Inhibitory effects of (−)-loliolide on cellular senescence in human dermal fibroblasts

Yang, Haibin; Hwangbo, Kyoung; Zheng, Ming Shan; Cho, Jung Hee; Son, Jong‐Keun; Kim, Hwa Young; Baek, Suk Hwan; Choi, Hyung Chul; Park, So Young; Kim, Jae‐Ryong

doi:10.1007/s12272-014-0435-0

Cited by 28 publications

(17 citation statements)

References 19 publications

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“…In addition, we analyzed the nineteen different types of phytochemical components including of loliolide in the G. affine chloroform-soluble fraction having an anti-inflammatory action by GC-MS. Coniferyl alcohol is known as mediators of eugenol and isoeugenol biosynthesis [43], and loliolide is as having an inhibitory effect on cellular senescence in human dermal fibroblast [44]. In addition, the COX-2 inhibitory activity of vanillin and guaiacol is known [45].…”

Section: Discussionmentioning

confidence: 99%

The Anti-inflammatory Effect of Gnaphalium affine Through Inhibition of NF-κB and MAPK in Lipopolysaccharide-Stimulated RAW264.7 Cells and Analysis of Its Phytochemical Components

Seong

Hwang

Kim

2016

Cell Biochem Biophys

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Gnaphalium affine is an annual herbaceous plant that is used as a traditional medicine in some Latin American and Asian countries. However, systematic studies on its anti-inflammatory activity and signaling pathways have not yet been reported. In this study, we investigated the anti-inflammatory effect of G. affine methanol extract in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cells and fractioned the methanol extract into hexane, chloroform, ethyl acetate (EtOAc), butyl alcohol (BuOH), and distilled water (DW) by measuring the generation of nitric oxide (NO). G. affine inhibited the generation of NO and prostaglandin E2. The chloroform-soluble fraction most effectively inhibited LPS-stimulated NO production. We also examined the cytotoxicity of G. affine in three normal cell lines: RAW264.7, HEK293, and HaCaT. Cell viability assays showed that the methanol extract and chloroform-soluble fraction of G. affine had no cytotoxic effect on normal cell lines. The expression of pro-inflammatory mediators was also investigated. Western blotting and immunofluorescence showed that G. affine reduces the expression of iNOS, COX-2, and MAPKs, as well as activation of NF-κB in LPS-stimulated RAW264.7 cells. RT-PCR showed that G. affine also negatively regulates inflammatory cytokines at the gene expression level. Taken together, G. affine exerts its anti-inflammatory activity through inhibition of NO generation as a result of inhibiting NF-κB and MAPKs-related inflammatory signaling pathways. In addition, the result of GC-MS analysis revealed the presence of nineteen different types of constituents including guaiacol in the chloroform-soluble fraction of G. affine.

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Section: Discussionmentioning

confidence: 99%

The Anti-inflammatory Effect of Gnaphalium affine Through Inhibition of NF-κB and MAPK in Lipopolysaccharide-Stimulated RAW264.7 Cells and Analysis of Its Phytochemical Components

Seong

Hwang

Kim

2016

Cell Biochem Biophys

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“…Natural compounds derived from plants, including juglanin (59), quercetin-3- O -β-D-glucuronide (60), (−)-loliolide (61), and quercetagetin 3,4′-dimethyl ether (62) were identified as senomorphic candiates, when they decreased SAβG and p53 levels in senescent HDFs and HUVECs.…”

Section: Senomorphicsmentioning

confidence: 99%

Senotherapeutics: emerging strategy for healthy aging and age-related disease

2019

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Cellular senescence (CS) is one of hallmarks of aging and accumulation of senescent cells (SCs) with age contributes to tissue or organismal aging, as well as the pathophysiologies of diverse age-related diseases (ARDs). Genetic ablation of SCs in tissues lengthened health span and reduced the risk of age-related pathologies in a mouse model, suggesting a direct link between SCs, longevity, and ARDs. Therefore, senotherapeutics, medicines targeting SCs, might be an emerging strategy for the extension of health span, and prevention or treatment of ARDs. Senotherapeutics are classified as senolytics which kills SCs selectively; senomorphics which modulate functions and morphology of SCs to those of young cells, or delays the progression of young cells to SCs in tissues; and immune-system mediators of the clearance of SCs. Some senolytics and senomorphics have been proven to markedly prevent or treat ARDs in animal models. This review will present the current status of the development of senotherapeutics, in relation to aging itself and ARDs. Finally, future directions and opportunities for senotherapeutics use will discussed. This knowledge will provide information that can be used to develop novel senotherapeutics for health span and ARDs.

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“…(À)-Loliolide of P. aviculare exerted inhibitory activity against cellular senescence in human dermal fibroblasts (Yang et al, 2014a). Quercetin-3-O-b-D-glucuronide isolated from P. aviculare inhibits cellular senescence in human primary cells (Yang et al, 2014b).…”

Section: Anticancer Activitymentioning

confidence: 99%