Inhibitory effects of free propionic and butyric acids on the activities of hydrogenotrophic methanogens in mesophilic mixed culture fermentation

Zhang, Wei; Zhang, Fang; Li, Yongxin; Zeng, Raymond J.

doi:10.1016/j.biortech.2018.10.076

Cited by 16 publications

(8 citation statements)

References 32 publications

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“…Butyric acid reaches C50% under a 0.73 g L –1 (1.3 g COD L –1 ) concentration. Zhang et al 32 . explain a similar behavior of the CH 4 production curves from butyric acid (Fig.…”

Section: Resultssupporting

confidence: 61%

“…3). Zhang et al 32 . worked with mesophilic batch reactors to determine the 50% (C50%) CH 4 inhibition with butyric acid as substrate.…”

Section: Resultsmentioning

confidence: 99%

“…3). Zhang et al 32 worked with mesophilic batch reactors to determine the 50% (C50%) CH 4 inhibition with butyric acid as substrate. Butyric acid reaches C50% under a 0.73 g L -1 (1.3 g COD L -1 ) concentration.…”

Section: >97%mentioning

confidence: 99%

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Kinetic analysis of the methanization of the byproducts from OFMSW fermentation

Jojoa-Unigarro

González‐Martínez

2021

J of Chemical Tech & Biotech

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BACKGROUND During anaerobic digestion of wastes, volatile fatty acid (VFA) production has been studied, but little attention has been paid to alcohols and lactic acid. Thermodynamically, lactic acid and alcohols are better substrates to produce methane than other VFA. This research identified the metabolites produced during the fermentation of the organic fraction of municipal solid waste and studied their methanization in a second stage. Analysis of the methane production curves is provided and explained according to Gibbʼs free energy of acetogenesis and methanogenesis. Based on the Michaelis–Menten model, a kinetic analysis is presented. RESULTS The specific methane production of the identified acids and alcohols are acetic acid, 343 NL/kgCOD; ethanol, 296; methanol, 181; butyric acid, 108; lactic acid, 64. Lactic and butyric acids present a long adaptation phase followed by a fast and short methane production. Acetic acid and ethanol finished the reaction in less than 16 h. The highest methane production rates (Vmax) were for butyric acid, methanol, and ethanol with 2136, 1934, and 1928 NmL L–1, respectively. The best affinity (lowest Km) values were for acetic and lactic acids and ethanol with 1.2, 1.9, and 2.0 gCOD L–1. CONCLUSION Together, acetic acid and ethanol represent 92% of all fermentation products. No endogenous methane production was observed during the methanization from acetic acid and ethanol. The highest specific methane production belongs to acetic acid (98% of the theoretical) and the lowest corresponds to lactic acid. Except for propionic acid, the methanization of the selected substrates follows the Michaelis and Menten model. © 2021 Society of Chemical Industry (SCI).

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“…Butyric acid reaches C50% under a 0.73 g L –1 (1.3 g COD L –1 ) concentration. Zhang et al 32 . explain a similar behavior of the CH 4 production curves from butyric acid (Fig.…”

Section: Resultssupporting

confidence: 61%

“…3). Zhang et al 32 . worked with mesophilic batch reactors to determine the 50% (C50%) CH 4 inhibition with butyric acid as substrate.…”

Section: Resultsmentioning

confidence: 99%

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Kinetic analysis of the methanization of the byproducts from OFMSW fermentation

Jojoa-Unigarro

González‐Martínez

2021

J of Chemical Tech & Biotech

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“…They tested the specific methanogenic activity against pH, acid concentration and the concentration of free acids and concluded that the free acids are the key factor in inhibiting methanogenesis. The results obtained by this study showed that the concentration of free acetic acids does not surpass the thresholds for total inhibition mentioned by Zhang et al, but partial inhibition is not eliminated [61,62]. Further studies are needed to verify to what extent the storage temperature and the cumulative concentration of the different free acids could potentialize the inhibitory effect on methanogenic activity.…”

