Inhibitory Effects of Brassica rapa L. and Raphanus sativus L. on Adipogenesis and Lipogenesis through PI3K/AKT Pathway Regulation in C3H10T1/2 Cells

Abstract

Radish (Raphanus sativus L.) is a representative root vegetable widely cultivated in Asia and known to have various physiological effects, such as gastric protective, anti-inflammatory, antioxidant, and hemostasis improving effects, and to ameliorate digestive disorders. However, studies on the antiobesity effects of white and red radish are inadequate. Therefore, in this study, the anti-adipogenic and anti-lipogenic effects of Ganghwa (RG) and Jeju (RJ) radishes were investigated in C3H10T1/2 cells. Lipid accumulation inhibition by Oil Red O staining and TG contents confirmed that at 1,000 μg/mL RG and RJ significantly inhibited lipid accumulation and dose-dependently reduced the mRNA and protein levels of major adipogenesis and lipogenesis-related factors. In addition, inhibition of the expression of genes in the PI3K/Akt pathway by RG or RJ markedly increased leptin and adiponectin levels. These results indicate radishes are potential functional materials that inhibit adipocyte differentiation and lipid accumulation.