Inhibitory effects of essential oil from rhizomes of<i>Ligusticum chuanxiong</i>on hypertrophic scarring in the rabbit ear model

Wu, Jianguo; Wei, Yan-Jie; Ran, Xia; Zhang, Hong; Ni, Hua; Qin, Lu‐Ping

doi:10.3109/13880209.2010.542761

Cited by 19 publications

(17 citation statements)

References 22 publications

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“…The scar elevation index was measured as described in previous publications (Wu et al, 2011). For immunohistochemical staining, the sections were detected with primary antibodies against a-SMA (Sigma-Aldrich, St Louis, MO), proliferating cell nuclear antigen (Epitomics, Burlingame, CA), CD4, and MOMA2 (monocytes/ macrophages marker) (Abcam, Cambridge, MA) overnight at 4 1C.…”

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

Xiamenmycin Attenuates Hypertrophic Scars by Suppressing Local Inflammation and the Effects of Mechanical Stress

Liu

Fan

et al. 2013

Journal of Investigative Dermatology

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Hypertrophic scarring is a common disease affecting millions of people around the world, but there are currently no satisfactory drugs to treat the disease. Exaggerated inflammation and mechanical stress have been shown to be two main mechanisms of excessive fibrotic diseases. Here we found that a benzopyran natural product, xiamenmycin, could significantly attenuate hypertrophic scar formation in a mechanical stretch-induced mouse model. The compound suppressed local inflammation by reducing CD4+ lymphocyte and monocyte/macrophage retention in fibrotic foci and blocked fibroblast adhesion with monocytes. Both in vivo and in vitro studies found that the compound inhibited the mechanical stress-induced profibrotic effects by suppressing proliferation, activation, fibroblast contraction, and inactivating FAK, p38, and Rho guanosine triphosphatase signaling. Taken together, the compound could simultaneously suppress both the inflammatory and mechanical stress responses, which are the two pivotal pathological processes in hypertrophic scar formation, thus suggesting that xiamenmycin can serve as a potential agent for treating hypertrophic scar formation and other excessive fibrotic diseases.

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Section: Histology and Immunohistochemistrymentioning

confidence: 99%

Xiamenmycin Attenuates Hypertrophic Scars by Suppressing Local Inflammation and the Effects of Mechanical Stress

Liu

Fan

et al. 2013

Journal of Investigative Dermatology

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“…Our previous investigations indicated that EO extracted from the plant markedly suppressed the viability of human hypertrophic scar fibroblasts, elicited cell apoptosis in vitro (Wu et al, 2011a), and inhibited the formation and development of hypertrophic scars in the rabbit ear model (Wu et al, 2011b;Zhang et al, 2012), suggesting its protective effects against hypertrophic scars. However, as far as we are aware no investigations have been carried out to evaluate safety of essential oil extracted from rhizomes of Ligusticum chuanxiong, which is crucial for its clinical applications.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the chemical composition, acute toxicity and skin sensitivity of essential oil from rhizomes of Ligusticum chuanxiong

Zhang

Han

et al. 2012

Journal of Ethnopharmacology

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“…After all, drugs are more commonly used to treat formed hypertrophic scars clinically and the good preventive effect of a drug does not mean its favorable therapeutic action. Therefore we further investigated the therapeutic effect of the essetial oil (EO) from Ligusticum chuanxiong on formed hypertrophic scars in the rabbit ear model on the basis of our previous preliminary investigations [18], [19] for a show of its clinical value.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous preliminary investigations indicated that EO extracted from the plant markedly suppressed the viability of human hypertrophic scar fibroblasts (HSFs), elicited cell apoptosis in vitro [18], and inhibited hypertrophic scar formation in the rabbit ear model [19], suggesting its preventive effects on hypertrophic scarring. Nevertheless the good preventive effect of a drug does not mean its favorable therapeutic action.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effects of Liposome-Enveloped Ligusticum chuanxiong Essential Oil on Hypertrophic Scars in the Rabbit Ear Model

Zhang

Ran

et al. 2012

PLoS ONE

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Hypertrophic scarring, a common proliferative disorder of dermal fibroblasts, results from an overproduction of fibroblasts and excessive deposition of collagen. Although treatment with surgical excision or steroid hormones can modify the symptoms, numerous treatment-related complications have been described. In view of this, we investigated the therapeutic effects of essential oil (EO) from rhizomes of Ligusticum chuanxiong Hort. (Umbelliferae) on formed hypertrophic scars in a rabbit ear model. EO was prepared as a liposomal formulation (liposome-enveloped essential oil, LEO) and a rabbit ear model with hypertrophic scars was established. LEO (2.5, 5, and 10%) was applied once daily to the scars for 28 days. On postoperative day 56, the scar tissue was excised for masson's trichrome staining, detection of fibroblast apoptosis, assays of the levels of collagens I and III, and analysis of the mRNA expression of matrix metalloproteinase-1 (MMP-1), caspase-3 and -9, and transforming growth factor beta 1 (TGF-β1). In addition, the scar elevation index (SEI) was also determined. As a result, LEO treatment significantly alleviated formed hypertrophic scars on rabbit ears. The levels of TGF-β1, MMP-1, collagen I, and collagen III were evidently decreased, and caspase -3 and -9 levels and apoptosis cells were markedly increased in the scar tissue. SEI was also significantly reduced. Histological findings exhibited significant amelioration of the collagen tissue. These results suggest that LEO possesses the favorable therapeutic effects on formed hypertrophic scars in the rabbit ear model and may be an effective cure for human hypertrophic scars.

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Inhibitory effects of essential oil from rhizomes ofLigusticum chuanxiongon hypertrophic scarring in the rabbit ear model

Cited by 19 publications

References 22 publications

Xiamenmycin Attenuates Hypertrophic Scars by Suppressing Local Inflammation and the Effects of Mechanical Stress

Xiamenmycin Attenuates Hypertrophic Scars by Suppressing Local Inflammation and the Effects of Mechanical Stress

Analysis of the chemical composition, acute toxicity and skin sensitivity of essential oil from rhizomes of Ligusticum chuanxiong

Therapeutic Effects of Liposome-Enveloped Ligusticum chuanxiong Essential Oil on Hypertrophic Scars in the Rabbit Ear Model

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