Inhibitory Effects of Carbazomycin B Produced by Streptomyces roseoverticillatus 63 Against Xanthomonas oryzae pv. oryzae

Shi, Tingting; Guo, Xin; Zhu, Jiali; Hu, Lingming; He, Zhisong; Jiang, Donghua

doi:10.3389/fmicb.2021.616937

Cited by 10 publications

(8 citation statements)

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“…S. nematodiphila GCSR38 secondary metabolite extracts can reduce the disease severity of BLB in vitro and in vivo. This is similar to the findings of Shi et al, 43 who reported the role of Streptomyces roseoverticillatus 63 (Sr-63) against Xoo; disease suppression was accomplished under glasshouse conditions. In addition, carbazomycin B isolated from the fermented broth of strain Sr-63 was found to be active against Xoo, with an MIC of 8 µg/ml.…”

Section: Discussionsupporting

confidence: 90%

Comparison of the Antagonistic Potential of the Entomopathogenic Bacterium Serratia nematodiphila GCSR38 with other Effective Microorganisms for the Control of Rice Bacterial Leaf Blight

Sutthisa¹

2022

J. Pure Appl. Microbiol.

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The efficacy of antagonistic microorganisms and secondary metabolites of entomopathogenic bacteria was evaluated in vitro and in vivo against Xanthomonas oryzae pv. oryzae (Xoo), a causal agent of rice bacterial leaf blight. The effect of the culture filtrates of the microorganisms was tested against Xoo, using the agar well diffusion assay. Trichoderma sp. UB05/3 was the most effective against Xoo. However, the results were not statistically different from those of the entomopathogenic bacterium Serratia nematodiphila GCSR38 with a 22.97 mm and 19.15 mm zone of inhibition, respectively. The effect of the secondary metabolite crude extracts of antagonistic microorganisms (2,000 µg/ml) on Xoo inhibition was tested by the paper disc diffusion method. Only S. nematodiphila GCSR38 was able to control Xoo, with an inhibition zone of 17.60 mm. Minimum inhibitory concentration (MIC) was determined using a 96-well microtiter plate. The MIC of secondary metabolites crude extracts of S. nematodiphila GCSR38 was 1,000 µg/ml and the minimum bactericidal concentration (MBC) was 1,000 µg/ml. The efficacy of the secondary metabolite crude extract of S. nematodiphila GCSR38 against Xoo was tested using the detached leaf technique; the secondary metabolite crude extracts controlled the disease, with 24.45% and 15.56% disease severity when used before and after inoculation with Xoo, respectively. Inoculation with Xoo alone resulted in a disease severity of 44.45%. The secondary metabolite crude extracts of S. nematodiphila GCSR38 can reduce disease severity of bacterial leaf blight in rice by 28.89%, whereas zinc thiazole causes a disease severity of 22.22% and Xoo alone causes a disease severity of 66.67%.

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Section: Discussionsupporting

confidence: 90%

Comparison of the Antagonistic Potential of the Entomopathogenic Bacterium Serratia nematodiphila GCSR38 with other Effective Microorganisms for the Control of Rice Bacterial Leaf Blight

Sutthisa¹

2022

J. Pure Appl. Microbiol.

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“…It was grown on Gauze’s synthetic broth medium no. 1 ( Shi et al, 2021 ) for activation and then inoculated into DBY medium (glucose 20 g·L −1 , soybean flour 5 g·L −1 , yeast flour 4 g·L −1 , ammonium sulfate 5 g·L −1 , NaCl 1 g·L −1 , and K 2 HPO 4 0.05 g·L −1 ) for lipopeptide production. Magnaporthe oryzae Guy11 was routinely cultured on PDA (B; Virna et al, 2010 ) medium, and oatmeal-tomato juice agar medium (OMTA; Nie et al, 2014 ) was used for spore production.…”

Section: Methodsmentioning

confidence: 99%

Genomic and Biocontrol Potential of the Crude Lipopeptide by Streptomyces bikiniensis HD-087 Against Magnaporthe oryzae

Liu

Wang

et al. 2022

Front. Microbiol.

