Inhibitory Effects of Polyphenols on Gastric Injury by <i>Helicobacter pylori</i> VacA Toxin

Yahiro, Kinnosuke; Shirasaka, Daisuke; Tagashira, Motoyuki; Wada, Akihiro; Morinaga, Naoko; Kuroda, Fuminobu; Choi, Oksun; Inoue, Masahito; Aoyama, Nobuo; Ikeda, Mitsuo; Hirayama, Toshiya; Moss, Joel; Noda, Masatoshi

doi:10.1111/j.1523-5378.2005.00315.x

Cited by 45 publications

(37 citation statements)

References 38 publications

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“…In many cases, the antibacterial activity of both herbal extracts and foodstuffs has been associated with their content in phenolic compounds, in particular, flavonoids (11)(12)(13), resveratrol (6), and hydrolyzable tannins (14). The mechanism by which phenolic compounds affect the growth of H. pylori is unknown, but different theories have been proposed, for example, inhibition of the urease activity (15), adhesion to human gastric mucus (10), disintegration of the outer membrane (16), and inhibition of VacA citotoxin activity which causes the development of inflammation and ulceration in patients (17,18).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Activity of Olive Oil Polyphenols against Helicobacter pylori

Romero

Medina

Vargas

et al. 2007

J. Agric. Food Chem.

168

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Helicobacter pylori is linked to a majority of peptic ulcers and to some types of gastric cancer, and resistance of the microorganism to antibiotic treatment is now found worldwide. Virgin olive oil is an unrefined vegetable oil that contains a significant amount of phenolic compounds. Under simulated conditions, we have demonstrated that these substances can diffuse from the oil into the gastric juice and be stable for hours in this acidic environment. In vitro, they exerted a strong bactericidal activity against eight strains of H. pylori, three of them resistant to some antibiotics. Among the phenolic compounds, the dialdehydic form of decarboxymethyl ligstroside aglycon showed the strongest bactericidal effect at a concentration as low as 1.3 μg/mL. Although the experimental conditions are different from other reported works, this bactericidal concentration is much lower than those found for phenolic compounds from tea, wine, and plant extracts. These results open the possibility of considering virgin olive oil a chemopreventive agent for peptic ulcer or gastric cancer, but this bioactivity should be confirmed in vivo in the future. Keywords: Olive oil; phenolic compounds; simulated digestion, Helicobacter pylori; antimicrobial

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Section: Introductionmentioning

confidence: 99%

In Vitro Activity of Olive Oil Polyphenols against Helicobacter pylori

Romero

Medina

Vargas

et al. 2007

J. Agric. Food Chem.

168

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“…We have previously found that polyphenols and polyphenol-rich beverages, such as tea and wine, inhibit VacA activity in vitro [12] , and that some polyphenols can limit both H pylori-and VacA-induced gastric damage in vivo [13] . Also, it has been recently reported that hop bract extract exerts anti-VacA activity in vitro and in vivo [14] . In the present paper we investigated whether red wine and green tea concentrates could influence gastric colonization or gastric pathology in H pylori-infected or VacA-treated mice.…”

Section: Introductionmentioning

confidence: 99%

Red wine and green tea reduceH pylori-orVacA-inducedgastritis in a mouse model

Ruggiero

Rossi²,

Tombola³

et al. 2007

WJG

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AIM:To investigate whether red wine and green tea could exert anti-H pylori or anti-VacA activity in vivo in a mouse model of experimental infection. METHODS:Ethanol-free red wine and green tea concentrates were administered orally as a mixture of the two beverages to H pylori infected mice, or separately to VacA-treated mice. Gastric colonization and gastric inflammation were quantified by microbiological, histopathological, and immunohistochemical analyses. RESULTS:In H pylori -infected mice, the red wine and green tea mixture significantly prevented gastritis and limited the localization of bacteria and VacA to the surface of the gastric epithelium. Similarly, both beverages significantly prevented gastric epithelium damage in VacA-treated mice; green tea, but not red wine, also altered the VacA localization in the gastric epithelium. CONCLUSION:Red wine and green tea are able to prevent H pylori -induced gastric epithelium damage, possibly involving VacA inhibition. This observation s u p p o r t s t h e p o s s i b l e r e l e va n c e o f d i e t o n t h e pathological outcome of H pylori infection.

