Inhibitory effects of phenolic compounds of rice straw formed by saccharification during ethanol fermentation by Pichia stipitis

Wang, Xiahui; Tsang, Yiu Fai; Li, Yuhao; Ma, Xinxue; Cui, Shouqing; Zhang, Tian-Ao; Hu, Jiajun; Gao, Min-Tian

doi:10.1016/j.biortech.2017.08.096

Cited by 45 publications

(17 citation statements)

References 32 publications

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“…Additionally, genes upregulated in the presence of aromatics were enriched in GO terms related to lipase and endopeptidase activity (Figure 4). Upregulation of genes linked to lipid and amino acid metabolism (Supplementary Table 1) could indicate that the fungus is dealing with an increased lipid and protein turnover e.g., due to aromatics-inflicted injuries on cellular membranes (Fitzgerald et al, 2004; Gu et al, 2015; Wu et al, 2016; Wang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

The White-Rot Basidiomycete Dichomitus squalens Shows Highly Specific Transcriptional Response to Lignocellulose-Related Aromatic Compounds

Kowalczyk

Peng

Pawlowski

et al. 2019

Front. Bioeng. Biotechnol.

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Lignocellulosic plant biomass is an important feedstock for bio-based economy. In particular, it is an abundant renewable source of aromatic compounds, which are present as part of lignin, as side-groups of xylan and pectin, and in other forms, such as tannins. As filamentous fungi are the main organisms that modify and degrade lignocellulose, they have developed a versatile metabolism to convert the aromatic compounds that are toxic at relatively low concentrations to less toxic ones. During this process, fungi form metabolites some of which represent high-value platform chemicals or important chemical building blocks, such as benzoic, vanillic, and protocatechuic acid. Especially basidiomycete white-rot fungi with unique ability to degrade the recalcitrant lignin polymer are expected to perform highly efficient enzymatic conversions of aromatic compounds, thus having huge potential for biotechnological exploitation. However, the aromatic metabolism of basidiomycete fungi is poorly studied and knowledge on them is based on the combined results of studies in variety of species, leaving the overall picture in each organism unclear. Dichomitus squalens is an efficiently wood-degrading white-rot basidiomycete that produces a diverse set of extracellular enzymes targeted for lignocellulose degradation, including oxidative enzymes that act on lignin. Our recent study showed that several intra- and extracellular aromatic compounds were produced when D. squalens was cultivated on spruce wood, indicating also versatile aromatic metabolic abilities for this species. In order to provide the first molecular level systematic insight into the conversion of plant biomass derived aromatic compounds by basidiomycete fungi, we analyzed the transcriptomes of D. squalens when grown with 10 different lignocellulose-related aromatic monomers. Significant differences for example with respect to the expression of lignocellulose degradation related genes, but also putative genes encoding transporters and catabolic pathway genes were observed between the cultivations supplemented with the different aromatic compounds. The results demonstrate that the transcriptional response of D. squalens is highly dependent on the specific aromatic compounds present suggesting that instead of a common regulatory system, fine-tuned regulation is needed for aromatic metabolism.

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Section: Discussionmentioning

confidence: 99%

The White-Rot Basidiomycete Dichomitus squalens Shows Highly Specific Transcriptional Response to Lignocellulose-Related Aromatic Compounds

Kowalczyk

Peng

Pawlowski

et al. 2019

Front. Bioeng. Biotechnol.

