Inhibitory Effects of Synthetic Peptides Containing Bovine Lactoferrin C-lobe Sequence on Bacterial Growth

Kim, Woan-Sub; Ohashi, Midori; Shimazaki, K.

doi:10.5851/kosfa.2016.36.4.452

Cited by 6 publications

(3 citation statements)

References 37 publications

(43 reference statements)

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“…and E . coli strains that reduce milk quality [98]. Based on these results, some hypothetical mechanisms that may account for the effects of Lf on in vitro probiotic growth are depicted in Figure 2.…”

Section: Lactoferrin: Effects On Probiotic Growthmentioning

confidence: 99%

The Impact of Lactoferrin on the Growth of Intestinal Inhabitant Bacteria

Vega-Bautista

Garza

Carrero

et al. 2019

IJMS

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Lactoferrin (Lf) is an iron-binding milk glycoprotein that promotes the growth of selected probiotic strains. The effect of Lf on the growth and diversification of intestinal microbiota may have an impact on several issues, including (i) strengthening the permeability of the epithelial cell monolayer, (ii) favoring the microbial antagonism that discourages the colonization and proliferation of enteric pathogens, (iii) enhancing the growth and maturation of cell-monolayer components and gut nerve fibers, and (iv) providing signals to balance the anti- and pro-inflammatory responses resulting in gut homeostasis. Given the beneficial role of probiotics, this contribution aims to review the current properties of bovine and human Lf and their derivatives in in vitro probiotic growth and Lf interplay with microbiota described in the piglet model. By using Lf as a component in pharmacological products, we may enable novel strategies that promote probiotic growth while conferring antimicrobial activity against multidrug-resistant microorganisms that cause life-threatening diseases, especially in neonates.

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Section: Lactoferrin: Effects On Probiotic Growthmentioning

confidence: 99%

The Impact of Lactoferrin on the Growth of Intestinal Inhabitant Bacteria

Vega-Bautista

Garza

Carrero

et al. 2019

IJMS

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“…The reason for this may be that as a Grampositive bacterium, S. aureus has a thicker cell wall than the Gram-negative bacteria, E. coli JM109 and P. aeruginosa. Lactoferrin is widely considered to be an important component of the host defense mechanism against microbial infection [25]. Recent research indicated that the bactericidal effect of BLF is directly related to the interaction between BLF and bacteria [26,27].…”

Section: Discussionmentioning

confidence: 99%

Heterologous expression of bovine lactoferrin C-lobe in Bacillus subtilis and comparison of its antibacterial activity with N-lobe

Jin

Zhang

et al. 2021

Syst Microbiol and Biomanuf

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The natural concentration of bovine lactoferrin C-lobe is low and its separation by proteolytic enzyme digestion is difficult. Here, we expressed the codon-optimized fragment of C-lobe on plasmid pMA0911 with the P veg promoter in Bacillus subtilis 168 at 20 °C. The yield was 7.5 mg/L, and 90.6% purity was achieved using ammonium sulfate precipitation, Ni-NTA and molecular exclusion. The C-lobe at 10 mg/mL completely inhibited cell growth of Escherichia coli JM109 (DE3) and Pseudomonas aeruginosa CGMCC 1.6740, and 48.4% of growth of Staphylococcus aureus CGMCC 1.282, the result is similar to that of 200 ng/mL N-lobe. The minimum inhibitory concentrations of C-lobe were 4, 8 and 16 mg/mL, while those of N-lobe were 128, 256 and 512 μg/mL for E. coli, P. aeruginosa and S. aureus, respectively. This is the first report on bovine lactoferrin C-lobe expression and the comparative resistance of the recombinant N-and C-lobes in a food-safe strain of B. subtilis. Our findings offer the potential to study the structure-function relationship of the N-and C-lobes recombinantly produced in the same host.

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“…Furthermore, compared with the N-lobe, the C-lobe of transferrin has shown stronger growth inhibitory activity against Escherichia coli , indicating its potential antibacterial properties [ 34 ]. Additionally, synthetic peptides containing the C-lobe sequence of bovine lactoferrin have exhibited inhibitory effects on bacterial growth, highlighting the antibacterial potential of the C-lobe [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Lactoferrins in Their Interactions with Molecular Targets: A Structure-Based Overview

Piacentini,

Boffi,

Milanetti

2024

Pharmaceuticals

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Lactoferrins and lactoferrin-derived peptides display numerous functions linked to innate immunity in mammalians, spanning from antimicrobial to anti-inflammatory and immunomodulatory actions, and even demonstrate antitumor properties. To date, the proposed mechanisms for their biological actions are varied, although the molecular basis that governs lactoferrin interactions with molecular targets has been clarified only in a limited number of specific cases. However, key in silico methods have recently moved the topic to the fore, thus greatly expanding the possibilities of large-scale investigations on macromolecular interactions involving lactoferrins and their molecular targets. This review aims to summarize the current knowledge on the structural determinants that drive lactoferrin recognition of molecular targets, with primary focus on the mechanisms of activity against bacteria and viruses. The understanding of the structural details of lactoferrins’ interaction with their molecular partners is in fact a crucial goal for the development of novel pharmaceutical products.

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Inhibitory Effects of Synthetic Peptides Containing Bovine Lactoferrin C-lobe Sequence on Bacterial Growth

Cited by 6 publications

References 37 publications

The Impact of Lactoferrin on the Growth of Intestinal Inhabitant Bacteria

The Impact of Lactoferrin on the Growth of Intestinal Inhabitant Bacteria

Heterologous expression of bovine lactoferrin C-lobe in Bacillus subtilis and comparison of its antibacterial activity with N-lobe

Lactoferrins in Their Interactions with Molecular Targets: A Structure-Based Overview

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