Inhibitory effects of oxali-Platin as a chemotherapeutic drug on the function and structure of bovine liver catalase

Abazari, Omid; Divsalar, Adeleh; Ghobadi, Roohollah

doi:10.1080/07391102.2019.1581088

Cited by 23 publications

(7 citation statements)

References 15 publications

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“…Conventional systems for cancer therapy such as chemotherapy have been currently employed for treating malignant breast cancer. Platinum drugs such as cisplatin (also called cis -diammine-dichloro-platinum (II)) and its analogs are the most effective anti-tumor compounds which routinely used in clinics for the different types of human solid tumors remedy via multiple mechanisms ( 2 , 3 ). The anti-tumor activity of cisplatin is mediated by its ability to DNA damage in tumor cells and interfering with its repair mechanism ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

The Synergistic Combination of Cisplatin and Piperine Induces Apoptosis in MCF-7 Cell Line

Fattah¹,

Morovati²,

Niknam³

et al. 2021

ijph

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Background: Piperine is a natural compound obtained from the Piper nigrum that exhibits anti-proliferative and anti-cancer activity in cancer cell lines. We analyzed the cytotoxic effect of piperine combined with cisplatin compound in the human MCF-7 breast cancer cell line and the underlying mechanism. Methods: The present in vitro study was performed on MCF-7 cell line in Jahrom University of Medical Sciences between, Jahrom, Iran from 2016 to 2017. Cultured MCF-7 cells were seeded into four groups: a control group (untreated group), a group treated with cisplatin, a group treated with piperine and a group treated with cisplatin and piperine. Cell viability was analyzed using the MTT assay method. Flow c-ytometric analysis was investigated for apoptosis. The mRNA and protein expression of the apoptotic regulators p53, Bcl-2, Bax, caspase 3 and caspase 9 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting analysis. Results: Piperine (20 and 30 µM) in combination with cisplatin (5, 10 and 15 µM) for 24 h synergistically inhibited cell viability of MCF-7 breast cancer cells more than piperine and cisplatin used alone. Synergistic antibreast cancer activities cisplatin (5 µM) and piperine (20 µM) were via inducing apoptosis. Piperine (20 µM) and cisplatin (5 µM) for 24 h induce apoptosis strongly through reduction of Bcl-2 and increase of caspase 3, p53, caspase 9, and Bax. Conclusion: Piperine in combination with cisplatin could trigger p53-mediated apoptosis more effective than cisplatin alone in MCF-7 breast cancer cells, reducing the toxic dose of cisplatin used in cancer chemotherapy.

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Section: Introductionmentioning

confidence: 99%

The Synergistic Combination of Cisplatin and Piperine Induces Apoptosis in MCF-7 Cell Line

Fattah¹,

Morovati²,

Niknam³

et al. 2021

ijph

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“…2'-deoxycytidine-5-Aza or Decitabine is an epigenetic medicine, a decisive hypomethylating factor, and usually used to demethylate some gene's promoter (12,13). Various scientific studies determine that Decitabine changes the gene expression through demethylation of the gene's promoter and independently from DNA demethylation (14,15). Different studies showed that unusual DNA methylation and histone deacetylation are associated with tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Decitabine Effects on HDAC3 and HDAC7 mRNA Expression in NALM-6 and HL-60 Cancer Cell Lines

et al. 2021

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Background: Decitabine is a potent anticancer hypomethylating agent and changes the gene expression through the gene's promoter demethylation and also independently from DNA demethylation. So, the present study was designed to distinguish whether Decitabine, in addition to inhibitory effects on DNA methyltransferase, can change HDAC3 and HDAC7 mRNA expression in NALM-6 and HL-60 cancer cell lines. Methods: HL-60, NALM-6, and normal cells were cultured, and the Decitabine treatment dose was obtained (1 µM) through the MTT assay. Finally, HDAC3 and HDAC7 mRNA expression were measured by Real-Time PCR in HL-60 and NALM-6 cancerous cells before and after treatment. Furthermore, HDAC3 and HDAC7 mRNA expression in untreated HL-60 and NALM-6 cancerous cells were compared to normal cells. Results: Our results revealed that the expression of HDAC3 and HDAC7 in HL-60 and NALM-6 cells increases as compared to normal cells. After treatment of HL-60 and NALM-6 cells with Decitabine, HDAC3, and HDAC7 mRNA expression were decreased significantly. Conclusions: Our data confirmed that the effects of Decitabine are not limited to direct hypomethylation of DNMTs, but it can indirectly affect other epigenetic factors, such as HDACs activity, through converging pathways.

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“…Each connexon contains six transmembrane protein monomers, which are called connexin (Cx). According to their molecular weight, Cxs are encoded by a conserved gene family with 21 different members in mammals, which are expressed in a cell-dependent pathway (5,6). The Cx26, Cx32, and Cx43, the building units of liver GJ, are expressed in the mature hepatocytes and Kupffer cells (5).…”

Section: Introductionmentioning

confidence: 99%

Alteration of connexins gene expression by silibinin during hepatic warm ischemia-reperfusion injury in the rat model

Ghobadi

Mousavi

Mosavi

et al. 2021

ircmj

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Background: Ischemia-reperfusion injury (IRI) is an injurious phenomenon that is the primary determinant of liver dysfunction after surgery and transplantation. The present evidence demonstrated that connexin 43 (Cx43), Cx32, and Cx26 are the essential gap junction proteins involved in the liver IRI. This study aimed to characterize the beneficial effects of silibinin on Cx43, Cx32, and Cx26 gene expression during warm hepatic ischemia-reperfusion (IR). Materials and Methods: A total of 32 male Wistar rats weighing 250-300 g were randomly divided into four equal groups of eight animals in each group as follows: 1) control group (laparotomy+normal saline), 2) laparotomy+silibinin (30 mg/kg) (SILI), 3) liver IR procedure+normal saline (IR), and 4) liver IR procedure+silibinin (30 mg/kg) (IR+SILI). After 1 h of ischemia followed by 3 h of reperfusion, blood samples and tissue sections were gathered to assess the serum liver markers and evaluate the liver histological changes as well as gene expression, respectively. Results: The obtained data proved no considerable differences between control and SILI groups in all experiments. Furthermore, the gene expression of Cx26, Cx32, and Cx43 was significantly induced in the IR group, compared to the control group. Silibinin markedly reduced Cx26 and Cx32 mRNA expression, whereas increased Cx43 mRNA expression. Moreover, serum alanine aminotransferase and aspartate aminotransferase levels were markedly elevated in the IR group (P<0.001), compared to the control group. However, in the IR+SILI group, silibinin could significantly decline these elevations, compared to the IR group. In addition, silibinin diminished hepatic tissue damages during IR. Conclusion: Silibinin could attenuate liver injury through better cell-to-cell communication via lowering Cx32 and Cx26, as well as increasing Cx43 gene expression, respectively.

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Inhibitory effects of oxali-Platin as a chemotherapeutic drug on the function and structure of bovine liver catalase

Cited by 23 publications

References 15 publications

The Synergistic Combination of Cisplatin and Piperine Induces Apoptosis in MCF-7 Cell Line

The Synergistic Combination of Cisplatin and Piperine Induces Apoptosis in MCF-7 Cell Line

Investigation of Decitabine Effects on HDAC3 and HDAC7 mRNA Expression in NALM-6 and HL-60 Cancer Cell Lines

Alteration of connexins gene expression by silibinin during hepatic warm ischemia-reperfusion injury in the rat model

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