Inhibitory effects of Cissus barbeyana leaf extracts on α-amylase and α-glucosidase in vitro

Obidike, Ifeoma; Salawu, OA

doi:10.1055/s-0030-1265855

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“…The genus Cissus (Vitaceae), also known as Vitis, consists of 350 species, widely distributed in China, India, Bangladesh, Nepal, and Southeast Asia [5]. Several species of Cissus, including C. cornifolia, C. barbeyana, C. repens, C. vinifera and C. verticillata, have been reported for antidiabetic potential [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Bergenin from <i>Cissus javana</i> DC. (Vitaceae) root extract enhances glucose uptake by rat L6 myotubes

San¹,

Boonsnongchee²,

Putalun³

et al. 2020

Trop. J. Pharm Res

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Purpose: To examine the glucose uptake stimulatory activity of the root extract of Cissus javana DC. (Vitaceae) in Lδ myotubes of rat, and also to identify the extract’s active principles.Methods: The methanol extract was prepared from Cissus javana tuberous roots and evaluated for glucose uptake stimulatory effects on Lδ rat muscle cells and inhibitory activity against α-glucosidase. The chemical components were isolated using several chromatographic techniques, and their structures characterized by spectroscopic methods. Each isolate was then assayed for glucose uptake stimulatory and α-glucosidase inhibitory activities.Results: The extract (100 μg/ml) exhibited glucose uptake stimulatory effect (70.9 % enhancement) and α-glucosidase enzyme inhibitory activity (100 % inhibition). Through chromatographic separation, bergenin, stigmast-4-en-3-one and β-sitosterol were isolated and identified. Bergenin, at 100 μg/ml (0.3046 mM), increased glucose uptake by Lδ myotubes by 50.5 % without toxicity. At the same concentration, bergenin showed no inhibition on α-glucosidase enzyme, while stigmast-4-en-3-one and β-sitosterol exhibited 98.6 and 40.6 %, inhibition, respectively.Conclusion: This study is the first report on the chemical constituents, and the glucose uptake stimulatory and α-glucosidase inhibitory activities of Cissus javana DC. roots. The findings reveal the antidiabetic potential of the plant and the glucose-uptake enhancing activity of bergenin. Keywords: Cissus javana, α-Glucosidase, Antidiabetes, Rat skeletal muscle cells, Bergenin

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Section: Introductionmentioning

confidence: 99%