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“…Also, we have observed the ability of dealcoholized wine and green tea to reduce gastritis in mice experimentally infected by H. pylori (unpublished data). Hop bract extract has been recently reported to exert anti-VacA activity (35). Here, we investigated whether pure polyphenols could influence gastric colonization or gastritis in mice and/or counteract the effects of VacA in vivo.…”

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confidence: 99%

Polyphenols Reduce Gastritis Induced by Helicobacter pylori Infection or VacA Toxin Administration in Mice

Ruggiero

Tombola

Rossi

et al. 2006

Antimicrob Agents Chemother

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Helicobacter pylori colonizes the human gastric mucosa, causing inflammation that leads to atrophic gastritis, and it can cause peptic ulcer and gastric cancer. We show that polyphenol administration to mice experimentally infected by H. pylori or treated with VacA toxin can limit gastric epithelium damage, an effect that may be linked to VacA inhibition.Helicobacter pylori chronically infects the gastric mucosa of Ͼ50% of the human population, causing gastritis. The infection can lead to the development of peptic ulcer (27) and gastric mucosa-associated lymphoid tissue lymphoma (6) and increases the risk of gastric cancer in humans (8,11,31). It has been proven that H. pylori infection can cause gastric cancer in animals (9, 33). In both humans and animals, the gastric pathology depends on the virulence of the H. pylori infecting strain and on the genetic background of the host (4, 10, 22). In humans, lifestyle is also relevant (13, 26). The current antibiotic-based therapies are generally effective but can fail due to antibiotic resistance or lack of patient compliance. Thus, there is a continuous effort to develop new tools to fight against this pathogen. In particular, plant extracts or plant-derived substances have been investigated for anti-H. pylori activity in vitro (1,2,14,15,18,20,24) and in vivo (12,19,28).H. pylori expresses several factors that allow host stomach colonization and can play a role in pathogenesis. One of the most important factors is the vacuolating cytotoxin VacA (3, 29). We have already reported that polyphenols inhibit VacAinduced ion/urea conduction and cell vacuolation (30). Also, we have observed the ability of dealcoholized wine and green tea to reduce gastritis in mice experimentally infected by H. pylori (unpublished data). Hop bract extract has been recently reported to exert anti-VacA activity (35). Here, we investigated whether pure polyphenols could influence gastric colonization or gastritis in mice and/or counteract the effects of VacA in vivo.Animal experiments were done in compliance with current law. Data were evaluated by one-tailed Mann-Whitney U test, with P values of Ͻ0.05 considered significant.H. pylori strain SPM 326 type I, expressing the s1/m1 VacA isoform, was used to intragastrically infect specific-pathogenfree CD1 mice (Charles River, Calco, Italy), a well-established H. pylori infection model (17, 32). Noninfected controls received saline only. Starting 24 h before the infection, the animals had free access to drinking water containing 1% glucose and a mixture of 1.5 mg/ml each of tannic acid (average molecular weight, 1,701.20) and n-propyl gallate (Sigma-Aldrich, Milan, Italy) (TAϩNPG). TA and NPG were selected because of their previously shown anti-VacA activity in vitro (30), their water solubility, and the relative abundance of tannins and gallates in common beverages, such as wine and tea. Also, TA is a GRAS (generally regarded as safe) food additive. Based on previous results obtained with wine (unpublished data), the polyphenol concentration cho...

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Inhibitory Effects of Polyphenols on Gastric Injury by Helicobacter pylori VacA Toxin

Abstract: HBT may suppress the development of inflammation and ulceration caused by H. pylori VacA, suggesting that HBT may be useful as a new type of therapeutic agent for the prevention of gastric ulcer and inflammation caused by VacA.

Cited by 45 publications

References 38 publications

In Vitro Activity of Olive Oil Polyphenols against Helicobacter pylori

In Vitro Activity of Olive Oil Polyphenols against Helicobacter pylori

Red wine and green tea reduceH pylori-orVacA-inducedgastritis in a mouse model

Polyphenols Reduce Gastritis Induced by Helicobacter pylori Infection or VacA Toxin Administration in Mice

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