Inhibitory Effects of Methylglyoxal on Light-Induced Stomatal Opening and Inward K<sup>+</sup>Channel Activity in<i>Arabidopsis</i>

Hoque, Tahsina Sharmin; Okuma, Eiji; Uraji, Misugi; Furuichi, Teiichi; Sasaki, Takayuki; Hoque, Anamul; Nakamura, Yoshimasa; Murata, Yoshiyuki

doi:10.1271/bbb.110885

Cited by 37 publications

(21 citation statements)

References 16 publications

(13 reference statements)

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“…To investigate the mode of action of MG in stomatal guard cell signal transduction, Hoque et al (2012a , c ) investigated stomatal movement in Arabidopsis treated with different concentrations of MG. They found that at concentrations of MG up to 1 mM, MG behaved like a signal molecule as it induced stomatal closure, in a dose-dependant and reversible manner, without reducing the viability of guard cells.…”

Section: Mg As a Signaling Molecule In Plants Under Stressmentioning

confidence: 99%

“…Because of the number of metabolic pathways involved, and the compexity of their regulation, it is often difficult for researchers to identify the major regulatory components involved in the abiotic stress responses of plants ( Sharma et al, 2013 ). Plants subjected to stress often produce toxic aldehydes ( Hoque et al, 2012a , b , c ; Hoque M.A. et al, 2012 ; Mano, 2012 ), of which methylglyoxal (CH 3 COCHO; MG) is the most ubiquitous.…”

Section: Introductionmentioning

confidence: 99%

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Methylglyoxal: An Emerging Signaling Molecule in Plant Abiotic Stress Responses and Tolerance

et al. 2016

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The oxygenated short aldehyde methylglyoxal (MG) is produced in plants as a by-product of a number of metabolic reactions, including elimination of phosphate groups from glycolysis intermediates dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. MG is mostly detoxified by the combined actions of the enzymes glyoxalase I and glyoxalase II that together with glutathione make up the glyoxalase system. Under normal growth conditions, basal levels of MG remain low in plants; however, when plants are exposed to abiotic stress, MG can accumulate to much higher levels. Stress-induced MG functions as a toxic molecule, inhibiting different developmental processes, including seed germination, photosynthesis and root growth, whereas MG, at low levels, acts as an important signaling molecule, involved in regulating diverse events, such as cell proliferation and survival, control of the redox status of cells, and many other aspects of general metabolism and cellular homeostases. MG can modulate plant stress responses by regulating stomatal opening and closure, the production of reactive oxygen species, cytosolic calcium ion concentrations, the activation of inward rectifying potassium channels and the expression of many stress-responsive genes. MG appears to play important roles in signal transduction by transmitting and amplifying cellular signals and functions that promote adaptation of plants growing under adverse environmental conditions. Thus, MG is now considered as a potential biochemical marker for plant abiotic stress tolerance, and is receiving considerable attention by the scientific community. In this review, we will summarize recent findings regarding MG metabolism in plants under abiotic stress, and evaluate the concept of MG signaling. In addition, we will demonstrate the importance of giving consideration to MG metabolism and the glyoxalase system, when investigating plant adaptation and responses to various environmental stresses.

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Section: Mg As a Signaling Molecule In Plants Under Stressmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Methylglyoxal: An Emerging Signaling Molecule in Plant Abiotic Stress Responses and Tolerance

et al. 2016

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“…MG induces stomatal closure by inducing ROS accumulation in the guard cells via the Ca 2+ -dependent pathway and inhibits light-induced stomatal opening by inhibiting K + influx into guard cells, which happens by a modification of the C-terminal region of the KAT1 potassium channel [75]. Data from our studies on pod development in glyoxalase I suppressed transgenic Brassica lines suggest that MG could work in a non-cell-autonomous manner to bring about developmental changes.…”