Section: Dairy Manurementioning

confidence: 54%

“…Another explanation for the observed low methane emissions is presented by Zhang et al, who showed that in a mesophilic mixed culture, the inhibition of hydrogenotrophic methanogens is caused by the concentration of free acetic, propionic and butyric acids [61,62]. They tested the specific methanogenic activity against pH, acid concentration and the concentration of free acids and concluded that the free acids are the key factor in inhibiting methanogenesis.…”

Section: Dairy Manurementioning

confidence: 99%

Methane Emissions from Livestock Slurry: Effects of Storage Temperature and Changes in Chemical Composition

Hilgert

Amon

et al. 2022

Sustainability

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Livestock production contributes to releasing methane into the atmosphere. Liquid manure management offers significant opportunities to reduce these emissions. A better understanding of the factors controlling methane emissions from manure is necessary to select effective mitigation strategies. Our study aimed to identify the influence of storage temperature and the associated change in chemical composition on methane emissions from dairy and fattening pig manure. Storage temperature affects microbial activity and induces changes in chemical composition that are key influences in methane emissions. Dairy and fattening pig manure samples were stored at five different temperatures (5–25 °C) for 90 days in a laboratory-scale experiment to measure the methane production. The chemical composition of the slurry samples was analyzed, and the biochemical methane potential (BMP) tests were performed before and after storage. For pig manure stored at 25 °C and 20 °C, methane emissions accounted for 69.3% and 50.3% of the BMP, respectively. Maximum methane emissions for dairy slurry were observed at 25 °C but remained at a low level. Analyses of the accumulation of volatile fatty acids (VFAs) during storage are presented in few studies, this work revealed a potential inhibition of methane production, where the accumulation of VFAs was most elevated in samples stored at 20 °C and 25 °C. This partly counteracted the increase in methane emissions expected from the higher temperatures. The degree of VFA and dissociated fatty acids accumulation in dairy cattle slurry should be assessed for more accurate estimations of methane emissions from slurry stores.

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Pig manure degradation and carbon emission: Measuring and modeling combined aerobic–anaerobic transformations

Dalby,

Hafner,

Ambrose

et al. 2024

J of Env Quality

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Greenhouse gas emissions from liquid livestock manure storage significantly contribute to global warming. Accurate farm‐scale models are essential for predicting these emissions and evaluating manure management strategies, but they rely on multiple parameters describing carbon loss dynamics. Surface respiration may significantly influence carbon loss and methane emission, yet it is not explicitly included in current models. We conducted experiments to measure pig manure surface respiration rate and its effect on organic matter degradation and methane and carbon dioxide emissions. Manure was incubated for 283 days at 10°C or 20°C under aerobic or anaerobic conditions, while measuring methane and carbon dioxide emission. This was followed by anaerobic digestion at 38°C. Surface respiration reduced the organic matter content, and the effect was temperature dependent. Methane emission was not affected by surface respiration, suggesting that substrate availability was not rate‐limiting for methanogenesis. Surface respiration rates were 18.1 ± 3.5 g CO2 m−2 day−1 at 10°C and 37.1 ± 13.1 g CO2 m−2 day−1 at 20°C (mean ± standard deviation) and were consistent with microsensor measurements of oxygen consumption in different manure surfaces. Based on these results, temperature‐ and surface area‐dependent respiration was incorporated in the existing anaerobic biodegradation model (ABM). Simulations showed that surface respiration accounts for 29% of carbon losses in a typical pig house and 8% for outdoor storage. Developing and refining algorithms for diverse carbon transformations, such as surface respiration, is crucial for evaluating the potential for methane emission and identification of variables that control emissions at the farm scale.

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Inhibitory effects of free propionic and butyric acids on the activities of hydrogenotrophic methanogens in mesophilic mixed culture fermentation

Cited by 16 publications

References 32 publications

Kinetic analysis of the methanization of the byproducts from OFMSW fermentation

Kinetic analysis of the methanization of the byproducts from OFMSW fermentation

Methane Emissions from Livestock Slurry: Effects of Storage Temperature and Changes in Chemical Composition

Pig manure degradation and carbon emission: Measuring and modeling combined aerobic–anaerobic transformations

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