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“…Moreover, the presence of dissolved inorganic compounds and toxic components should be tested and avoided through a detoxification stage. Finally, Moreover, the pretreatment process does not have a high lignin solubilization due to the adverse effects of phenolic compound in fermentation processes [37].…”

Section: Sugars and Inhibitory Compounds In Hydrolysatesmentioning

confidence: 99%

Effect of dilute sulfuric acid pretreatment on the physicochemical properties and enzymatic hydrolysis of coffee cut-stems

Solarte-Toro

Chacón-Perez

Piedrahita-Rodríguez

et al. 2020

Energy

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Coffee-cut stems are a potential fermentable sugars source, which can be upgraded in different valueadded products and energy vectors. Nevertheless, there are few reports focused on the acid pretreatment and saccharification processes. Thus, this paper evaluates the effect of the acid pretreatment and saccharification conditions of coffee cut-stems to find the highest sugar yield. Thereafter, the influence of the residence time in the acid pretreatment and the b-glucosidase supplementation in the saccharification process were analyzed. The combined severity factor and crystallinity index were used as metrics to evaluate both processes. In all assays, an increase in the crystallinity index was observed. Furthermore, a nonlinear trend of the combined severity factor respect to the residence time in the acid pretreatment was evidenced. The highest sugar yield was 66.75% with a combined severity factor of 1.84. The better saccharification process was achieved at combined severity factor of 2.01 with a digestibility of 43%. The addition of b-glucosidase in the enzymatic hydrolysis allows increasing the value to 69.07%. Hence, low temperatures, acid concentrations, and the b-glucosidase supplementation allows obtaining a high sugar yield from coffee cut stems.

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“…The effect of the recovery of phenolic acids on the cost for ethanol production was discussed, coming the conclusion that the recovery of phenolic acids could significantly reduce the total cost for ethanol production. To lower the inhibitory effect of phenolic acids on yeast growth, an extraction process was needed [ 13 ]. Most phenolic acids are ether-linked to lignin and polysaccharides, and the linked phenolic acid could be released by the aids of acid and alkali.…”

Section: Introductionmentioning

confidence: 99%

Tapping the Bioactivity Potential of Residual Stream from Its Pretreatments May Be a Green Strategy for Low-Cost Bioconversion of Rice Straw

Chen

Wang

Xue

et al. 2018

Appl Biochem Biotechnol

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In this study, it was found that the residual stream from pretreatments of rice straw exhibited high antioxidant activity. Assays based on the Folin–Ciocalteu colorimetric method confirmed that the residual stream contained large amounts of phenolic compounds. Three antioxidant assays were employed to evaluate the bioactivity of the residual stream. Strong linear correlations existed among the release of phenolic compounds, saccharification efficiency, and antioxidant activity. The alkaline pretreatment provided a much greater release of phenolic compounds, especially phenolic acids, compared to the acid pretreatment, and consequently, it had stronger linear correlations than the acid pretreatment. Antibacterial experiments demonstrated the ability of the phenolic compounds in the residual stream to inhibit the growth of microorganisms, indicating the potential of these compounds as antimicrobial agents. To discuss the possibility of the co-production of antimicrobial agents and biofuels/biochemicals, both acid and alkaline pretreatments were optimized using response surface methodology. Under the optimal conditions, 285.7 g glucose could be produced from 1 kg rice straw with the co-production of 3.84 g FA and 6.98 g p-CA after alkaline pretreatment. These results show that the recovery of phenolic compounds from the residual stream could be a green strategy for the low-cost bioconversion of rice straw.Electronic supplementary materialThe online version of this article (10.1007/s12010-018-2751-1) contains supplementary material, which is available to authorized users.

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Inhibitory effects of phenolic compounds of rice straw formed by saccharification during ethanol fermentation by Pichia stipitis

Cited by 45 publications

References 32 publications

The White-Rot Basidiomycete Dichomitus squalens Shows Highly Specific Transcriptional Response to Lignocellulose-Related Aromatic Compounds

The White-Rot Basidiomycete Dichomitus squalens Shows Highly Specific Transcriptional Response to Lignocellulose-Related Aromatic Compounds

Effect of dilute sulfuric acid pretreatment on the physicochemical properties and enzymatic hydrolysis of coffee cut-stems

Tapping the Bioactivity Potential of Residual Stream from Its Pretreatments May Be a Green Strategy for Low-Cost Bioconversion of Rice Straw

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