Inhibitory effects of miR‑26b‑5p on thyroid cancer

Abstract

In order to examine the inhibitory effects of microRNA (miR)-26b-5p on thyroid cancer (TC), the clinicopathological features and pathological tissues of 67 patients were collected. The expression levels of miR-26b-5p were detected in TC and paracarcinoma tissues by quantitative polymerase chain reaction, and the association between miR-26b-5p expression and the clinicopathological features of the patients was analyzed using t-test or one-way analysis of variance. In addition, B-CPAP TC cells were infected with a lentivirus to induce miR-26b-5p overexpression and proliferation was detected by Cell Counting kit-8. Subsequently, migration and invasion were detected by Transwell and Matrigel assays, respectively, and the molecular mechanism of action was investigated by western blotting. The results demonstrated that the expression levels of miR-26b-5p were significantly lower in TC tissues compared with paracarcinoma tissues (P<0.01), and miR-26b-5p was associated with lymph node metastasis (P<0.05). In addition, overexpression of miR-26b-5p inhibited the proliferation, invasion and migration of B-CPAP cells. Western blot analysis demonstrated that the protein expression levels of phosphorylated glycogen synthase kinase-3β (pGsk-3β) were decreased, and the expression of β-catenin was decreased in B-CPAP cells overexpressing miR-26b-5p. These results demonstrated that miR-26b-5p may exert antitumor activity. In addition, at the molecular level, these effects may be associated with the Gsk-3β/β-catenin pathway.

Lymphocyte to monocyte ratio predicts survival and is epigenetically linked to miR-222-3p and miR-26b-5p in diffuse large B cell lymphoma

“…We also did not find any significance between miR-26b-5p and the clinical data of our studied cases. Our study is consistent with previous studies, which indicated no significance between miR-26b-5p and clinical data including age, sex, tumour stage 55 , grade, and survival 16 . On the contrary, other studies showed a significant correlation between miR-26b-5p and both survival in HCC 18 and clinical stage in thyroid cancer 17 .…”

Long non-coding RNA HAGLROS facilitates tumorigenesis and progression in hepatocellular carcinoma by sponging miR-26b-5p to up-regulate karyopherin α2 (KPNA2) and inactivate p53 signaling

“…Subsequent experiments confirmed that miR-26b-5p was a direct target of HAGLROS. MiR-26b-5p has been demonstrated as an oncogenic driver in some types of tumors, including thyroid cancer [ 31 ], bladder cancer [ 32 ], multiple myeloma [ 33 ] and tongue squamous cell carcinoma [ 34 , 35 ]. What is more, miR-26b-5p was previously revealed to be down-regulated in HCC.…”

Dicing the Disease with Dicer: The Implications of Dicer Ribonuclease in Human Pathologies

“…The alterations of Dicer levels and the consequent aberrant expression of specific miRNAs, with a prominent role in these tissues, may be driving this phenotype. Particularly, miR-21 [ 63 ] and miR-4661-5p [ 64 ] have been found to act as oncogenic miRNAs (onco-miRs) in colorectal cancer and hepatocellular carcinoma, respectively, whereas miR-124-5p [ 65 ] and miR-26b-5p [ 66 ] seem to protect against to colorectal and thyroid cancer, respectively.…”