Inhibitory effects of delphinidin and luteolin on genotoxicity induced by K2B3O3F4OH) in human lymphocytes in vitro

Abstract

The genotoxicity of halogenated boroxine [K2(B3O3F4OH)], a novel compound with the potential for prevention and/or treatment of various skin changes, has been confirmed in human lymphocytes. The potential of luteolin and delphinidin in inhibition of the genotoxic and cytotoxic effects of halogenated boroxine in vitro was analyzed applying the chromosome aberration analysis and the cytokinesis-block micronucleus cytome assay in human lymphocyte cultures. The in vitro treatments included addition of boroxine and flavonoids independently, and combined treatments of boroxine with luteolin or delphinidin. In the concentration of 50 µM, luteolin significantly decreased the frequency of micronuclei and nuclear buds. Delphinidin suppressed the occurrence of aberrant cells in the presence of the halogenated boroxine, but also affected their induction in respective delphinidin treatment. Detection of endoreduplications in combined treatments indicated that these flavonoids are potential inhibitors of cell cycle or topoisomerase II activity. The obtained results have confirmed antigenotoxic activity of selected bioflavonoids in vitro. The side effects of potential therapeutic applications of halogenated boroxine may be inhibited in the presence of bioflavonoids in appropriate dosage.

Effects of Curcumin and Luteolin on Viability and Cell Death Induction in NFS-60 Cell Line

“…Research of Chen et al (2018) in THP-1 cell line show that increase in luteolin concentration results in decrease of cell viability while decrease of cell viability is not registered for normal monoclonal peripheral blood cells. Our previous research have also shown that luteolin in concentration of 50 µM protects and inhibits genotoxic damage in normal human lymphocytes culture (Hadžić et al, 2015). In the conditions of our experiment, curcumin and luteolin show different effects in the concentration of 10 μM, with curcumin decreasing and luteolin increasing NFS-60 cell culture viability, while in the lowest concentration (5 μM), both tested agents increase and in the highest concentration (20 μM) decrease cell culture viability.…”

Inhibition of Horseradish Peroxidase Activity by Boroxine Derivative, Dipotassium-trioxohydroxytetrafluorotriborate K2[B3O3F4OH]

“…First published effects of its bioactivity revealed a potential for inhibition of lymphocytes proliferation and cell growth of basal cell carcinoma culture as well as certain clastogenic potential [5]. Recently it has been confirmed that selected flavonoids may inhibit damage of genetic material in human lymphocytes induced by K 2 (B 3 O 3 F 4 OH) [6]. Previous findings of antitumour activity of K 2 (B 3 O 3 F 4 OH) in vitro and in vivo on 4T1 mammary carcinoma, B16F10 melanoma, and squamous cell carcinoma SCVII revealed inhibitory effects on cell proliferation in concentration of 1 mM, while concentrations less than 0.1 mM do not significantly affect cell growth in vitro.…”

Moderate Toxicity of Potential Boron-containing Therapeutic, Dipotassium-trioxohydroxytetrafl uorotriborate -K2(B3O3F4OH) in Rats and Mice

“…It structurally resembles the BRAF (B-Raf Serine/ Threonine Kinase enzyme) and MEK (mitogen-activated protein kinase kinase enzyme) inhibitors often used for treatment of metastatic melanoma caused by mutations in BRAF or MEK genes. Since the patenting of HB as a prevention and therapeutic agent for various skin changes (Galic, 2012;2013), numerous in vitro analyses were conducted to investigate its biological activities (Haveric et al, 2011;Hadzic et al, 2015;Haveric et al, 2016;Hadzic et al, 2019). So far, anti-tumour activity of HB, particularly towards mammary carcinoma (4T1), melanoma (B16F10) and squamous cell carcinoma (SCCVII) in vitro has been proven (Ivankovic et al, 2015).…”