Inhibitory Effects of <i>Terminalia catappa</i> on UVB‐Induced Photodamage in Fibroblast Cell Line

Wen, Kuo‐Ching; Shih, I-Chen; Hu, Jhe-Cyuan; Liao, Sue-Tsai; Su, Tsung-Wei; Chiang, Hsiu-Mei

doi:10.1155/2011/904532

Cited by 32 publications

(39 citation statements)

References 49 publications

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“…The cells were treated with non-cytotoxic concentrations of the test samples for 2 to 24 hours. After treatment with the test samples, the cells were subjected to the lethal dose of UV-B irradiation (200 mJ/cm 2 ) that can lead to approximately 50% cytotoxicity (302 nm, CL-1000 M, UVP, USA) [33]. The percent cell viability was assessed using the following Equation 2: % Cell viability = (X*100)/R…”

Section: Anti-wrinkle Effects Of the Test Formulation On Hff-1 Cells mentioning

confidence: 99%

The Use of Consciousness Energy Healing Based Herbomineral Formulation for Skin Anti-Aging Strategies

Parulkar¹

2017

JFNS

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Abstract:The present study was undertaken to evaluate the effect of a Consciousness Energy Healing (The Trivedi Effect ® ) Treatment based test formulation and medium (DMEM) against skin health parameters using HFF-1, HaCaT, and B16-F10 cells. The test formulation and DMEM were divided into two parts. One part of the test formulation and one part of the DMEM received the Consciousness Energy Healing Treatment by Vaibhav Rajan Parulkar and were defined as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test samples. Cell viability using MTT assay showed more than 75% cells were viable in all the tested concentrations in three cells, indicating that the test formulation was safe and nontoxic. The percent cell proliferation by BrdU assay was significantly increased by 434.14%, 244.77%, and 268.53% in the UT-DMEM + BT-Test Formulation, BT-DMEM + UT-Test Formulation, and BT-DMEM + BT-Test Formulation groups, respectively at 8.75 µg/mL in relation to the UT-DMEM + UT-Test Formulation group. Elastin was significantly (p≤0.001) increased 93.52% and 75.81% in the UT-DMEM + BT-Test Formulation and BT-DMEM + UT-Test Formulation groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test Formulation group. Hyaluronic acid was significantly increased by 100.07% (p≤0.05), 41.60%, and 50.10% in the UT-DMEM + BT-Test Formulation, BT-DMEM + UT-Test Formulation, and BT-DMEM + BT-Test Formulation groups, respectively at 0.625 µg/mL in relation to the UT-DMEM + UTTest Formulation group. Melanin was decreased by 10.09% and 6.51% in the UT-DMEM + BT-Test Formulation and BT-DMEM + UT-Test Formulation groups, respectively at 0.125 µg/mL compared to the UT-DMEM + UT-Test Formulation group. Protection of skin cells after UV-B exposure data displayed that the cell viability was increased by 7.22%, 5.75%, and 8.15% in the BT-DMEM + BT-Test Formulation at 0.625%, 1.25%, and 2.5% µg/mL, respectively compared to the UT-DMEM + UT-Test Formulation group. Wound healing data exhibited significant wound closure and cell migration activities in the HFF-1 cells compared to the UT-DMEM + UT-Test formulation group. Overall, the data suggests that the Biofield Energy Treated DMEM and test formulation demonstrated better skin protection action compared to the untreated DMEM and test formulation. Therefore, the Biofield Energy Treated test herbomineral formulation could be useful for the development of effective cosmetic products for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc.

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Section: Anti-wrinkle Effects Of the Test Formulation On Hff-1 Cells mentioning

confidence: 99%

The Use of Consciousness Energy Healing Based Herbomineral Formulation for Skin Anti-Aging Strategies

Parulkar¹

2017

JFNS

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“…The cells were treated with non-cytotoxic concentrations of test items for 2 to 24 hours. After treatment with test items, the cells were subjected to the lethal dose of UV-B irradiation (200 mJ/cm 2 ) that can lead to approximately 50% cytotoxicity (302 nm, CL-1000 M, UVP, USA) [33]. The percent cell viability was assessed using following Equation 2:…”

Section: Anti-wrinkle Effects Of the Test Formulation On Hff-1 Cells mentioning

confidence: 99%

Skin Protective Activity of Consciousness Energy Healing Treatment Based Herbomineral Formulation

