Inhibitory Effects of Carbohydrates on Histamine Release and Mast Cell Disruption by Dextran

Beraldo, W. T.; Silva, W. Dias Da; Fernandes, A. D. Lemos

doi:10.1111/j.1476-5381.1962.tb01445.x

Cited by 31 publications

(20 citation statements)

References 19 publications

(7 reference statements)

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“…In contrast, addition of PS was not required for dextran to release histamine in vitro from other rat tissues, e.g. skin (Beraldo, Dias da Silva & Lemos Fernandes, 1962) or mesentery (Goth, 1961). Moreover PS does not appreciably augment antigen-induced release from sensitized rat mesentery or lung (Mongar & Svec, 1972 Goth & Knoohuizen (1962) to the low incubation temperature (20-240 C) which they used.…”

Section: Discussionmentioning

confidence: 99%

Inhibition by Cromoglycate of Histamine Release From Rat Peritoneal Mast Cells Induced by Mixtures of Dextran, Phosphatidyl Serine and Calcium Ions

Garland¹,

Mongar²

1974

British J Pharmacology

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1Dextran releases histamine from rat peritoneal mast cells in the presence but not in the absence of phosphatidyl serine (PS). With PS (10 ,g/ml) present the effect of dextran was concentration-dependent in the range of 0.2-6 mg/ml. 2 PS releases little histamine on its own but mixed with dextran (6 mg/ml) produces a graded effect in the range of 0.3-10 Mg/ml.3 The combination of dextran and PS releases no histamine in a medium containing less than 0.1 mm calcium. As the calcium concentration is increased, release occurs and a maximum is reached at calcium 1 mM; at higher concentrations release falls off sharply.4 Histamine release by the combination of dextran (6 mg/ml), PS (10 mg/ml) and calcium (1.8 mM) is inhibited by cromoglycate in the same range (1-30 gM) that inhibits anaphylactic histamine release from rat peritoneal cells. 5 Inhibition by cromoglycate occurred with all dextran concentrations tested (0.2-15 mg/ml) but high concentrations of PS (30 and 100 Mg/ml) overcame the effect of cromoglycate (2 and 10 uM). Calcium concentrations above 1 mM augmented inhibition by cromoglycate 2 and 10 AM.

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Section: Discussionmentioning

confidence: 99%

Inhibition by Cromoglycate of Histamine Release From Rat Peritoneal Mast Cells Induced by Mixtures of Dextran, Phosphatidyl Serine and Calcium Ions

Garland¹,

Mongar²

1974

British J Pharmacology

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“…1971), desensitization and cross-desensitization with antigen (Foreman & Garland, 1974;Foreman, Garland & Mongar, 1976) and inhibition by antiallergic agents such as cromoglycate (Garland & Mongar, 1974). Furthermore, the reaction is diminished by metabolic inhibitors such as 2,4-dinitrophenol (Beraldo, Dias da Silva & Lemos Fernandes, 1962).…”

Section: Introductionmentioning

confidence: 99%

The Relationship Between Energy Metabolism and the Action of Inhibitors of Histamine Release

Garland

Johansen

1977

British J Pharmacology

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1Dextran-induced release of histamine from rat mast cells was inhibited equally in complete and glucose-free Tyrode solution by doxantrazole (0.03-3,mol/l), theophylline (0.1-3 mmol/l) and dicumarol (0.0 1-10 gmol/litre).2 Doxantrazole (3 gmol/1), theophylline (3 mmol/1) and dicumarol (10 pmol/1) did not reduce the adenosine 5'-triphosphate (ATP) content of mast cells in glucose-free medium. Higher concentrations of dicumarol (56-100 gmol/1) markedly reduced the cellular ATP content. This reduction was reversed by glucose. 3 Papaverine was a more potent inhibitor of histamine release from mast cells incubated in glucosefree solution than in complete Tyrode solution (dose-ratio = 20). Like antimycin A (1 ,umol/l), papaverine (3 Amol/l) caused a depletion of mast cell ATP that was greater in the absence (85%) than in the presence (25%) ofextracellular glucose. 4 These results suggest that dicumarol, like doxantrazole and theophylline, inhibits histamine release without affecting mast cell energy metabolism. In contrast, papaverine probably inhibits release by depleting ATP that is required for exocytosis. SInhibition of histamine release by dibutyryl cyclic adenosine 3,5'-monophosphate (1-3 mmol/l) was significantly greater when cells were incubated in complete rather than in glucose-free medium.

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confidence: 99%

“…These sugars also prevent the local vascular changes produced by other polysaccharides (Poyser & West, 1965;Harris, Luscombe & Poyser, 1967) and inhibit the release of histamine in vitro by dextran from tissue mast cells (Goth, 1961;Beraldo et al, 1962;Dias Da Silva & Lemos Fernandes, 1965). In the present paper, an attempt has been made to determine the structural and stereospecific requirements of sugars for activity as antagonists of the dextran response.…”

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Structural Requirements of Sugars as Antagonists of the Vascular Response to Dextran in Rat Skin

Poyser

West

1968

British Journal of Pharmacology and Chemotherapy

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The increase in vascular permeability induced by dextran in rat skin is inhibited by the simultaneous intradermal administration of glucose and certain other sugars (Beraldo, Dias Da Silva & Lemos Fernandes, 1962). These sugars also prevent the local vascular changes produced by other polysaccharides (Poyser & West, 1965;Harris, Luscombe & Poyser, 1967) and inhibit the release of histamine in vitro by dextran from tissue mast cells (Goth, 1961;Beraldo et al., 1962;Dias Da Silva & Lemos Fernandes, 1965). In the present paper, an attempt has been made to determine the structural and stereospecific requirements of sugars for activity as antagonists of the dextran response. The mechanism of inhibition by these carbohydrates has also been investigated. METHODSInhibition by sugars and other agents of the vascular response to dextran in rat skin was studied using the method of Poyser & West (1965). Each antagonist was dissolved in dextran (1 mg/ml.) and injected intradermally, in volumes of 0.1 ml., into the shaved ventral abdominal skin of male hooded Lister rats (body weight about 200 g). Each rat received eight to ten intradermal injections immediately after the intravenous injection of azovan blue dye (7 mg/kg). Thirty minutes later the rats were killed, and the reaction to each injection was assessed by measuring the mean diameter of the blue area on the inner surface of the skin. The response given by dextran (100 jug) in the presence of each sugar or other agent was then expressed as a percentage of that found in its absence. From the log dose/inhibition curves, doses producing 50% inhibition (termed the ID50 values) were obtained. Each value shown in the tables is the mean and standard error of three experiments, each using at least three rats.

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Inhibitory Effects of Carbohydrates on Histamine Release and Mast Cell Disruption by Dextran

Cited by 31 publications

References 19 publications

Inhibition by Cromoglycate of Histamine Release From Rat Peritoneal Mast Cells Induced by Mixtures of Dextran, Phosphatidyl Serine and Calcium Ions

Inhibition by Cromoglycate of Histamine Release From Rat Peritoneal Mast Cells Induced by Mixtures of Dextran, Phosphatidyl Serine and Calcium Ions

The Relationship Between Energy Metabolism and the Action of Inhibitors of Histamine Release

Structural Requirements of Sugars as Antagonists of the Vascular Response to Dextran in Rat Skin

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