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Rice blast caused by Magnaporthe oryzae is one of the most destructive plant diseases. The secondary metabolites of Streptomyces have potential as biological control agents against M. oryzae. However, no commercial secondary antimicrobial products of Streptomyces have been found by gene prediction, and, particularly relevant for this study, a biocontrol agent obtained from Streptomyces bikiniensis has yet to be found. In this research, genomic analysis was used to predict the secondary metabolites of Streptomyces, and the ability to develop biocontrol pharmaceuticals rapidly was demonstrated. The complete genome of the S. bikiniensis HD-087 strain was sequenced and revealed a number of key functional gene clusters that contribute to the biosynthesis of active secondary metabolites. The crude extract of lipopeptides (CEL) predicted by NRPS gene clusters was extracted from the fermentation liquid of S. bikiniensis HD-087 by acid precipitation followed by methanol extraction, and surfactins, iturins, and fengycins were identified by liquid chromatography-mass spectrometry (LC–MS). In vitro, the CEL of this strain inhibited spore germination and appressorial formation of M. oryzae by destroying membrane integrity and through the leakage of cellular components. In vivo, this CEL reduced the disease index of rice blast by approximately 76.9% on detached leaves, whereas its control effect on leaf blast during pot experiments was approximately 60%. Thus, the S. bikiniensis CEL appears to be a highly suitable alternative to synthetic chemical fungicides for controlling M. oryzae.

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“…Gauze’s synthetic broth medium No. 1 [ 20 ] was used for strain HD-087 activation, DBY medium [ 19 ] was used for HD-087 fermentation for lipopeptide production, and PDB medium [ 21 ] was used for Guy11 culture.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome analysis of the production enhancement mechanism of antimicrobial lipopeptides of Streptomyces bikiniensis HD-087 by co-culture with Magnaporthe oryzae Guy11

et al. 2022

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The lipopeptides produced by Streptomyces bikiniensis have a significant inhibitory effect on Magnaporthe oryzae, but the low yield limits its application. In this study, the anti-M. oryzae activity of the broth of S. bikiniensis HD-087 co-cultured with M. oryzae Guy11 mycelium has risen by 41.22% compared with pure culture, and under induction conditions of adding Guy11-inducer (cell-free supernatant of M. oryzae Guy11), the activity of strain HD-087 improved 61.76%. The result proved that the enhancement effect of Guy11 on the antimicrobial activity of HD-087 was mainly related to metabolites but mycelium cells. Under optimum induction conditions, NRPS gene expression levels of HD-087 were significantly increased by induction with Guy11-inducer, the biomass of HD-087 had no significant change, but crude extract of lipopeptide (CEL) production was 107.4% higher than pure culture, and TLC result under acid hydrolysis showed that the induced culture has one component more than pure culture. To clarify the regulation mechanism of improving lipopeptide production of HD-087 with Guy11-inducer, transcriptomic analysis was performed using RNAseq to compare the induced culture and pure culture. In the induced culture, 943 genes were up-regulated, while 590 genes were down-regulated in DEGs (differentially expressed genes). KEGG results showed that the expression of genes related to amino acid synthesis, fatty acid metabolism, TCA cycle and pyruvate metabolism pathway were significantly increased. The increased expression of genes related to these metabolic pathways provided sufficient precursors for lipopeptide synthesis. Accordingly, key enzyme genes responsible for the synthesis of lipopeptides Srf and NRPS was significantly increased. Quorum sensing related genes OppA and MppA were significantly up-regulated, and then ComP was activated and promoted lipopeptide synthesis. These results provided a scientific basis for using M. oryzae to induce the increase of the production of Streptomyces lipopeptides, and also laid a foundation for further exploring the co-culture mechanisms among different genera.

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Inhibitory Effects of Carbazomycin B Produced by Streptomyces roseoverticillatus 63 Against Xanthomonas oryzae pv. oryzae

Cited by 10 publications

References 50 publications

Comparison of the Antagonistic Potential of the Entomopathogenic Bacterium Serratia nematodiphila GCSR38 with other Effective Microorganisms for the Control of Rice Bacterial Leaf Blight

Comparison of the Antagonistic Potential of the Entomopathogenic Bacterium Serratia nematodiphila GCSR38 with other Effective Microorganisms for the Control of Rice Bacterial Leaf Blight

Genomic and Biocontrol Potential of the Crude Lipopeptide by Streptomyces bikiniensis HD-087 Against Magnaporthe oryzae

Transcriptome analysis of the production enhancement mechanism of antimicrobial lipopeptides of Streptomyces bikiniensis HD-087 by co-culture with Magnaporthe oryzae Guy11

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