Inhibitory Effects of Opuntia humifusa on 7, 12-Dimethyl-benz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate Induced Two-stage Skin Carcinogenesis

Abstract

Opuntia humifusa, member of the Cactaceae family, was previously demonstrated to have radical scavenging, anti-inflammatory and anti-proliferative effects in in vitro models. It was suggested that O. humifusa could function in the prevention of carcinogenesis. To investigate the in vivo chemopreventive effect of O. humifusa, mice were fed a diet containing either 1% or 3% following 7, 12-dimethylbenz[a] anthracene (DMBA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) induction of skin carcinogenesis. Significant decrease in the numbers of papilloma and epidermal hyperplasia were observed in mice fed with O. humifusa, compared to the control group. O. humifusa also upregulated high total antioxidant capacity and level of phase II detoxifying enzyme such as superoxide dismutase and glutathione S-transferase activity in the skin. Lipid peroxidation activity level was measured in skin cytosol and significantly inhibited in 3% OH fed group compared to the control group. These results suggest that O. humifusa exerts chemopreventive effects on chemical carcinogenesis in mouse skin and that prevention effects are associated with reduction of oxidative stress via the modulation of cutaneous lipid peroxidation, enhancing of total antioxidant capacity especially in phase II detoxifying enzyme system and partial apoptotic influence.

Opuntiaspp.: Characterization and Benefits in Chronic Diseases

“…More studies are needed to confirm the protective effects of Opuntia spp., testing those compounds in a more physiological way or for instance by oral route that will take into account the digestibility and bioavailability of such compounds. In this spirit, O. humifusa fruit lyophilized powder given in the pelleted diet was reported to be protective in two different animal models of skin carcinogenesis, together with a reduction of skin lipid peroxidation and skin inflammation [122, 123]. …”

Efecto quimioprotector del extracto alcaloideo de Melocactus bellavistensis (CACTUS GLOBOSO) sobre el cáncer de colon inducido con 1,2-dimetilhidrazina en ratas

“…Por otro lado, en la familia Cactaceae se han reportado varios estudios in vivo de los efectos anticancerígenos, entre ellos el del extracto acuoso de la penca de Opuntia ficusindica, que revierte el efecto del cáncer hepático inducido por aflatoxina B1 en ratones machos BALB/c (28) ; el del extracto acuoso de la pera de Opuntia ficus-indica, el cual suprime el crecimiento de tumores en ratones hembra BALC/c xenoinjertado con células SKOV3 relacionadas con cáncer cervical (29) . En otro estudio se reporta que el polvo del fruto del cactus de Opuntia humifusa disminuye el número de papilomas y la hiperplasia epidermal en ratones hembra BALC/c tratadas con 7, 12-Dimetilbenz[a] antraceno (30) . Todo esto avala el potencial de la familia Cactaceae como fuente de anticancerígenos, entre ellos al Melocactus bellavistensis.…”

Fermented Prunus mume with Probiotics Inhibits 7,12-Dimethylbenz[a]anthracene and 12-O-Tetradecanoyl phorbol-13-acetate Induced Skin Carcinogenesis through Alleviation of Oxidative Stress

“…Therefore, a direct relationship has been reported between antioxidant activity and anticancer activity of these compounds involving the risk of oxidative damage-induced skin carcinogenesis (Ben-Dor et al, 2005;Kamaraj et al, 2009). Previously, we have shown that O.humifusa, a member of the Cactaceae family, has the ability to inhibit 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate induced skin carcinogenesis in mice via the reduction of oxidative stress (Lee et al, 2012). Thus, the present study was designed to investigate the inhibitory effect of fermented Maesil with probiotics (FM) through its anti-oxidative effect in DOI:http://dx.doi.org/10.7314/APJCP.2013.14.5.2973 Inhibitory Effects of Prunus Mume on Skin Carcinogenesis skin carcinogenesis.…”

“…The experimental groups received the same extruded dry rodent feed supplemented with either 1% (w/w) fermented Maesil (1% FM group) or 2% (w/w) fermented Maesil (2% FM group). Experimental diets containing 1% and 2% fermented maesil were prepared as previously described (Lee et al, 2012). For carcinogenesis studies, supplemented diet containing fermented Maesil was provided to the mice starting 3 weeks before the initiation of DMBA treatment.…”