Inhibitory Effects of Indigowoad Root Polysaccharides on Porcine Reproductive and Respiratory Syndrome Virus Replication <i>In Vitro</i>

Wei, Zhanyong; Xuebing, Wang; Zhang, Hongying; Yang, Chunhua; Wang, Yabin; Xu, D.; Chen, Hongying; Bao-an, Cui

doi:10.3851/imp1755

Cited by 23 publications

(15 citation statements)

References 26 publications

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“…Some polysaccharides isolated from plants have demonstrated significant inhibitory effects against animal viruses222728. We have proven that TPPPS, a polysaccharide extracted from P. massoniana pollen, inhibits ALV-J replication in a dose-dependent manner in vitro and that TPPPS pre-treatment in the first hour of virus infection indicates the strongest inhibition.…”

Section: Discussionmentioning

confidence: 93%

Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity

Wei

Liu

et al. 2017

Sci Rep

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Subgroup J avian leucosis virus (ALV-J) generally causes neoplastic diseases, immunosuppression and subsequently increases susceptibility to secondary infection in birds. The spread of ALV-J mainly depends on congenital infection and horizontal contact. Although ALV-J infection causes enormous losses yearly in the poultry industry worldwide, effective measures to control ALV-J remain lacking. In this study, we demonstrated that Taishan Pinus massoniana pollen polysaccharide (TPPPS), a natural polysaccharide extracted from Taishan Pinus massoniana pollen, can significantly inhibit ALV-J replication in vitro by blocking viral adsorption to host cells. Electron microscopy and blocking ELISA tests revealed that TPPPS possibly blocks viral adsorption to host cells by interacting with the glycoprotein 85 protein of ALV-J. Furthermore, we artificially established a congenitally ALV-J-infected chicken model to examine the anti-viral effects of TPPPS in vivo. TPPPS significantly inhibited viral shedding and viral loads in immune organs and largely eliminated the immunosuppression caused by congenital ALV-J infection. Additionally, pre-administration of TPPPS obviously reduced the size and delayed the occurrence of tumors induced by acute oncogenic ALV-J infection. This study revealed the prominent effects and feasible mechanisms of TPPPS in inhibiting ALV-J infection, thereby providing a novel prospect to control ALV-J spread.

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Section: Discussionmentioning

confidence: 93%

Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity

Wei

Liu

et al. 2017

Sci Rep

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“…One such pathogen is the porcine reproductive and respiratory syndrome virus (PRRSV), a major, devastating viral disease in pigs, for which vaccine strategies have failed to control outbreaks [6, 7], and only a few reports have described the use of medicinal compounds against it [8, 11, 24]. The present study aimed to evaluate the antiviral activity of C. dactylon against PRRSV in vitro .…”

Section: Resultsmentioning

confidence: 99%

In VitroVirucidal and Virustatic Properties of the Crude Extract ofCynodon dactylonagainst Porcine Reproductive and Respiratory Syndrome Virus

Pringproa

Khonghiran

Kunanoppadol

et al. 2014

Veterinary Medicine International

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The in vitro virustatic and virucidal tests of the crude extract of Cynodon dactylon against infection with porcine reproductive and respiratory syndrome virus (PRRSV), a cause of major devastating pig disease, were described. Crude extract of C. dactylon was prepared for cytotoxicity on tissue-culture cells that were used to measure virustatic and virucidal activities against PRRSV. Crude extract of C. dactylon at 0.78 mg/mL showed no cytotoxicity on the cell line, and at that concentration significantly inhibited replication of PRRSV as early as 24 hours post infection (hpi). C. dactylon also inactivated PRRSV as determined by immunoperoxidase monolayer assay (IPMA) compared to the control experiments. In summary, the present study may be among the earliest studies to describe virustatic and virucidal activities of C. dactylon crude extract against PRRSV in vitro. Extracts of C. dactylon may be useful for PRRSV control and prevention on pig farms.

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“…Therefore, successful supplementary strategies should include antiviral drugs and immune-modulation therapies (Benfield et al, 2002;Du et al, 2011;Han et al, 2009;Jiang et al, 2010;Karuppannan et al, 2012;Keirstead et al, 2008;Kreutz & Ackermann, 1996;Li et al, 2009;Opriessnig et al, 2011;Patel et al, 2008;te Velthuis et al, 2010;van der Meer et al, 2007;Wei et al, 2011;Yang et al, 2013;Zhuge et al, 2012). When evaluating the antiviral effect of tilmicosin against PRRSV, our data suggested that tilmicosin would be effective in reducing the PRRSV loads in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…These data indicate that reducing the PRRSV load in the serum may prevent pigs from developing clinical signs after PRRSV infection. Several studies have attempted to identify effective anti-PRRSV treatments in vitro and in vivo (Benfield, Chase, Moore, Wagner, & Zeman, 2002;Du, Yoo, Paradis, & Scherba, 2011;Han, Fan, Patel, & Zhang, 2009;Jiang, Fang, Luo, Xiao, & Chen, 2010;Karuppannan, Wu, Qiang, Chu, & Kwang, 2012;Keirstead, Lee, Yoo, Brooks, & Hayes, 2008;Kreutz & Ackermann, 1996;Li et al, 2009;Opriessnig et al, 2011;Patel et al, 2008;te Velthuis et al, 2010;van der Meer et al, 2007;Wei et al, 2011;Yang et al, 2013;Zhuge et al, 2012). However, there are no effective commercial drugs available to prevent PRRSV infection in pigs in vivo.…”

Section: Introductionmentioning

confidence: 99%

Tilmicosin Reduces PRRSV Loads in Pigs in vivo

Lin¹,

Yang²,

Lin³

et al. 2015

JAS

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Porcine respiratory and reproductive syndrome virus (PRRSV) is an important pathogen having a significant economic impact on the swine industry worldwide. Tilmicosin is a new semi-synthetic macrolide antibiotic developed from tylosin B. Tilmicosin can enter pulmonary alveolar macrophages (PAMs) and inhibit the replication of PRRSV in PAMs in vitro. This study was conducted to evaluate the impact of tilmicosin in controlling the replication of PRRSV in vivo. Forty and 635 weaned piglets were randomly chosen from PRRSV-contaminated farrow-to-finish herds in Taiwan and China, respectively. The piglets were equally divided into two groups and housed in the same pen but separated into individual spaces. Tilmicosin (Tilmovet ® 20% premix, 400 mg/kg) was administered after weaning for 21 days (treated group). The untreated group of piglets did not receive tilmicosin. Blood samples were collected at 4, 6, 8, 10 and 12 weeks of age to detect of the PRRSV load. At 8 and 10 weeks of age, the tilmicosin-treated piglets had a significantly lower PRRSV load than the untreated piglets (P < 0.05) in Taiwan. At 6, 8, 10, and 12 weeks of age, the tilmicosin-treated piglets had a significantly lower PRRSV load than the untreated piglets (P < 0.05) in China. These data indicates that animals treated with tilmicosin exhibited not only reduced PRRSV loads but also improved average daily weight gain during the study period.

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Inhibitory Effects of Indigowoad Root Polysaccharides on Porcine Reproductive and Respiratory Syndrome Virus Replication In Vitro

Cited by 23 publications

References 26 publications

Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity

Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity

In VitroVirucidal and Virustatic Properties of the Crude Extract ofCynodon dactylonagainst Porcine Reproductive and Respiratory Syndrome Virus

Tilmicosin Reduces PRRSV Loads in Pigs in vivo

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