Inhibitory effects of methotrexate on spontaneous motility and Cajal-like type of tubal interstitial cells in rabbit oviduct

Yang, Xiao‐Jun; Wei, Wei; Zhao, Jing; Zheng, Fangfang

doi:10.1016/j.fertnstert.2012.04.013

Cited by 6 publications

(2 citation statements)

References 38 publications

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“…Previously, we reported that ICLC was decreased in women with EMs and tubal ectopic pregnancy . In addition, decrease of this kind of interstitial cells was associated with attenuated rabbit oviduct motility . Nevertheless, the exact ultrastructural alterations of TCs population in EMs‐affected oviduct tissue remain unclear.…”

Section: Introductionmentioning

confidence: 93%

Telocytes damage in endometriosis‐affected rat oviduct and potential impact on fertility

Yang

Liu

et al. 2014

J Cellular Molecular Medi

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Women with endometriosis (EMs) have unexplained infertility. The recently identified telocytes (TCs) might participate in the maintenance of structural and functional integrity of oviduct tissue, but so far the involvement of TCs in EMs-affected oviduct tissue and potential impact on fertility capacity remain unknown. By an integrated technique of haematoxylin and eosin staining, in situ immunohistochemistry and double-labelled immunofluorescence staining and electron microscopy approach, TCs were studied in the autotransplantation Sprague–Dawley rat model of EMs-affected oviduct tissue and in sham control, respectively, together with determination of iNOS, COX-2, LPO and estradiol. TCs were found in perivascular connective tissue and smooth muscle bundles in sham oviduct, with typical ultrastructural features (a slender piriform/spindle/triangular cell body, and one or more extremely long prolongations, emerged from cell bodies and extend to various directions), and specific immunophenotype of CD34-positive/vimentin-positive/c-kit-negative. However, in EMs-affected oviduct tissue (grade III), extensive ultrastructural damage (degeneration, discontinue, dissolution and destruction), significant decrease or loss of TCs and interstitial fibrosis were observed, together with elevated level of iNOS, COX-2, LPO and estradiol, thus suggestive of inflammation and ischaemia-induced TCs damage. Based on TCs distribution and intercellular connections, we proposed that such damage might be involved in structural and functional abnormalities of oviduct, such as attenuated intercellular signalling and oviduct contractility, impaired immunoregulation and stem cell-mediated tissue repair, 3-D interstitial architectural derangement and tissue fibrosis. Therefore, TCs damage might provide a new explanation and potential target for EMs-induced tubal damage and fertility disorders.

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Section: Introductionmentioning

confidence: 93%

Telocytes damage in endometriosis‐affected rat oviduct and potential impact on fertility

Yang

Liu

et al. 2014

J Cellular Molecular Medi

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“…According to the literature, c-kit, vimentin, CD34, and PDGFRα were used for the primary identification of tubal TCs [24][25][26][27]. Several studies have reported a role of TCs in immune surveillance or in morphogenetic bioelectrical signaling [26,28,29].…”

Section: Remaining Zone Beneath Serosa Not Assessedmentioning

confidence: 99%

Oviductal Telocytes in Patients with Uterine Myoma

Aleksandrovych

Wrona²,

Bereza

et al. 2021

Biomedicines

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Tubal factor infertility occurs in 30–35% of infertile pairs and may be caused by impaired muscular contractility and ciliary beating as well as immunological imbalance and chronic inflammation. Newly discovered telocytes (TCs) have a wide palette of features, which play a role in oviduct physiology. We have observed tissue samples from human fallopian tubes in patients with and without uterine myoma by immunolabelling. According to the immunohistochemical co-expression of markers, it has been determined that TCs are engaged in a wide range of physiological processes, including local innervation, sensitivity to hypoxia, regulation of calcium, and sex steroid hormones balances. Due to the proximity of NOS- and ChAT-positive nerve fibers and the expression of ion channels markers, tubal TCs might be considered conductor cells. Additionally, their integration in contractions and cilia physiology in the context of fertility has been revealed. We have observed the difference in telocytes expression in the human oviduct between groups of patients and attempted to describe this population of cells specifically in the case of infertility development, a clinically relevant avenue for further studies.

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Immunohistochemistry of Telocytes in the Uterus and Fallopian Tubes

Creţoiu

2016

Advances in Experimental Medicine and Biology

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The seminal work of Popescu and colleagues first demonstrated the existence of a new cell type - the telocytes. We were among the first who reported the presence of such cells in the female genital tract and performed TEM examinations, as well as immunohistochemical staining in the attempt to find a specific marker. Telocytes from rat and from the human uterus and from human fallopian tube were extensively investigated initially by comparison with interstitial cells of Cajal. Progress in telocyte research led to the identification of different subtypes suggestive for a heterogeneous telocyte population which can even coexist in the same location. As a consequence, the functions of TCs are still elusive and can be considered a versatile phenomenon that depends on a variety of conditions, including signal reception and transmission of information via extracellular vesicles or by direct intercellular contact.

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Inhibitory effects of methotrexate on spontaneous motility and Cajal-like type of tubal interstitial cells in rabbit oviduct

Cited by 6 publications

References 38 publications

Telocytes damage in endometriosis‐affected rat oviduct and potential impact on fertility

Telocytes damage in endometriosis‐affected rat oviduct and potential impact on fertility

Oviductal Telocytes in Patients with Uterine Myoma

Immunohistochemistry of Telocytes in the Uterus and Fallopian Tubes

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