Inhibitory effects of a super pulsed carbon dioxide laser at low energy density on periodontopathic bacteria and lipopolysaccharide <i>in vitro</i>

Kojima, Takanori; Shimada, Koji; Iwasaki, Hiroyasu; Ito, Koichi

doi:10.1111/j.1600-0765.2005.00826.x

Cited by 28 publications

(31 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it is difficult to remove plaque completely using conventional methods. Novel methods, such as the use of some chemical agents, laser irradiation, and both sonic and ultrasonic treatments, may be useful for controlling plaque (1,2,3,20,45,46). Therapeutic ultrasound, which has a long history of use as a therapeutic, diagnostic, and surgical tool (4,5,7,38), uses sound waves to transfer mechanical energy to tissues and cells.…”

mentioning

confidence: 99%

Inhibitory Effects of Low-Energy Pulsed Ultrasonic Stimulation on Cell Surface Protein Antigen C through Heat Shock Proteins GroEL and DnaK inStreptococcus mutans

Ishibashi

Shimada²,

Kawato

et al. 2010

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

This study concerns the use of low-energy pulsed ultrasound as nondestructive photodynamic antimicrobial therapy for controlling dental plaque. We examined the antibacterial and bactericidal effects of low-energy pulsed ultrasound on mutans streptococci and its inhibitory effects on bacterial cell adhesion of Streptococcus mutans. The results indicated weak antibacterial and bactericidal effects. However, ultrasonic stimulation for less than 20 min markedly decreased bacterial cell adhesion. To analyze the mechanism underlying the inhibitory effect, we examined cell surface protein antigen C (PAc) and glucosyltransferase I (GTF-I) expression in S. mutans. The levels of PAc gene and protein expression were markedly decreased by ultrasonic stimulation for 20 min. However, no change in GTF-I expression was observed. The expression of stress response heat shock proteins GroEL and DnaK was also examined. GroEL and DnaK levels were significantly decreased by ultrasonic stimulation, and the expression of the PAc protein was also diminished upon the addition of GroEL or DnaK inhibitors without ultrasonic stimulation. These observations suggest that the expression of the PAc protein in S. mutans may be dependent on heat shock proteins. Thus, low-energy pulsed ultrasound decreases bacterial adhesion by the inhibitory effect on the PAc protein and heat shock protein expression and may be useful as photodynamic antimicrobial chemotherapy in controlling dental plaque.

show abstract

mentioning

confidence: 99%

Inhibitory Effects of Low-Energy Pulsed Ultrasonic Stimulation on Cell Surface Protein Antigen C through Heat Shock Proteins GroEL and DnaK inStreptococcus mutans

Ishibashi

Shimada²,

Kawato

et al. 2010

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The bacteria are inactivated, 24,25 and dehydration occurs as energy is applied. Delivery by an articulated arm in noncontact mode facilitates treatment.…”

Section: Micropulsed Co 2 Lasermentioning

confidence: 99%

“…Fluids increase within the tissues, circulation becomes stagnant, swelling occurs, and metabolic products become backlogged. 24,25 Both CO 2 and erbium lasers act on pathogens by heating intracellular fluids, causing the microbes to collapse. The inflamed tissue is unable to progress from the granulation phase of healing into the remodeling phase 27 Laser-Assisted Nonsurgical Periodontal Therapy red and orange-complex bacteria associated with periodontitis.…”

mentioning

confidence: 99%

Laser-Assisted Nonsurgical Periodontal Therapy

Smith¹

2011

Principles and Practice of Laser Dentistry

View full text Add to dashboard Cite

“…Kojima et al . reported that the in vitro use of a CO 2 laser killed more than 99% of Porphyromonas gingivalis (PG) and Aggregatibacter actinomycetemcomitans (AA) at 7.5 and 12.5 J/cm 2 and significantly reduced LPS biological activity when irradiated by energy densities greater than 7.5 J/cm 2 [9]. However, there is insufficient evidence to conclude if a laser can decontaminate a periodontal pocket [10].…”

Section: Introductionmentioning

confidence: 99%

Laser Assisted Non-surgical Periodontal Therapy: A Double Blind, Randomized Clinical Trial

Everett¹,

Rossmann²,

Kerns³

et al. 2017

TODENTJ

View full text Add to dashboard Cite

The objective of this study was to examine potential benefits of using laser therapy for secular decontamination in conjunction with scaling and root planing in the treatment of chronic periodontitis. The study was performed on 173 teeth in 14 patients in a split-mouth design, one side received scaling and root planing followed by laser therapy using a carbon dioxide (CO2) laser with an ablative handpiece (test group); the contralateral side received scaling and root planing without laser (control group). Clinical and laboratory parameters were evaluated prior to treatment and at 3 and 6 months following therapy; clinical measurements were performed by two blinded examiners. The clinical parameters included measurement of gingival recession (REC), bleeding on probing (BOP), clinical attachment level (CAL), pocket depth (PD), furcation involvement (FUR), and tooth mobility (MOB). Laboratory testing to determine the levels of periodontal pathogens was performed using PCR techniques. The results of the study revealed statistically significant differences in clinical and laboratory parameters at 3 and 6 months after therapy for both test and control groups, but no significant difference was observed between the two groups. However, sites receiving laser therapy tended to show a greater decrease in probing depths, gain in clinical attachment level, and reduced bacterial levels. In conclusion, the overall results of the study suggest a potential benefit of using laser therapy in conjunction with scaling and root planing for the treatment of chronic periodontitis.

show abstract

Inhibitory effects of a super pulsed carbon dioxide laser at low energy density on periodontopathic bacteria and lipopolysaccharide in vitro

Abstract: The results indicate that CO2 laser irradiation at low power is capable of bactericidal effect on periodontopathic bacteria and decreasing LPS activity.

Cited by 28 publications

References 31 publications

Inhibitory Effects of Low-Energy Pulsed Ultrasonic Stimulation on Cell Surface Protein Antigen C through Heat Shock Proteins GroEL and DnaK inStreptococcus mutans

Inhibitory Effects of Low-Energy Pulsed Ultrasonic Stimulation on Cell Surface Protein Antigen C through Heat Shock Proteins GroEL and DnaK inStreptococcus mutans

Laser-Assisted Nonsurgical Periodontal Therapy

Laser Assisted Non-surgical Periodontal Therapy: A Double Blind, Randomized Clinical Trial

Contact Info

Product

Resources

About