Inhibitory effects of cranberry polyphenol and volatile extracts on nitric oxide production in LPS activated RAW 264.7 macrophages

Moore, Katie L.; Howard, Luke R.; Brownmiller, Cindi; Gu, Inah; Lee, Sun‐Ok; Mauromoustakos, Andy

doi:10.1039/c9fo01500k

Cited by 27 publications

(27 citation statements)

References 37 publications

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“…Cranberry is well known for its inhibitory action on NO production. Moore et al suggested that cranberry polyphenol and volatile extracts induced a 46-fold decrease in NO production in LPS-activated RAW 264.7 macrophages [38]. In the present study, SHE caused 38% inhibition of NO production induced by LPS in RAW 264.7 cells.…”

Section: Discussionsupporting

confidence: 61%

Anti-Inflammatory and Antioxidant Effects of Soroseris hirsuta Extract by Regulating iNOS/NF-κB and NRF2/HO-1 Pathways in Murine Macrophage RAW 264.7 Cells

Lee

et al. 2021

Applied Sciences

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Until now, the physiological effects of Soroseris hirsuta were primarily unknown. Here we have evaluated the anti-inflammatory and antioxidant effects of Soroseris hirsuta extract (SHE) on lipopolysaccharide (LPS)-activated murine macrophages RAW 264.7 cells. SHE inhibited nitric oxide expression and inducible nitric oxide synthase expression in RAW 264.7 cells treated with LPS. Moreover, SHE suppressed LPS-induced phosphorylation of IκB kinase, inhibitor of kappa B, p65, p38, and c-JUN N-terminal kinase. Western blot and immunofluorescence analyses showed that SHE suppressed p65 nuclear translocation induced by LPS. Furthermore, SHE inhibited the reactive oxygen species in LPS-treated RAW 264.7 cells. SHE significantly increased heme oxygenase-1 expression and the nuclear translocation of nuclear factor erythroid 2-related factor 2. SHE suppressed LPS-induced interleukin-1β mRNA expression in RAW 264.7 cells. Thus, SHE is a promising nutraceutical as it displays anti-inflammatory and antioxidant properties.

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Section: Discussionsupporting

confidence: 61%

Anti-Inflammatory and Antioxidant Effects of Soroseris hirsuta Extract by Regulating iNOS/NF-κB and NRF2/HO-1 Pathways in Murine Macrophage RAW 264.7 Cells

Lee

et al. 2021

Applied Sciences

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“…Strawberry extract (100 μg/mL) also a showed significant decrease in LPS-stimulated NO production, compared to the control without LPS ( p < 0.05) [ 92 ]. In a recent study, Moore et al [ 28 ] compared the NO-inhibitory effect of cranberry polyphenols and volatiles. Both cranberry polyphenol (317.8 and 635.7 μg/g) and volatile (1.8 μg/g) extracts significantly suppressed LPS-induced NO production ( p < 0.05).…”

Section: Discussionmentioning

confidence: 99%

“…Berry volatile extract (200 mL) was collected in an ice bath incubated flask and stored at −20 °C until use. Solid phase micro-extraction (SPME) of volatiles was performed using a 85 μm, CAR/PDMS, Stableflex, 24 Ga, Manual Supelco (Bellefonte, PA, USA) SPME fiber as previously described [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

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Berry Phenolic and Volatile Extracts Inhibit Pro-Inflammatory Cytokine Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κB Signaling Pathway

Brownmiller

Stebbins

et al. 2020

Antioxidants

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Berries are a rich source of phytochemicals, especially phenolics well known for protective activity against many chronic diseases. Berries also contain a complex mixture of volatile compounds that are responsible for the unique aromas of berries. However, there is very limited information on the composition and potential health benefits of berry volatiles. In this study, we isolated phenolic and volatile fractions from six common berries and characterized them by HPLC/HPLC-MS and GC/GC-MS, respectively. Berry phenolic and volatile fractions were evaluated for an anti-inflammatory effect using lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells by measuring levels of pro-inflammatory cytokines and the nuclear factor-kappa B (NF-κB) signaling pathway. Results showed that LPS-induced excessive production of nitric oxide (NO), prostaglandin E2 (PGE2), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), which were inhibited by berry phenolic and volatile extracts. Moreover, berry phenolic and volatile extracts reduced the nuclear translocation of NF-κB by blocking the phosphorylation of p65 and degradation of IκBα. These findings showed that berry volatiles from six berries had comparable anti-inflammatory effects to berry phenolics through the suppression of pro-inflammatory mediators and cytokines expression via NF-κB down-regulation, despite being present in the fruit at a lower concentration.

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“…Little research has been conducted on the anti-inflammatory effect of blackberry volatiles, but studies have demonstrated the anti-inflammatory capacities of essential oils that contain common blackberry volatiles (Hirota et al, 2010;Huo et al, 2013;Plastina et al, 2018). Moreover, in a recent study by Moore et al (2019), phenolic and volatile extracts isolated from cranberries, as well as each of the four most abundant volatiles in cranberries, αterpeniol, linalool oxide, eucalyptol, and linalool were tested at concentrations found in fresh cranberries for their anti-inflammatory capacities, before or after inflammation of RAW264.7 murine macrophage cells. When applied as a treatment after induction of inflammation in the cells, not only did the cranberry volatiles decrease nitric oxide (NO) production by the inflamed cells, but their effect was comparable to that of cranberry phenolics, even though they were 353 times less concentrated.…”

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confidence: 99%

Blackberry phenolic and volatile extracts inhibit cytokine secretion in LPS-inflamed RAW264.7 cells

Morin¹,

Howard²,

Tipton³

et al. 2021

JFB

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The anti-inflammatory activity of blackberries has been attributed to phenolic compounds, especially anthocyanins. The present study hypothesized that volatiles could contribute to anti-inflammatory activity as well. The anti-inflammatory properties of three blackberry genotypes varying in total volatile and phenolic contents were assessed by measuring concentrations of nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor -α (TNF-α) within LPS-inflamed RAW264.7 murine macrophage cells after a preventive treatment of either a phenolic or a volatile extract. Extracts from blackberry genotypes A2528T, A2587T and Natchez had total phenolic contents of 4315, 3369 and 3680 µg/mL, respectively, and total volatile contents of 283, 852 and 444 ng/mL, respectively. Phenolic and volatile extracts of all genotypes significantly lowered the secretion of NO, IL-6 and TNF-α in ranges varying between 20-42%, 34-60% and 28-73% inhibition, respectively. Volatile extracts exhibited greater anti-inflammatory properties than phenolic extracts, despite being present at much lower concentrations in the berries. Further research is needed to assess bioavailability and anti-inflammatory effect of blackberry volatiles in vivo.

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Inhibitory effects of cranberry polyphenol and volatile extracts on nitric oxide production in LPS activated RAW 264.7 macrophages

Abstract: Cranberry volatiles have received little attention for health-promoting properties.

Cited by 27 publications

References 37 publications

Anti-Inflammatory and Antioxidant Effects of Soroseris hirsuta Extract by Regulating iNOS/NF-κB and NRF2/HO-1 Pathways in Murine Macrophage RAW 264.7 Cells

Anti-Inflammatory and Antioxidant Effects of Soroseris hirsuta Extract by Regulating iNOS/NF-κB and NRF2/HO-1 Pathways in Murine Macrophage RAW 264.7 Cells

Berry Phenolic and Volatile Extracts Inhibit Pro-Inflammatory Cytokine Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κB Signaling Pathway

Blackberry phenolic and volatile extracts inhibit cytokine secretion in LPS-inflamed RAW264.7 cells

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