Inhibitory Effects of Donkey Hide Gelatin on DNCB-Induced Atopic Dermatitis in NC/Nga Mice

Abstract

The increase of atopic dermatitis has led to higher socio-economic cost and raised a need for alternative medicine as novel therapeutic agents. In this study, we aimed to evaluate the inhibitory effects of Donkey Hide Gelatin (DHG) water extract on DNCB-induced atopic dermatitis in NC/Nga mice and on tumor necrosis factor (TNF)-α/interferon (IFN)-γ-treated keratinocytes and to investigate its underlying molecular mechanisms. NC/Nga mice were induced by DNCB, administered Dexamethasone (3 mg/kg) or DHG water extracts (100–400 mg/kg) for 3 weeks. The skin symptom score, serum IgE and immune cells were measured, the ALN, spleen and dorsal skin tissue were extracted for FACS, quantitative real-time PCR and histology analysis. In vitro, HaCaT cells were induced by TNF-α/IFN-γ, the levels of pro-inflammatory cytokines and chemokines and its underlying mechanism were measured by ELISA and Western blot. As a result, DHG groups showed a significant decrease in the skin symptom score and the immune cell absolute number. It also showed a marked reduction of allergic and the levels of neutrophils and eosinophils in histology analysis. In TNF-α/IFN-γ induced HaCaT cells, DHG showed inhibition effects on IL-6, IL-8, TARC and RANTES, it also downregulated the expression of ICAM-1 and COX-2, up-regulated the expression of Filaggrin. Furthermore, DHG suppressed the activation of NF-κB and mitogen-activated protein kinases (MAPK) signaling pathway induced by TNF-α/IFN-γ. Taken together, DHG maybe a potential therapeutic agent or supplement for skin inflammatory disease such as atopic dermatitis.

Identification and Molecular Mechanism of Novel Immunomodulatory Peptides from Gelatin Hydrolysates: Molecular Docking, Dynamic Simulation, and Cell Experiments

“…Food-derived immunomodulatory peptides regulated the innate immune system by binding to specific receptors on the surface of target cells. , Immunomodulatory peptides can stimulate the release of cytokines to enhance immunity or reduce the LPS-induced production of inflammatory cytokines to achieve the anti-inflammatory effect . Immunomodulatory peptides have been isolated from various foods and identified using in vitro enzymolysis via trypsin and pepsin. − In recent years, donkey-hide gelatin has attracted significant attention from researchers throughout the world because of its various activities, such as replenishing blood, anti-photoaging, immunity-enhancing, and anti-inflammation. , Donkey-hide gelatin is prepared from the skin of Equus asinus L, and it has been reported to be rich in protein (60–80% protein by weight) − Consequently, donkey-hide gelatin is considered to be an excellent source of immunomodulatory peptides. In particular, low-molecular-weight peptides (below 1000 Da) derived from donkey-hide gelatin have been demonstrated to improve the humoral immunity and cell-mediated immunity of mice…”

“…12−14 In recent years, donkey-hide gelatin has attracted significant attention from researchers throughout the world because of its various activities, such as replenishing blood, anti-photoaging, immunity-enhancing, and anti-inflammation. 15,16 Donkey-hide gelatin is prepared from the skin of Equus asinus L, and it has been reported to be rich in protein (60−80% protein by weight) 17−19 to be an excellent source of immunomodulatory peptides. In particular, low-molecular-weight peptides (below 1000 Da) derived from donkey-hide gelatin have been demonstrated to improve the humoral immunity and cell-mediated immunity of mice.…”

Characterization of a novel galectin in Sarcoptes scabiei and its role in regulating macrophage functions

“…Currently, AD models are often used in fundamental research on allergic dermatitis. Many studies have employed 1-chloro-2,4-dinitroenzene (DNCB) to induce a persistent clinical AD model (Lee et al, 2022 ). Tacrolimus is a macrolide immunosuppressant that is widely used to treat AD.…”

The Influence of Gamma Radiation on Different Gelatin Nanofibers and Gelatins

“…The development of new wound care biomaterials is relevant for the high incidence of skin injuries and hard-to-heal wounds with morbidity and mortality risks [1]. The polyamphiphilic properties of gelatin enable its structuring in versatile materials with advanced functionalities for food [2][3][4][5], cosmetic [6][7][8][9][10], pharmacy [11][12][13], and medical applications [14][15][16][17].…”