Inhibitory effects of constituents of Morinda citrifolia seeds on elastase and tyrosinase

Abstract

A 50% ethanolic extract (MCS-ext) from seeds of Morinda citrifolia ("noni" seeds) showed more potent in vitro inhibition of elastase and tyrosinase, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than extracts of M. citrifolia leaves or flesh. Activity-guided fractionation of MCS-ext using in vitro assays led to the isolation of ursolic acid as an active constituent of elastase inhibitory activity. 3,3'-Bisdemethylpinoresinol, americanin A, and quercetin were isolated as active constituents having both tyrosinase inhibitory and radical scavenging activities. Americanin A and quercetin also showed superoxide dismutase (SOD)-like activity. These active compounds were isolated from noni seeds for the first time.

Matrix Metalloproteinase-1 Inhibitory Activities of Morinda citrifolia Seed Extract and Its Constituents in UVA-Irradiated Human Dermal Fibroblasts

“…Effects of Constituents of MCS-ext and EGCG on MMP-1 Secretion from UVA-Irradiated NHDFs As reported in our previous paper, 10) 3,3′-bisdemethylpinoresinol (1, isolation yield from MCS-ext: 1.17%; isolation yield from dried seeds: 0.065%), americanin A (2, 0.51%; 0.024%), quercetin (3, 0.19%; 0.009%) and ursolic acid (4, 0.74%; 0.035%) have been isolated from the ethyl acetate-soluble fraction. The effects of these constituents on MMP-1 secretion were examined by Western blot analysis, and the relative intensity of each blot shown in Fig.…”

“…Compounds 2 and 3 also showed superoxide dismutase like activity. 10) If MCS-ext is proven to be MMP-1 inhibitor, an extract with HLE inhibitory activity may be an ideal ingredient for anti-photoaging cosmetics. The objective of this study was to determine if MCS-ext and its constituents have MMP-1 inhibitory activity in UVA-irradiated normal human dermal fibroblasts (NHDFs).…”

Aqueous Morinda citrifolia Leaves Extract Enhancing Glutathione Peroxidase Activity and α2-Macroglobulin Gene Expression on Macrobrachium rosenbergii

“…This small evergreen tree or shrub is native from Southeastern Asia (Indonesia) to Australia and now has a pantropical distribution. Various parts of noni plant extracts have been reported to have many significant effect such as fruit extract of M. citrifolia as antibacterial, antifungal, tumor suppression (Jayaraman et al, 2008), antioxidative activities from root of M. citrifolia (Zin et al, 2000), inhibitor on elastase and tyrosinase from seeds of M. citrifolia (Masuda et al, 2009) and also originally, the leaves were applied directly to the skin to treat ulcerations and minor infections (Usha et al, 2010). Nworu et al (2012) explained that Noni is a very popular for immune boosting reported to be beneficial in immunosuppression tumor, and in other immuno-inflammatory disorders.…”

Effect of Potato Starch on the Antioxidant Properties of Morinda Citrifolia Dried Micro Particles

“…It is used in traditional and/or modern application, including root and bark (dyes, medicine), trunks (firewood, tools), and leaves and fruits (food, medicine) although most of these have yet to be scientifically supported. Fruit, leave and root are useful in cardiovascular diseases particularly hypertension, atherosclerosis and dyslipidemia [10] while seed may have a role as a useful ingredient in cosmetics for skin-whitening and/ or wrinkle prevention [11]. In order to meet the demand of the market throughout the year in all areas, MorindaCitrifolia is perishable and cannot be marketed as fresh products.…”