Inhibitory effects of sepiapterin on vascular endothelial growth factor-a-induced proliferation and adhesion in human umbilical vein endothelial cells

Kim, Soo Hyeon; Cho, Young‐Rak; Kim, Myoung-Dong; Kim, Hyun Ju; Choi, Shin Wook; Seo, Dong Wan

doi:10.1007/s12272-011-0920-7

Cited by 9 publications

(4 citation statements)

References 31 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the contrary, the elevation of gland number and the reduction of fibrosis area in the endometrium were discovered in the IUA rats treated with si-KDR. Interestingly, there was evidence of a close relationship between VEGF-induced cell proliferation and adhesions and the KDR downstream signaling pathways (23), and our study demonstrated that silencing KDR can contribute to the down-regulation of VEGF, which was consistent with the finding by Aesoy et al (24), indicating that VEGF may interact with KDR in the endometrium of IUA patients. To date, accumulating reports have shown that VEGF can participate in the occurrence and progression of IUA.…”

Section: Discussionsupporting

confidence: 92%

The role of KDR in intrauterine adhesions may involve the TGF-β1/Smads signaling pathway

Chen

et al. 2019

Braz J Med Biol Res

View full text Add to dashboard Cite

The aim of this study was to investigate the role of kinase-insert domain-containing receptor (KDR) in intrauterine adhesions (IUA) and its mechanism. The Case group consisted of 92 patients diagnosed with IUA, and the Control group included 86 patients with uterine septum who had normal endometrium verified with an uteroscope. In addition, 50 rats were randomly assigned into Control, Sham, Model, NC-siRNA, and KDR-siRNA groups. Rats in the Model, NC-siRNA, and KDR-siRNA groups were induced by uterine curettage and lipopolysaccharide (LPS) treatment to establish the IUA model. Then, immunohistochemistry was applied for detection of VEGF and KDR expression, HE staining was used for observation of the endometrial morphology and gland counting, Masson staining for measurement of the degree of endometrial fibrosis, and qRT-PCR and western blot for the expression of KDR, VEGF, MMP-9, as well as TGF-β1/Smads pathway-related proteins. Compared with the Control group, the mRNA and protein expressions of KDR were significantly higher in IUA endometrial tissues, and the expression of KDR was positively correlated to the severity of IUA. In addition, the injection of si-KDR increased the number of endometrial glands, reduced the area of fibrosis, inhibited mRNA and protein expression of KDR and VEGF, up-regulated the expression of MMP-9 and Smad7, and decreased the expression level of TGF-β1, p-Smad2, p-Smad3, and Smad4 in rats with IUA. Highly-expressed KDR was related to patients' severity of IUA, and silencing KDR may prevent the occurrence and development of IUA via TGF-β1/Smads signaling pathway and up-regulating the expression of MMP-9.

show abstract

Section: Discussionsupporting

confidence: 92%

The role of KDR in intrauterine adhesions may involve the TGF-β1/Smads signaling pathway

Chen

et al. 2019

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…Migration assay. Cell migration was quantified in the in vitro wound-healing assay as previously described (23,24). After cells were plated on 48-well plates, and grown to confluence, a single wound was created in the center of the cell monolayer by the gentle removal of the attached cells with a sterile plastic pipette tip.…”

Section: Cellmentioning

confidence: 99%

The in vitro antitumor activity of Siegesbeckia glabrescens against ovarian cancer through suppression of receptor tyrosine kinase expression and the signaling pathways

Cho

Choi²,

Seo

2013

Oncology Reports

Self Cite

View full text Add to dashboard Cite

Siegesbeckia glabrescens (SG) Makino (Compositae) has been used as a traditional medicine for the treatment of a variety of diseases such as allergy, inflammation, acute hepatitis and hypertension. The primary aim of this study was to determine whether the ethanol extract of SG has antitumor activity against ovarian cancer and to identify molecular mechanisms and targets involved in the regulation of cell growth and progression. We demonstrate that SG treatment inhibits proliferation, adhesion, migration and invasion of SKOV-3 human ovarian cancer cells. The anti-proliferative effect of SG on SKOV-3 cells is accompanied by reduced expression of cyclin E and enhanced expression of the cyclin-dependent kinase inhibitor p27(Kip1), leading to inhibition of pRb phosphorylation. We also show that these antitumor activities are found to be mediated through suppression of FAK, ERK, Akt and p70(S6K)-dependent signaling pathways and downregulation of receptor tyrosine kinases such as EGFR, VEGFR-2 and FGFR-1 as well as the cell adhesion molecule N-cadherin. Taken together, our findings suggest further development and evaluation of SG for the treatment of ovarian cancer.

show abstract

“…The cells were photographed and counted. The results (mean ± standard deviation) are presented as the fold-increase of the untreated adherent cells (16).…”

Section: Cellmentioning

confidence: 99%

Knockdown of integrin α3β1 expression induces proliferation and migration of non-small cell lung cancer cells

Yoon¹,

Cho²,

Joo³

et al. 2012

Oncology Reports

Self Cite

View full text Add to dashboard Cite

Abstract. Integrin α3β1 is expressed on many types of cancer cells and can regulate tumor growth and progression. In the present study, we examined the roles and molecular mechanism of integrin α3β1 in modulating cell proliferation and migration of p53-deficient non-small cell lung cancer (NSCLC) cells. Reduced expression of integrin α3 by RNA silencing clearly induces cell proliferation and migration in H1299 cells, compared with those in control cells. Enhanced proliferation in integrin α3-silenced cells is mediated by upregulation and nuclear localization of cyclin-dependent kinases, and these effects require the activation of Akt and ERK as evidenced by treatment with LY294002 and PD98059, respectively. Furthermore, suppression of integrin α3 expression induces the expression of nuclear factor-κB and Bcl-2 as well as epidermal growth factor receptor, which are positively correlated with cell proliferation and survival. In contrast, increase in cell migration of integrin α3-silenced cells is found to be independent of Akt or ERK signaling pathways. Collectively, these findings suggest that integrin α3β1 plays pivotal roles in regulating cell proliferation and migration that enhance the invasive type of p53-deficient NSCLC cells.

show abstract

Inhibitory effects of sepiapterin on vascular endothelial growth factor-a-induced proliferation and adhesion in human umbilical vein endothelial cells

Cited by 9 publications

References 31 publications

The role of KDR in intrauterine adhesions may involve the TGF-β1/Smads signaling pathway

The role of KDR in intrauterine adhesions may involve the TGF-β1/Smads signaling pathway

The in vitro antitumor activity of Siegesbeckia glabrescens against ovarian cancer through suppression of receptor tyrosine kinase expression and the signaling pathways

Knockdown of integrin α3β1 expression induces proliferation and migration of non-small cell lung cancer cells

Contact Info

Product

Resources

About