Smith¹

2017

JFNS

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Abstract:The current study was attempted to evaluate the impact of the Consciousness Energy Healing (The Trivedi Effect ® ) Treatment based herbomineral test formulation and cell medium (DMEM) against skin health. The test formulation and DMEM were divided into two parts. One of each part was received the Consciousness Energy Healing Treatment by Dennille Mellesia Smith and was termed as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test items. MTT showed >78% viable cells, indicating that the test formulation was safe and nontoxic in all the tested concentrations in three cell lines. The percent cell proliferation by BrdU assay was significantly increased by 238.30%, 192.06%, and 43.96% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL with respect to the UT-DMEM + UT-Test formulation group. The level of collagen was significantly increased by 55.55%, 32.65%, and 52.48% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 1.25 µg/mL compared to the untreated group. Elastin was significantly (p≤0.001) increased by 6.30%, 105.04%, and 29.41% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. Hyaluronic acid was increased by 4.78%, 29.71%, and 58.29% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.63 µg/mL compared to the UT-DMEM + UT-Test Formulation group. The level of melanin was reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation, respectively at 0.13 µg/mL compared to the untreated group. Skin protection against UV-B data displayed that cell proliferation was increased by 17.88%, 20.14%, and 9.89% in the BT-DMEM + BT-Test formulation at 0.63, 1.25, and 2.5 µg/mL, respectively compared to the untreated group. Wound healing activity exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Overall, result suggests that the Biofield Energy Treated DMEM and test formulation exhibited better responses compared to the untreated medium and test formulation. Therefore, the Biofield Energy Treated herbomineral formulation could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc.

show abstract

“…The cells were treated with non-cytotoxic concentrations of the test samples for 2 to 24 hours. After treatment with the test samples, the cells were subjected to the lethal dose of UV-B irradiation (200 mJ/cm 2 ) that can lead to approximately 50% cytotoxicity (302 nm, CL-1000 M, UVP, USA) [41]. The percent cell viability was assessed using formula (2): % Cell viability = (X*100)/R…”

Section: Anti-wrinkle Effects Of the Test Formulation On Hff-1 Cells mentioning

confidence: 99%

Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation

Holmlund¹,

Trivedi²,

Branton³

et al. 2017

EJPM

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Abstract:The aim of the present study was undertaken to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect ® ) Treatment based test formulation on skin health. The test formulation and DMEM were separated into two parts. One part of each received the Consciousness Energy Healing Treatment by Shirley Theresa Holmlund and were termed as the Biofield Energy Treated samples, while the other parts were considered as the untreated test samples. MTT assay showed more than 70% cells were viable in all the tested concentrations in three cell lines and that the test formulation was safe and nontoxic. The percent cell proliferation using BrdU assay was significantly increased by 343.57%, 100.29%, and 132.6% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. The collagen synthesis was significantly increased by 5.94% and 10.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Elastin level was significantly (p≤0.001) increased by 15.95%, 58.31%, and 47.18% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the untreated group. The level of hyaluronic acid was significantly increased by 15.13% and 68.56% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. The level of melanin was significantly reduced by 17.06%, 6.94%, and 13.07% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.063 µg/mL compared to untreated group. The effect of skin protection upon exposure with UV-B rays displayed some alteration of the percent cell viability in all the tested groups. The wound healing activity by scratch assay exhibited significant wound closure and cell migration in the test formulation and DMEM in HFF-1 cells compared to the untreated group. Altogether, the data suggests that the Consciousness Energy Healing Treated DMEM and test formulation exhibited better skin protective responses compared to the untreated DMEM and test formulation group with respect to the skin health parameters. Therefore, the Biofield Energy Treated test formulation could be developed as a potential cosmetic product against various skin problems viz.

show abstract

Inhibitory Effects of Terminalia catappa on UVB‐Induced Photodamage in Fibroblast Cell Line

Cited by 32 publications

References 49 publications

The Use of Consciousness Energy Healing Based Herbomineral Formulation for Skin Anti-Aging Strategies

The Use of Consciousness Energy Healing Based Herbomineral Formulation for Skin Anti-Aging Strategies

Skin Protective Activity of Consciousness Energy Healing Treatment Based Herbomineral Formulation

Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation

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