Section: Functional Roles Of Glyoxalases In Plants: From the Past mentioning

confidence: 99%

Glyoxalase Goes Green: The Expanding Roles of Glyoxalase in Plants

Sankaranarayanan

Jamshed

Abhinandan

et al. 2017

IJMS

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The ubiquitous glyoxalase enzymatic pathway is involved in the detoxification of methylglyoxal (MG), a cytotoxic byproduct of glycolysis. The glyoxalase system has been more extensively studied in animals versus plants. Plant glyoxalases have been primarily associated with stress responses and their overexpression is known to impart tolerance to various abiotic stresses. In plants, glyoxalases exist as multigene families, and new roles for glyoxalases in various developmental and signaling pathways have started to emerge. Glyoxalase-based MG detoxification has now been shown to be important for pollination responses. During self-incompatibility response in Brassicaceae, MG is required to target compatibility factors for proteasomal degradation, while accumulation of glyoxalase leads to MG detoxification and efficient pollination. In this review, we discuss the importance of glyoxalase systems and their emerging biological roles in plants.

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“…For expression in oocytes, cRNAs were synthesized using a mMESSAGE mMACHINE ® T7 transcription kit with cap analog (Ambion, Austin, TX) from linearized pNB1u-KAT1plasmid DNA as described previously 18) with modification. Xenopus oocytes were defolliculated using collagenase and microinjected with 50 ng of cRNAs using a microinjector (model Nanoject II; Drummond Scientific, Broomall, PA) after 3 d of incubation in ND96(+)containing 94 mM NaCl, 2 mM KCl, 1 mM CaCl 2 .2H 2 O, 1 mM MgCl 2 .6H 2 O, 5 mM HEPES (pH 7.5), and 50 μg/mL gentamicin at 18°C.…”

Section: Methodsmentioning

confidence: 99%

Inhibition by acrolein of light-induced stomatal opening through inhibition of inward-rectifying potassium channels in Arabidopsis thaliana

Islam

Matsushima

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Acrolein is a reactive α,β-unsaturated aldehyde derived from lipid peroxides, which are produced in plants under a variety of stress. We investigated effects of acrolein on light-induced stomatal opening using Arabidopsis thaliana. Acrolein inhibited light-induced stomatal opening in a dose-dependent manner. Acrolein at 100 μM inhibited plasma membrane inward-rectifying potassium (Kin) channels in guard cells. Acrolein at 100 μM inhibited Kin channel KAT1 expressed in a heterologous system using Xenopus leaves oocytes. These results suggest that acrolein inhibits light-induced stomatal opening through inhibition of Kin channels in guard cells.

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Inhibitory Effects of Methylglyoxal on Light-Induced Stomatal Opening and Inward K⁺Channel Activity inArabidopsis

Cited by 37 publications

References 16 publications

Methylglyoxal: An Emerging Signaling Molecule in Plant Abiotic Stress Responses and Tolerance

Methylglyoxal: An Emerging Signaling Molecule in Plant Abiotic Stress Responses and Tolerance

Glyoxalase Goes Green: The Expanding Roles of Glyoxalase in Plants

Inhibition by acrolein of light-induced stomatal opening through inhibition of inward-rectifying potassium channels in Arabidopsis thaliana

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Inhibitory Effects of Methylglyoxal on Light-Induced Stomatal Opening and Inward K+Channel Activity inArabidopsis

Cited by 37 publications

References 16 publications

Methylglyoxal: An Emerging Signaling Molecule in Plant Abiotic Stress Responses and Tolerance

Methylglyoxal: An Emerging Signaling Molecule in Plant Abiotic Stress Responses and Tolerance

Glyoxalase Goes Green: The Expanding Roles of Glyoxalase in Plants

Inhibition by acrolein of light-induced stomatal opening through inhibition of inward-rectifying potassium channels in Arabidopsis thaliana

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Inhibitory Effects of Methylglyoxal on Light-Induced Stomatal Opening and Inward K⁺Channel Activity inArabidopsis