Inhibitory effects of a Kunitz-type inhibitor from Pithecellobium dumosum (Benth) seeds against insect-pests' digestive proteinases

Exploiting the biological roles of the trypsin inhibitor from Inga vera seeds: A multifunctional Kunitz inhibitor

“…Among them, Kunitz trypsin inhibitor from Piptadenia moniliformis (PmTKI) inhibited trypsin-like enzymes of lepidopteran larvae, such as Anthonomus grandis (90%), Plodia interpunctella (60%) and of the fly Ceratitis capitata (70.5%, Diptera) [16]. In the case of PdKI-4 (Pithecellobium dumosum Kunitz-type trypsin inhibitor), a higher activity was observed against the digestive enzymes from Callosobruchus maculatus (66%) and Zabrotes subfasciatus (60%), which belong to order Coleoptera [58]. These data reinforce the fact that the inhibitory effects of PIs on insect enzymes vary and demonstrate the importance of preliminary screening to select better targets for pest control.…”

Purification and Partial Characterization of Trypsin-Specific Proteinase Inhibitors from Pigeonpea Wild Relative Cajanus platycarpus L. (Fabaceae) Active against Gut Proteases of Lepidopteran Pest Helicoverpa armigera

“…The studies of Hogg (2003) reported that disulphide bonds have been added to the PIs during evolution to enhance the protein stability, which in turn prevent their digestion by cellular proteases and thereby increase their half-life by molecular packing. Stability against high temperature and pH was also observed with other group of PIs from seeds of Dolichus biflorus (Kumar and Gowda, 2013), Pithecellobium dumosum (Rufino et al, 2013), and Putranjiva roxburghii (Chaudhary et al, 2008). …”

“…These results imply that isoinhibitors of CpPI 63 present at ~19 kDa region might exist in a combination of both as a single as well as two polypeptides chain isoinhibitors (Ee et al, 2009; Rufino et al, 2013). The identification of high molecular mass TIs in SDS-PAGE, MALDI intact mass analysis, and 2-D activity staining studies together suggest the possibility of existence of MLPs in CpPI 63.…”

Enhancement of tomato resistance to Tuta absoluta by the expression of two barley proteinase inhibitors

“…Kunitz type inhibitors have been described in legumes, cereals and solanaceaous species (Laskowski Jr & Kato, 1980;Ishikawa et al, 1994;Cruz et al, 2013;Rufino et al, 2013). These enzymes are produced under stress.…”

“…Subsequently, other PIs were expressed in other plants conferring them resistance to a wide range of pests (Carrillo et al, 2011;Saadati & Bandani, 2011;Rufino et al, 2013;Quilis et al, 2014). The most studied are SPIs, however it has been suggested that cysteine PIs and aspartate PIs act in the same way according to the mechanism proposed by Laskowski and Kato (1980 (Estell et al, 1980;Haq et al, 2004) …”