Inhibitory Effects of Lutein on Endotoxin-Induced Uveitis in Lewis Rats

Jin, Xin; Ohgami, Kazuhiro; Shiratori, Kenji; Suzuki, Yukari; Hirano, Takeshi; Koyama, Yoshikazu; Yoshida, Kazuhiko; Ilieva, Iliyana; Iseki, Ken; Ohno, Shigeaki

doi:10.1167/iovs.05-1429

Cited by 109 publications

(120 citation statements)

References 23 publications

(23 reference statements)

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“…The dosing protocols were based on previous studies (15)(16)(17) . After seven days of acclimatization, the animals were randomly divided into four groups: Vehicle Control (vehicle only, n=6), vehicle pretreatment then LPS (Vehicle + LPS, n=6), lycopene pretreatment then LPS (Lycopene + LPS, n=7), and a positive control receiving de xamethasone pretreatment (DEX + LPS, n=7).…”

Section: Animals and Experimental Protocolmentioning

confidence: 99%

“…The identification of new anti-inflammatory compounds is thus required to reduce the complications induced by corticosteroids. Accordingly, numerous clinical and experimental studies have been conducted evaluating nonsteroidal treatment strategies for uveitis (15,(26)(27)(28) . Endotoxin-induced uveitis is a useful model for this purpose.…”

Section: Histopathology Scoresmentioning

confidence: 99%

“…Other carotenoids, such as lutein, fucoxanthin, and astaxanthin, also reduce inflammation in EIU (15,(26)(27)(28) . The anti-inflammatory effects of lutein on EIU were attributed to inhibition of NF-kB-dependent signaling (15) , while astaxanthin was suggested to inhibit inflammation via suppression of NO, PGE2, and TNF-α by directly blocking nitric oxide synthase (NOS) activity (26,28) .…”

Section: Histopathology Scoresmentioning

confidence: 99%

“…The anti-inflammatory effects of lutein on EIU were attributed to inhibition of NF-kB-dependent signaling (15) , while astaxanthin was suggested to inhibit inflammation via suppression of NO, PGE2, and TNF-α by directly blocking nitric oxide synthase (NOS) activity (26,28) . Similarly, fucoxanthin was reported to act by suppressing PGE-2 and NO production (27) .…”

Section: Histopathology Scoresmentioning

confidence: 99%

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Anti-inflammatory effect of lycopene on endotoxin-induced uveitis in rats

Göncü¹,

Oğuz²,

Sezen³

et al. 2016

Arquivos Brasileiros De Oftalmologia

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Section: Animals and Experimental Protocolmentioning

confidence: 99%

Section: Histopathology Scoresmentioning

confidence: 99%

Section: Histopathology Scoresmentioning

confidence: 99%

Section: Histopathology Scoresmentioning

confidence: 99%

See 2 more Smart Citations

Anti-inflammatory effect of lycopene on endotoxin-induced uveitis in rats

Göncü¹,

Oğuz²,

Sezen³

et al. 2016

Arquivos Brasileiros De Oftalmologia

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“…This study indicates that increasing plasma carotenoid concentration may reduce risk of premature death or recurrence of tumor in HNSCC patients. In the relation of NF-B and carotenoids, lutein suppresses the production of nitric oxide (NO) and the expression of tumor necrosis factor (TNF)-␣, interleukin-6, prostaglandin-E2, monocyte chemoattractant protein-1 (MCP-1), and macrophage inflammatory protein (MIP)-2 on endotoxin-induced uveitis in rats by inhibiting NF-B activation (35). ␤-Carotene inhibited H. pylori-induced increase in ROS level, the activation of mitogen-activated protein kinases (p38, the c-Jun NH2-terminal protein kinases, the extracellular signal-regulated kinases), NF-B, and AP-1 and the expression of iNOS and COX-2 in AGS cells (31).…”

Section: Effect Of Carotenoids On H2o2-induced Mrna Expression and Prmentioning

confidence: 99%

β-Carotene and Lutein Inhibit Hydrogen Peroxide-Induced Activation of NF-κB and IL-8 Expression in Gastric Epithelial AGS Cells

Kim

Seo

Kim

2011

J Nutr Sci Vitaminol

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Summary Reactive oxygen species (ROS) including hydrogen peroxide (H 2 O 2 ) are involved in the pathogenesis of gastric inflammation. Interleukin-8 (IL-8) is a potent mediator of the inflammatory response by activating and recruiting neutrophils to the site of infection. Oxidant-sensitive transcription factor NF-B regulates the expression of IL-8 in the immune and inflammatory events. Carotenoids (carotenes and oxygenated carotenoids) show antioxidant and anti-inflammatory activities. Low intake of ␤ -carotene leads to high risk of gastric cancer. Oxygenated carotenoid lutein inhibited NF-B activation in experimental uveitis. The present study aims to investigate whether ␤ -carotene and lutein inhibit H 2 O 2 -induced activation of NF-B and expression of IL-8 in gastric epithelial AGS cells. The cells were treated with carotenoids 2 h prior to the treatment of H 2 O 2 . mRNA expression was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and real time RT-PCR analyses. IL-8 level in the medium was determined by enzyme-linked immunosorbent assay. NF-B activation was assessed by electrophoretic mobility shift assay. ROS levels of the cells were detected by confocal microscopic analysis for fluorescent dichlorofluorescein. As a result, H 2 O 2 induced the activation of NF-B and expression of IL-8 in AGS cells time-dependently. ␤ -Carotene and lutein showed inhibitory effects on H 2 O 2 -induced increase in intracellular ROS levels, activation of NF-B, and IL-8 expression in AGS cells. In conclusion, supplementation of carotenoids such as ␤ -carotene and lutein may be beneficial for the treatment of oxidative stress-mediated gastric inflammation.

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