Inhibitory effect of ZnCl2 on glycolysis in human oral microbes

He, Guobin; Pearce, E.I.F.; Sissons, C.H.

doi:10.1016/s0003-9969(01)00093-0

Cited by 66 publications

(53 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As shown in Fig.1b, when the lysozyme independently infl uenced the biofi lm, the inhibition ratio of biofi lm was very low. This phenomenon may be because a zinc ion can activate a particular part of lysozyme which can more eff ectively increase the inhibition ratio of lysozyme (He et al, 2002;Burguera-Pascu et al, 2007), but no signifi cant inhibition of vitality was observed at high concentrations, which is in accordance with the report by Gu et al (2012). Because of this reason, ZnSO 4 continued to be used in the following experiment.…”

Section: Discussionsupporting

confidence: 73%

Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash

Ren

Wang

et al. 2015

Chin. J. Ocean. Limnol.

View full text Add to dashboard Cite

We adopted the response surface methodology using single factor and orthogonal experiments to optimize four types of antimicrobial agents that could inhibit biofi lm formation by Streptococcus mutans , which is commonly found in the human oral cavity and causes tooth decay. The objective was to improve the function of marine Arthrobacter oxydans KQ11 dextranase mouthwash (designed and developed by our laboratory). The experiment was conducted in a three-level, four-variable central composite design to determine the best combination of ZnSO 4 , lysozyme, citric acid and chitosan. The optimized antibacterial agents were 2.16 g/L ZnSO 4 , 14 g/L lysozyme, 4.5 g/L citric acid and 5 g/L chitosan. The biofi lm formation inhibition reached 84.49%. In addition, microscopic observation of the biofi lm was performed using scanning electron microscopy and confocal laser scanning microscopy. The optimized formula was tested in marine dextranase Arthrobacter oxydans KQ11 mouthwash and enhanced the inhibition of S . mutans . This work may be promoted for the design and development of future marine dextranase oral care products.

show abstract

Section: Discussionsupporting

confidence: 73%

Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash

Ren

Wang

et al. 2015

Chin. J. Ocean. Limnol.

View full text Add to dashboard Cite

show abstract

“…The phosphoenolpyruvate:sugar phosphotransferase system and the protontranslocating ATPase are particularly sensitive to zinc inhibition, and this inhibition reduces sugar transport and acid tolerance (2,7,22). Zinc is largely bacteriostatic, although very high concentrations can have a bactericidal effect (12,22). The addition of zinc to -casein-A(106-169) in a 1:1 ratio at neutral pH produced an antibacterial effect against S. mutans greater than that of zinc alone (Table 1, Fig.…”

Section: Discussionmentioning

confidence: 99%

Divalent Metal Cations Increase the Activity of the Antimicrobial Peptide Kappacin

Dashper

O'Brien-Simpson

Cross

et al. 2005

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Kappacin, nonglycosylated -casein(106-169), is a novel antimicrobial peptide produced from -casein found in bovine milk. There are two major genetic forms of kappacin, A and B, and using synthetic peptides corresponding to the active region, -casein(138-158), of these forms, we have shown that the Asp 148 to Ala 148 substitution is responsible for the lesser antibacterial activity of -casein-B(106-169). Kappacin was shown to have membranolytic action at concentrations above 30 M at acidic pH when tested against artificial liposomes. There was little membranolytic activity at neutral pH, which is consistent with the lack of antibacterial activity of kappacin against Streptococcus mutans at this pH. Kappacin specifically bound two zinc or calcium ions per mol, and this binding enhanced antibacterial activity at neutral pH. Nuclear magnetic resonance analysis indicated that a -casein-A(138-158) synthetic peptide undergoes a conformational change in the presence of the membrane solvent trifluoroethanol and excess divalent metal ions. This change in conformation is presumably responsible for the increase in antibacterial activity of kappacin detected in the presence of excess zinc or calcium ions at neutral pH. When tested against the oral bacterial pathogen S. mutans cultured as a biofilm in a constant-depth film fermentor, a preparation of 10 g/liter kappacin and 20 mM ZnCl 2 reduced bacterial viability by 3 log 10 and suppressed recovery of viability. In contrast 20 mM ZnCl 2 alone reduced bacterial viability by Ϸ1 log 10 followed by rapid recovery. In conclusion, kappacin has a membranolytic, antibacterial effect that is enhanced by the presence of divalent cations.

show abstract

“…He vµ cs. [6] ph¸t hiÖn thÊy Zn øc chÕ sù sinh axit kh«ng chØ cña Streptococcus mµ cßn cña nhiÒu loµi vi khuÈn xoang miÖng kh¸c. GÇn ®©y, Phan vµ cs.…”

Section: Tr−êng ®¹I Häc Khoa Häc Tù Nhiªn §Hqghnunclassified

“…Tr−íc khi ®i vµo cßn ®−êng glycolysis, ®−êng ®−îc ho¹t hãa thµnh d¹ng glucoza-6-phètph¸t nhê hÖ thèng enzim chuyÓn gèc phètph¸t (sugar-phosphotransferase system) viÕt t¾t lµ PTS vµ hexokinaza, trong ®ã hÖ thèng PTS ®ãng vai trß chÝnh [2]. Sù øc chÕ qu¸ tr×nh sinh axit cña S. mutans bëi Zn 2+ ®· ®−îc ph¸t hiÖn trong c¸c nghiªn cøu tr−íc ®©y [6,10], gîi ý mèi liªn quan cña enzim PTS víi qu¸ tr×nh nµy. KÕt qu ¶ thu ®−îc ë h×nh 2 cho thÊy Zn 2+ ë nång ®é kh¸ thÊp (0,02 mM) ®· øc chÕ 50% ho¹t ®é cña PTS.…”

Section: H×nh 1 ¶Nh H−ëng Cña Znunclassified

Zinc effects on enzymes of oral bacteria

Phương¹,

Nghia²

2014

V J Bio

View full text Add to dashboard Cite

Inhibitory effect of ZnCl2 on glycolysis in human oral microbes

Cited by 66 publications

References 36 publications

Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash

Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash

Divalent Metal Cations Increase the Activity of the Antimicrobial Peptide Kappacin

Zinc effects on enzymes of oral bacteria

Contact Info

Product

Resources

About