the ligand succinic acid of ZAP1. It can be inferred that the organic molecule identified in this study performs the same function as the ZAP1 ligand, stimulating ZAP1 driving cold-resistant functions, and enhancing plant cold tolerance. We conclude that biochar treatment enhances cold tolerance in rice seedlings via interactions between organic molecules and stress related proteins.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Organic Molecules from Biochar Leacheates Have a Positive Effect on Rice Seedling Cold Tolerance

et al. 2017

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“…And then, the ADH acts to reduce the aldehydes to their corresponding alcohols. Finally, the alcohols are esterified at the present of the alcohol acetyltransferase (Bhuiya, Haque, Pradhan, & Das, 2017;Cai, 1997;Oey, Lille, Van Loey, & Hendrickx, 2008;Zhang et al, 2014). In this pathway, the increase in the LOX activity or/and the decrease in the ADH activity would benefit the accumulation of the C9 aldehyde compounds.…”

Section: Effect Of Uht On Formation Of the Aroma Of The Watermelon mentioning

confidence: 99%

Effect of ultrahigh temperature treatment on qualities of watermelon juice

Wang

Guo

et al. 2018

Food Science & Nutrition

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The watermelon juice was pasteurized by the ultrahigh temperature (UHT) treatment, and the qualities of the pasteurized juice were compared to screen the optimal UHT. The UHT of 120 and 135°C inactivated the microbial colonies and maintained the original color of the watermelon juice. The temperature of 120 and 135°C was also maintained the phenolic content by reducing the polyphenoloxidase activity. Moreover, the C9 aldehydes, especially the (E,Z)‐2,6‐nonadienal, presented the main aroma of the watermelon juice. The C9 aldehydes were formed as the results of the heat reduction and enzymatic metabolism. The temperature of 120 and 135°C reduced the alcohol dehydrogenase activity and well maintained the C9 aldehyde content of the watermelon juice. Hence, the temperature of 120°C of the UHT treatment was the optimal temperature for the production of the watermelon juice.

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“…5 Quercetin (3,5,7,3'-4'-pentahydroxy-flavone) is one of the most abundant flavonoids, classified as a flavonol, with antioxidant and antitumoral activity due to the ability to act as a free radical scavenger. 6 The action of quercetin in the process of inhibiting the formation of free radicals can occur in three different stages, at initiation (by interaction with superoxide ions), in the formation of hydroxyl radicals (by chelating iron ions) and in lipid peroxidation (by reacting with peroxyl radicals of lipids). 7 In addition, quercetin also has antiallergic, antiviral, antimutagenic, cardiovascular protection, cataract prevention, and lipid peroxidation inhibitory properties.…”

Section: Introductionmentioning

confidence: 99%

Use of Carbon Dots Synthesized from Citrate as a Fluorescent Probe for Quercetin Determination in Tea and Beer Samples

Paula¹,

Milani²,

Lavorante³

et al. 2019

J. Braz. Chem. Soc.

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In this work, carbon dots (CDs) prepared by a one-step hydrothermal method were employed for the determination of quercetin in teas and beers. The fluorescence quenching of CDs was proportional to the concentration of quercetin. Under optimum experimental conditions, linear quenching was observed for quercetin in the range of 1.0 to 10 mg L-1 (F/F 0 = (0.1511 ± 0.0075)[Q] + (0.8922 ± 0.0384), R 2 = 0.9926), at room temperature, using 80 μL CDs and 0.1 mol L-1 Na 2 HPO 4 /NaOH buffer solution (pH 11.0). A limit of detection (3σ criterion) of 0.85 mg L-1 , and a relative standard deviation of 0.21% (n = 3) for 4.0 mg L-1 quercetin solution was obtained. Addition and recovery tests with tea and beer samples were performed resulting in recoveries at the range between 80 and 118%. This procedure served successfully to determine quercetin in beer and tea samples presenting high sample throughput.

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Inhibitory effects of the dietary flavonoid quercetin on the enzyme activity of zinc(II)-dependent yeast alcohol dehydrogenase: Spectroscopic and molecular docking studies

Cited by 24 publications

References 46 publications

Organic Molecules from Biochar Leacheates Have a Positive Effect on Rice Seedling Cold Tolerance

Organic Molecules from Biochar Leacheates Have a Positive Effect on Rice Seedling Cold Tolerance

Effect of ultrahigh temperature treatment on qualities of watermelon juice

Use of Carbon Dots Synthesized from Citrate as a Fluorescent Probe for Quercetin Determination in Tea and Beer Samples

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